Cohort Administration and Biorepository Core

项目摘要/摘要-队列管理和生物库核心 大量数据表明，住在农场与哮喘和特应性疾病风险降低有关。 疾病，包括来自欧洲和北美的研究。个人因素似乎是 与这种“农场生活效应”相关的因素包括食用未经巴氏灭菌的农场牛奶和接触农场。 动物和马厩。与城市儿童相比，农场儿童的调节性T细胞数量更多，而且 他们的先天免疫系统似乎也是这些暴露的目标。农场家庭的房屋灰尘已经 更大的微生物多样性，与哮喘的发病率呈强烈的负相关。因此，这些研究 FAR将重点放在学龄期哮喘的后果上。然而，特应性皮炎和食物过敏很常见 在所谓的“特应性进行曲”中先于呼吸道过敏的发展。我们在旧秩序中的学习 门诺派社区表示，他们患特应性疾病和婴儿肠道微生物群的风险较低 成分与罗切斯特婴儿不同。 队列管理和生物存储库核心将形成不同但 本U19计划的补充项目1-3，“特应性早起的生物标志物”(BABE)。《核心》 将对特应性风险极低的OOM新生儿进行跟踪和扩展正在进行的纵向出生队列研究 皮炎、食物过敏、花粉热和哮喘以及来自特应性家庭的罗切斯特新生儿 出现这些特应性表现。我们将检验婴儿肠道微生物群与 代谢途径和先天刺激促进了OOM的耐受和屏障功能。具体目标 核心目标是1)抽样和临床随访已经招募的1个婴儿，直到6岁 对于其他过敏性疾病，目标2)招募、抽样和临床随访类似队列2的120名婴儿 用于特应性皮炎和食物过敏的发展，直到24个月龄，并目的3)准备，储存和分享 项目1-3中使用的样本(脐带血和婴儿外周血、粪便、皮肤拭子和胶带)以评估 农耕生活方式对儿童肠道微生物群、先天性和获得性免疫成分及皮肤屏障的影响 功能和微生物群组成。 这些研究旨在为儿童早期过敏的一级和二级预防提供新的策略 疾病。

PROJECT SUMMARY/ABSTRACT – COHORT ADMINISTRATION & BIOREPOSITORY CORE A large body of data suggests that living on farms is associated with a decreased risk of asthma and atopic diseases, including studies from Europe and the North America. The individual factors that appear to be associated with this “farm-life effect” include consumption of unpasteurized farm milk and exposure to farm animals and stables. Farm children have higher numbers of regulatory T cells, compared to urban children, and their innate immune system also appears to be the target for these exposures. House dust in farm homes has greater microbial diversity that is strongly negatively associated with the incidence of asthma. The studies thus far have focused on outcomes of asthma at school-age. However, atopic dermatitis and food allergy commonly precede development of respiratory allergies in a so-called “atopic march”. Our studies in the Old Order Mennonite Community have indicated that they are at low risk for atopic diseases and the infant gut microbiome composition is different from Rochester infants. The Cohort Admin and Biorepository Core will form the sample and data infrastructure for the distinct but complementary Projects 1-3 of this U19 Program, “Biomarkers of Atopy Beginning Early” (BABE). The Core will follow up and expand an ongoing longitudinal birth cohort study of OOM neonates at a very low risk for atopic dermatitis, food allergies, hayfever and asthma and Rochester neonates from atopic families with a high risk for developing these atopic manifestations. We will test the overall hypothesis that infant gut microbiome associated metabolic pathways and innate stimulation promote tolerance and barrier function in the OOM. The specific aims of the Core are to Aim 1) sample and clinically follow up already recruited Cohort 1 infants until 6 years of age for additional allergic diseases, Aim 2) recruit, sample and clinically follow up a similar cohort 2 of 120 infants for the development of atopic dermatitis and food allergy until 24mo of age, and Aim 3) prepare, store and share samples utilized in the Projects 1-3 (cord and infant peripheral blood, stool, skin swabs and tape strips) to assess farming lifestyle effect on children's gut microbiome, innate and adaptive immune composition, and skin barrier function and microbiome composition. These studies aim for novel strategies for primary and secondary prevention of early childhood allergic diseases.