Core B – Human Biospecimen and Advanced Sequencing Core
核心 B — 人类生物样本和高级测序核心
基本信息
- 批准号:10634587
- 负责人:
- 金额:$ 31.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-01 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAdipocytesAdipose tissueAutomobile DrivingBenignBiometryBloodBody Weight decreasedCachexiaCell NucleusCell SeparationCellsClinicalClinical DataComprehensive Cancer CenterConsentDNA Sequencing FacilityDataDedicationsDevelopmentDiagnosisDiseaseEnsureExhibitsFibroblastsGene ExpressionGene Expression ProfilingGoalsHistologicHumanImmune systemIndianaIndividualInfrastructureInterleukin-6KnowledgeMacrophageMalignant NeoplasmsMalignant neoplasm of pancreasMetabolicMolecularMorbidity - disease rateMusMuscleMuscular AtrophyNF-kappa BOperative Surgical ProceduresPancreatic Ductal AdenocarcinomaPathologyPatientsPeripheralPhenotypePreparationProcessProtocols documentationQuality of lifeReportingResearchResearch PersonnelResolutionResourcesRoleSTAT3 geneSamplingSequence AnalysisSignal TransductionSkeletal MuscleSpecimenTechnologyTestingTissue SampleTissuesTreatment-related toxicityTumor BiologyTumor PromotionUniversitiesWasting SyndromeWorkcancer cachexiaclinical translationeffective therapyhuman datahuman tissueimprovedmortalitymouse modelneoplastic cellnovelpancreatic cancer patientspreservationprogramsreceptorsample collectionsingle cell analysissingle nucleus RNA-sequencingsingle-cell RNA sequencingsuccesstranscriptome sequencingtranscriptomic profilingtranslational potentialtreatment responsetumortumor microenvironmentwasting
项目摘要
PROJECT SUMMARY: CORE B
The goal of this Program is to advance knowledge of pancreatic ductal adenocarcinoma (PDAC), a recalcitrant
cancer with one of the highest rates of cachexia. Project 1 focuses on PDAC-induced circulating factors, including
IL-6 and its soluble receptor (sIL6R), leading to wasting of the target tissues via activation of STAT3 and NF-kB
in adipocytes and myofibers. Project 2 investigates IL-6 and NF-kB in the skeletal muscle microenvironment in
PDAC cachexia, as well as NF-kB in the tumor microenvironment and its effects on macrophages. Project 3’s
studies probe interactions of IL-6, STAT3, and NF-kB among tumor cells, fibroblasts, and macrophages in the
tumor microenvironment. Together these studies will tackle both PDAC tumor biology and the metabolic havoc
it creates for the patient. Core B will provide the Projects with unprecedented potential to interrogate the PDAC
macroenvironment by providing 1) human clinical specimens to evaluate the translational potential of their
findings, and 2) the capacity to carry out transcriptome profiling at the single cell level using single cell and single
nucleus RNAseq in matched samples from patients with PDAC, and 3) dedicated support for advanced sequence
analysis. In doing so, this Core addresses barriers that have historically limited research in PDAC and cancer
cachexia. Very few studies have reported gene expression or histological endpoints in peripheral tissues in
human cancer cachexia and even fewer in PDAC cachexia, and none have attempted to match analyses across
tissues. As well, these pioneering studies at single cell resolution in the PDAC macroenvironment of mouse
models and humans, carried out by investigators at the forefront of these technologies and analytical capabilities
will enable the Projects to make groundbreaking discoveries while contributing novel, high resolution data to the
field. The specific aims are to 1) Collect, store and distribute clinically annotated PDAC cachexia
biospecimens. Clinical data and biospecimens will be collected from PDAC surgical patients at Indiana
University Simon Comprehensive Cancer Center (IUSCCC) and from patients from the Danish PACTO Trial for
centralized compliance, processing, and distribution. 2) Carry out a single nucleus (sn) RNAseq study of the
human PDAC cachexia macroenvironment for all Projects. Muscle and adipose samples from IUSM general
surgery patients with benign conditions and muscle, adipose and tumor samples from IUSCCC PDAC surgery
patients with and without moderate cachexia will be processed to make single nuclei preparations. Batched
samples will be subjected to snRNAseq to test specific hypotheses for each of Projects 1-3 and the overall
program. 3) Provide advanced analysis of single cell (sc) and snRNAseq studies for all Projects. Core B
will provide dedicated advanced analysis of the snRNAseq data from human specimens in Aim 2 for Projects 1-
3, and of sn or scRNAseq data from mice emanating from each of the Projects 1-3. Together we will identify
targetable vulnerabilities in PDAC to promote tumor killing while sparing host tissues to preserve function,
improve quality of life and response to therapy, and ultimately increase survival for patients with PDAC.
