Analytical Services Core

分析服务核心：项目摘要/摘要 分析服务核心（ASC）和两个子核心（脂质和氧化应激）的目的是提供 范德比尔特糖尿病研究中心（VDRC）的研究人员使用测定血浆和组织的分析方法 激素、细胞因子、氨基酸、脂质/脂蛋白、核苷酸、氧化应激标志物和GCMS 用于代谢通量分析的同位素富集和同位素异构体分布和质量的测定。 这些服务需要独特的仪器，并涉及严格的标准化程序。它既不是 在个别实验室建立这些测定法既不实际也不经济合理。巩固 将多项服务整合到一个核心实验室，为研究者提供高效、高质量、低成本的分析 由该领域的专家。所有服务都是量身定制的，以满足特定研究者的需求， 所遵循的方案可能会有所不同，这取决于研究者所需的分析和样本体积， 被分析。通过培训和教育，调查人员更加了解所提供的各种服务 以及这些服务如何解决特定的兴趣领域。最后，ASC的一个重要功能是 方法开发，这不仅增强了所提供的服务范围，而且最终还增加了 通过为研究人员提供扩大其研究计划的机会来提高效率。核心提供 用于样品分析的空间、设备和人员。调查人员支付服务费， 试剂、用品、按比例分配的设备服务合同和人员薪金的百分比。的 对于那些没有现成的湿实验室研究空间的研究人员来说，核心也是一种宝贵的资源， 人员的核心服务利用由经验丰富的研究人员在 一致的质量控制环境。ASC与其他VDRC核心密切合作， 操作程序，以便于将该技术转化为VDRC研究者在体内和体内进行的研究。 体外研究。调查人员依靠核心和次核心董事及其工作人员就如何收集 并处理在体内研究期间收集的样品。因此，ASC为VDRC提供基本服务 研究人员，使他们能够进行重要的和创新的糖尿病研究。

Analytical Services Core: Project Summary/Abstract The purpose of the Analytical Services Core (ASC) and two Subcores (Lipid and Oxidative Stress) is to provide Vanderbilt Diabetes Research Center (VDRC) investigators with assays that measure plasma and tissue hormones, cytokines, amino acids, lipids/lipoproteins, nucleotides, markers of oxidant stress, and GCMS determination of isotopic enrichment and isotopomer distribution and mass used for metabolic flux analysis. These services require unique instrumentation and involve rigorous standardization of procedures. It is neither practical nor economically reasonable to establish these assays in individual laboratories. The consolidation of services into one core laboratory provides investigators with efficient, high quality, low-cost analyses performed by experts in the field. All services are tailored to meet the needs of the specific investigator in that the exact protocol followed may differ depending on the analysis desired by the investigator and the sample volume to be analyzed. Through training and education, investigators become more aware of the various services offered and how these services might address specific areas of interest. Finally, an important function of the ASC is method development, which not only enhances the spectrum of services offered but ultimately also increases efficiency by providing researchers with the opportunity to expand their research programs. The core provides space, equipment, and personnel for sample analysis. Investigators pay a fee-for-service that covers the cost of reagents, supplies, pro-rated service contracts for equipment, and a percentage of personnel salary. The core also serves as a valuable resource for investigators without ready access to wet-lab research space and personnel. Core services utilize the latest instrumentation run by experienced research personnel in a consistent, quality-controlled environment. The ASC works closely with other VDRC cores to develop standard operating procedures to facilitate translation of this technology to VDRC investigators performing in vivo and in vitro studies. Investigators rely on the Core and Subcore directors and their staff for advice on how to collect and process samples collected during in vivo studies. Thus, the ASC provides essential services to VDRC investigators that allow them to perform important and innovative diabetes research.