Metabolomics Shared Resource

项目摘要：代谢组学共享资源(MSR) 代谢组学共享资源正在进化，以满足丹·L的研究需求和使命 邓肯综合癌症中心(DLDCCC)。此共享资源(SR)提供高级质量 所需的光谱方法和技术、科学咨询和专家数据分析 高质量的全球和靶向代谢组学研究。MSR专门从事发现、识别、 生物分子的表征和量化，以及代谢通量分析，来自各种 生物标本，包括组织、细胞系和体液。MSR的主要基础设施包括5个 最适合于以下应用的高端质谱柱和前向液相色谱柱 涵盖作为服务开发的代谢分析的范围，包括Agilent 6490 Triple Quad 质谱仪，2台安捷伦6495三重四重质谱仪，一台安捷伦6550飞行时间质谱仪 一台带有Schimadzu Nexera UHPLC系统的SCIEX Triple TOF质谱仪，其数据- 脂类组学的独立收购。MSR拥有一支专门的质谱学专家团队， 在代谢组学方面有丰富的经验，并在细胞代谢和数据方面具有生物学专业知识 分析。代谢组学终于首次成为癌症中心的共享资源 与之竞争的CCSG在2015年的续签。从那时起，MSR支持了广泛的DLDCCC 7个计划中的6个计划和总共57个出版物的成员，其中许多代表高 影响期刊包括《自然》、《临床研究杂志》、《自然通讯》、《公共科学图书馆·遗传学》、 自然代谢、细胞报告、癌细胞、癌症研究和临床癌症研究。这是一个 动态SR设施，跟上了快速发展的技术和工具的步伐，一直是 通过开发和发布创新技术，在该领域处于领先地位。MSR已经发展出最先进的- ART技术作为核心支持服务，包括以下内容：(1)稳态靶向代谢组学 有能力对多达700种已知代谢物进行量化；(2)用于鉴定的非靶向代谢组学 最多1200个代谢物；(3)最多800个分子的脂质组学；(4)代谢通量分析，以监测 实验模型系统中主要代谢途径活性的变化。中建议的MSR 这一应用，可以弥合我们对癌症生物学和分子通路的理解的差距 使广泛的DLDCCC研究小组能够利用切割来探索新陈代谢在癌症中的作用 高质量、高效率、高精确度的尖端技术。

PROJECT SUMMARY: Metabolomics Shared Resource (MSR) The Metabolomics Shared Resource (MSR) is evolving to fulfill the research needs and mission of the Dan L Duncan Comprehensive Cancer Center (DLDCCC). This Shared Resource (SR) provides advanced mass spectrometry approaches and technologies, scientific consultation, and expert data analytics as is required for high quality global and targeted metabolomics studies. The MSR specializes in discovery, identification, characterization, and quantification of biomolecules, as well as metabolic flux analysis, from a variety of biological specimens including tissues, cell lines, and fluids. The main infrastructure of the MSR consists of 5 high-end mass spectrometry systems and upfront liquid chromatography columns that are most suitable for covering the range of metabolomic analyses developed as services including an Agilent 6490 Triple Quad Mass Spectrometer, 2 Agilent 6495 Triple Quad Mass Spectrometers, an Agilent 6550 Time of Flight Mass Spectrometer, and a SCIEX Triple TOF mass spectrometer with Schimadzu Nexera UHPLC-System with data- independent acquisition for lipidomics. The MSR has a dedicated team of experts in mass spectrometry with extensive experience in metabolomic profiling and biological expertise in cellular metabolism and data analysis. Metabolomics became a Shared Resource of the Cancer Center for the first time at the last competing renewal of the CCSG in 2015. Since then, the MSR has supported a broad range of DLDCCC members across 6 of the 7 Programs and a total of 57 publications, many representing major projects in high- impact journals including Nature, Journal of Clinical Investigations, Nature Communications, PLoS Genetics, Nature Metabolism, Cell Reports, Cancer Cell, Cancer Research, and Clinical Cancer Research. This is a dynamic SR facility that has kept pace with rapidly emerging technologies and instrumentation and has been a leader in the field by developing and publishing innovative technologies. The MSR has developed state-of-the- art technologies as core support services that includes the following: (1) Steady-state targeted metabolomics with a capacity for quantification of up to 700 known metabolites; (2) Untargeted metabolomics for identification of up to 1200 metabolites; (3) Lipidomics for up to 800 molecules and; (4) Metabolic flux analysis to monitor changes in activities of major metabolic pathways in experimental model systems. The MSR, as proposed in this application, can bridge the gap in our understanding of molecular pathways involved in cancer biology and enable a wide range of DLDCCC research groups to explore the role of metabolism in cancer utilizing cutting- edge technologies with quality, efficiency, and accuracy which would not be possible otherwise.