Cohort Administration and Biorepository Core

队列管理和生物样本库核心

• 批准号: 10633366
• 负责人: Antti Seppo
• 金额: $ 23.99万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-07 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10633366
• 关键词: 6 year old; Age; Allergens; Allergic; Allergic Disease; Asthma; Atopic Dermatitis; Biological Markers; Birth; Child; Childhood; Clinic; Clinical; Cohort Studies; Communities; Consumption; County; Data; Development; Diagnosis; Disease; Epigenetic Process; Epithelial Cells; Equipment and supply inventories; Europe; Exposure to; Family; Farm; Feces; Fingers; First Degree Relative; Food Hypersensitivity; German population; Hay fever; Home; House Dust; Hypersensitivity; Immune; Immunologic Markers; Immunology; Impairment; Incidence; Individual; Infant; Infant Development; Inflammatory; Innate Immune System; Insurance; Life; Life Style; Livestock; Measures; Medical; Mennonite; Metabolic Pathway; Metadata; Milk; North America; Outcome; Patient Self-Report; Permeability; Physicians; Population; Pregnant Women; Prevention strategy; Primary Prevention; Process; Production; Regulatory T-Lymphocyte; Research Personnel; Risk; Sampling; School-Age Population; Secondary Prevention; Siblings; Signs and Symptoms; Skin; Surveys; Swab; TSLP gene; Testing; Visit; Woman; atopy; biobank; biomarker identification; clinical development; cohort; comparison group; computerized data processing; cytokine; data infrastructure; early childhood; early onset; follow-up; food allergen; gut microbiome; high risk; infant gut microbiome; metabolome; microbial; microbiome composition; neonate; novel strategies; peripheral blood; prenatal; programs; recruit; research clinical testing; respiratory; sample collection; shared database; skin barrier; symposium; unpasteurized; urban children

PROJECT SUMMARY/ABSTRACT – COHORT ADMINISTRATION & BIOREPOSITORY CORE A large body of data suggests that living on farms is associated with a decreased risk of asthma and atopic diseases, including studies from Europe and the North America. The individual factors that appear to be associated with this “farm-life effect” include consumption of unpasteurized farm milk and exposure to farm animals and stables. Farm children have higher numbers of regulatory T cells, compared to urban children, and their innate immune system also appears to be the target for these exposures. House dust in farm homes has greater microbial diversity that is strongly negatively associated with the incidence of asthma. The studies thus far have focused on outcomes of asthma at school-age. However, atopic dermatitis and food allergy commonly precede development of respiratory allergies in a so-called “atopic march”. Our studies in the Old Order Mennonite Community have indicated that they are at low risk for atopic diseases and the infant gut microbiome composition is different from Rochester infants. The Cohort Admin and Biorepository Core will form the sample and data infrastructure for the distinct but complementary Projects 1-3 of this U19 Program, “Biomarkers of Atopy Beginning Early” (BABE). The Core will follow up and expand an ongoing longitudinal birth cohort study of OOM neonates at a very low risk for atopic dermatitis, food allergies, hayfever and asthma and Rochester neonates from atopic families with a high risk for developing these atopic manifestations. We will test the overall hypothesis that infant gut microbiome associated metabolic pathways and innate stimulation promote tolerance and barrier function in the OOM. The specific aims of the Core are to Aim 1) sample and clinically follow up already recruited Cohort 1 infants until 6 years of age for additional allergic diseases, Aim 2) recruit, sample and clinically follow up a similar cohort 2 of 120 infants for the development of atopic dermatitis and food allergy until 24mo of age, and Aim 3) prepare, store and share samples utilized in the Projects 1-3 (cord and infant peripheral blood, stool, skin swabs and tape strips) to assess farming lifestyle effect on children's gut microbiome, innate and adaptive immune composition, and skin barrier function and microbiome composition. These studies aim for novel strategies for primary and secondary prevention of early childhood allergic diseases.

项目概要/摘要-队列管理和生物库核心 大量数据表明，生活在农场与哮喘和特应性哮喘的风险降低有关。 包括欧洲和北美的研究。这些个体因素似乎 与这种“农场生活效应”有关的包括食用未经巴氏消毒的农场牛奶和接触农场 动物和马厩与城市儿童相比，农村儿童的调节性T细胞数量更多， 他们的先天免疫系统似乎也是这些接触的目标。农场房屋的灰尘 更大的微生物多样性与哮喘的发病率呈强烈的负相关。因此，研究 目前主要关注学龄期哮喘的结果。然而，特应性皮炎和食物过敏通常 在所谓的“特应性进行曲”中先于呼吸道过敏的发展。我们在旧秩序中的研究 门诺派社区表示，他们患特应性疾病和婴儿肠道微生物组的风险较低。 成分与罗切斯特婴儿不同。 队列管理和生物库核心将形成不同但 U19计划的补充项目1-3，“特应性早期开始的生物标志物”（BABE）。核心 我将跟踪并扩展一项正在进行的特应性皮炎风险极低的OOM新生儿纵向出生队列研究。 皮炎、食物过敏、枯草热和哮喘以及来自特应性家族的罗切斯特新生儿， 发展出这些特应性症状我们将检验婴儿肠道微生物组与 代谢途径和先天刺激促进了OOM中的耐受性和屏障功能。具体目标 的核心是目标1）样本和临床随访已招募的队列1婴儿，直至6岁 对于其他过敏性疾病，目标2）招募、采样和临床随访120名婴儿的类似队列2 对于特应性皮炎和食物过敏的发展，直到24个月的年龄，目的3）准备，储存和分享 项目1-3中使用的样本（脐带血和婴儿外周血、粪便、皮肤拭子和胶带），以评估 农业生活方式对儿童肠道微生物组，先天和适应性免疫组成以及皮肤屏障的影响 功能和微生物组组成。 这些研究旨在为儿童早期过敏性疾病的一级和二级预防提供新的策略。 疾病