项目总结:核心b
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LEONIDAS G. KONIARIS其他文献
LEONIDAS G. KONIARIS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LEONIDAS G. KONIARIS', 18)}}的其他基金
Core B – Human Biospecimen and Advanced Sequencing Core
核心 B — 人类生物样本和高级测序核心
- 批准号:
10172473 - 财政年份:2021
- 资助金额:
$ 31.52万 - 项目类别:
Core B – Human Biospecimen and Advanced Sequencing Core
核心 B — 人类生物样本和高级测序核心
- 批准号:
10441216 - 财政年份:2021
- 资助金额:
$ 31.52万 - 项目类别:
相似国自然基金
支链氨基酸代谢紊乱调控“Adipocytes - Macrophages Crosstalk”诱发2型糖尿病脂肪组织功能和结构障碍的作用及机制
- 批准号:81970721
- 批准年份:2019
- 资助金额:55.0 万元
- 项目类别:面上项目
相似海外基金
Recruitment of brown adipocytes in visceral white adipose tissue by fibroblast growth factor 8b
成纤维细胞生长因子 8b 将棕色脂肪细胞募集到内脏白色脂肪组织中
- 批准号:
321208980 - 财政年份:2016
- 资助金额:
$ 31.52万 - 项目类别:
Research Grants
Enhancing Energy Expending Adipocytes in White Adipose Tissue
增强白色脂肪组织中的能量消耗脂肪细胞
- 批准号:
8827438 - 财政年份:2014
- 资助金额:
$ 31.52万 - 项目类别:
Induction of brown-like adipocytes in white adipose tissue by food-derived factors
食物源性因子在白色脂肪组织中诱导棕色样脂肪细胞
- 批准号:
26450168 - 财政年份:2014
- 资助金额:
$ 31.52万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
WAT-on-a-chip - Development of a micofluidic, microphysiologic in vitro adipose tissue model for high-throughput drug screening based on hiPSC-derived adipocytes.
WAT-on-a-chip - 开发微流体、微生理体外脂肪组织模型,用于基于 hiPSC 衍生脂肪细胞的高通量药物筛选。
- 批准号:
257256526 - 财政年份:2014
- 资助金额:
$ 31.52万 - 项目类别:
Research Fellowships
Enhancing Energy Expending Adipocytes in White Adipose Tissue
增强白色脂肪组织中的能量消耗脂肪细胞
- 批准号:
8828181 - 财政年份:2013
- 资助金额:
$ 31.52万 - 项目类别:
Enhancing Energy Expending Adipocytes in White Adipose Tissue
增强白色脂肪组织中的能量消耗脂肪细胞
- 批准号:
8520690 - 财政年份:2013
- 资助金额:
$ 31.52万 - 项目类别:
Enhancing Energy Expending Adipocytes in White Adipose Tissue
增强白色脂肪组织中的能量消耗脂肪细胞
- 批准号:
8629741 - 财政年份:2013
- 资助金额:
$ 31.52万 - 项目类别:
Effect of exercise training on formation of brite adipocytes within white adipose tissue
运动训练对白色脂肪组织内脂肪细胞形成的影响
- 批准号:
23700778 - 财政年份:2011
- 资助金额:
$ 31.52万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Investigation for the mechanisms of the emergence of brown adipocytes in white adipose tissue
白色脂肪组织中棕色脂肪细胞出现机制的研究
- 批准号:
21780261 - 财政年份:2009
- 资助金额:
$ 31.52万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
LOUISIANA COBRE: P1: INDUCE THERMOGENIC BROWN ADIPOCYTES IN WHITE ADIPOSE TISSUE
路易斯安那 COBRE:P1:在白色脂肪组织中诱导产热棕色脂肪细胞
- 批准号:
7610781 - 财政年份:2007
- 资助金额:
$ 31.52万 - 项目类别:














{{item.name}}会员




