The Influence of Vocal Loading Upon the Healing of Experimental Vocal Fold Injury

声带负荷对实验性声带损伤愈合的影响

• 批准号: 10674999
• 负责人: TOBIAS RIEDE
• 金额: $ 18.75万
• 依托单位: MIDWESTERN UNIVERSITY (GLENDALE AZ)
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10674999
• 关键词: 3-Dimensional; Acceleration; Acoustics; Affect; Air Movements; Anatomy; Animal Model; Behavior; Biomechanics; California; Cartilage; Characteristics; Cicatrix; Collagen; Communication impairment; Complex; Computer Models; Dependence; Development; Disease; Dose; Dysphonia; Elements; Epithelium; Female; Fiber; Frequencies; Genetic; Growth; Histology; Hoarseness; Hormones; Human; Hygiene; Individual; Injury; Investigation; Laboratories; Lamina Propria; Laryngeal Injury; Laryngeal cartilage; Larynx; Life; Maintenance; Measures; Modeling; Morphology; Movement; Mus; Noise; Occupational; Organ; Outcome; Output; Pathology; Pattern; Peromyscus; Phenotype; Pliability; Population; Procedures; Process; Production; Property; Public Health; Recovery; Research; Role; Shapes; Speech; Structure; Study models; Symptoms; Testing; Therapeutic Intervention; Thick; Time; Tissues; Variant; Voice; Work; X-Ray Computed Tomography; behavioral phenotyping; density; epithelial injury; experience; healing; in vitro Model; in vivo Model; male; microCT; mouse model; nerve supply; phenotypic biomarker; postnatal; postnatal development; soft tissue; therapeutic development; three-dimensional modeling; vibration; vocal cord; vocalization; wound healing

Project Summary The development and maintenance of a speaking and singing fundamental frequency, while also achieving vocal clarity throughout life, are important public health concerns. Symptoms of dysphonia are among the most common communication disorders. Currently there is no treatment to restore a native vocal fold lamina propria composition or laryngeal movements after injury. Research is limited because there is no in vivo model for larynx injuries that express a vocal behavior that can serve as a reliable phenotypic marker. In general, an animal model can benefit research if its behavior can serve as marker for the onset or the time-course of a pathology affecting a complex behavior. Specifically, the availability of an animal model that combines experimental and genetic accessibility with important similarities to human voice production could allow stronger inferences. Here we propose (1) to quantify key anatomical features of the vocal organ during postnatal developmental in a new model, and (2) to describe the time-course of wound healing after experimental vocal fold injury in a vocal dose- dependent fashion. Results will be integrated in a computational vocal fold model. Completion of this work will provide the field with a new mouse model for the study of vocal disease mechanisms and therapeutic development. It will also inform the role of vocal occupancy on the healing process of vocal folds.

项目概要 发展和维持说话和唱歌的基频，同时实现声音清晰度 贯穿一生，都是重要的公共卫生问题。发声困难的症状是最常见的沟通症状之一 失调。目前尚无治疗方法可以恢复天然声带固有层组成或喉部运动 受伤后。研究是有限的，因为没有喉部损伤的体内模型可以表达可以表达声音的行为。 作为可靠的表型标记。一般来说，如果动物模型的行为可以作为标记，那么它就可以有利于研究 用于影响复杂行为的病理的发生或时间进程。具体来说，动物的可用性 将实验和遗传可及性与人类声音产生的重要相似性相结合的模型可以 允许更有力的推论。在这里，我们建议（1）量化产后发声器官的关键解剖特征 新模型中的发育，以及（2）描述实验性声带损伤后伤口愈合的时间过程 声音剂量依赖性时尚。结果将被整合到计算声带模型中。完成这项工作 将为该领域提供一种新的小鼠模型，用于声乐疾病机制和治疗开发的研究。它 还将告知声带占据对声带愈合过程的作用。