权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanisms underlying recurrent bacterial vaginosis and HIV risk A Multidisciplinary approach

复发性细菌性阴道病和艾滋病毒风险的机制多学科方法

基本信息

批准号：
10675424
负责人：
MARIA LUISA ALCAIDE
金额：
$ 63.6万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10675424
关键词：
16S ribosomal RNA sequencing AIDS prevention Address Affect Age Anti-Retroviral Agents Antibiotic Therapy Antibiotics Automobile Driving Bacteria Bacterial Vaginosis Bacteriophages Behavioral Biological Biological Factors Cells Cities Coculture Techniques Communities DNA sequencing Development Drug usage Environment Epithelium Female Flow Cytometry Genetic Gram-Negative Anaerobic Bacteria HIV HIV Infections HIV risk Health Hygiene Immune Immune response Immunity Immunologics Impairment In Vitro Incidence Inflammation Inflammatory Inflammatory Response Intervention Irrigation Knowledge Longitudinal Studies Maintenance Mass Spectrum Analysis Measures Metabolism Metronidazole Mucosal Immune Responses Mutation Pharmaceutical Preparations Questionnaires Race Recurrence Regulation Risk Role Self Administration Sex Behavior Sexually Transmitted Diseases Shotguns Technology Treatment Efficacy Treatment Failure United States National Institutes of Health Vagina Vaginal Douching Viral Woman bacterial community bacterial metabolism cervicovaginal cohort cytokine drug metabolism effective therapy genital infection in vivo ineffective therapies interdisciplinary approach intraepithelial microbial microbial community microbiome microbiota prevent reproductive reproductive tract sociodemographic factors transcription factor transcriptome sequencing vaginal lactobacilli vaginal microbiome vaginal microbiota virome

项目摘要

Abstract Bacterial vaginosis (BV), the most common health condition affecting the female reproductive tract, significantly increases the risk of HIV and STI acquisition in women. BV is characterized by changes in the composition of the vaginal bacteria (microbiota), with a reduction in healthy vaginal Lactobacillus species and an increase in gram negative anaerobic bacteria. Women with BV have impaired female reproductive tract mucosal immune responses with increased HIV target cells, as well as decreased epithelial integrity; all which lead to increased HIV risk. Furthermore, a recent study demonstrated that BV-associated bacteria can directly hydrolyze drugs used for HIV prevention, setting up conditions that undermine treatment efficacy. Current antibiotic-based treatments for BV result in failure of treatment or recurrence in the majority of women. Behavioral (sexual behaviors and intravaginal hygiene practices such as intravaginal douching), bacterial factors (microbiome and virome), and host biological factors (mucosal immune responses and inflammation), all may underscore BV recurrence. In this study, we will assess the mechanisms underlying BV recurrence in a unique cohort of reproductive age women (18-45 years) with BV. Reproductive age women with BV will be provided BV treatment and followed longitudinally (one and six months post-BV treatment). Study aims are: 1) To evaluate the role of intravaginal hygiene practices on BV recurrence; 2) To evaluate the role of the vaginal microbiome and virome, and antibiotic metabolism by vaginal bacteria, on BV recurrence; and 3) To evaluate the role of host biological factors on BV recurrence. To our knowledge, this is the first study to mechanistically assess factors that underlie BV recurrence in vivo in a longitudinal study, and specifically, using a comprehensive, multidisciplinary approach (behavioral, microbiological, and immunological). We will use cutting edge technologies to measure these potential mechanisms relative to BV recurrence. Study results will be critical to develop targeted intervention strategies to prevent BV recurrence and thus, better protect women from HIV and STIs.

摘要