Role of vimentin in mammalian cell motility
波形蛋白在哺乳动物细胞运动中的作用
基本信息
- 批准号:10676985
- 负责人:
- 金额:$ 37.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-16 至 2026-07-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalActinsAddressAdhesionsCell CommunicationCell NucleusCell surfaceCellsCytoskeletonDiseaseDisease ProgressionEmbryonic DevelopmentEnvironmentExtracellular MatrixF-ActinFocal AdhesionsHealthHumanIntegrinsIntermediate Filament ProteinsIntermediate FilamentsMaintenanceMalignant NeoplasmsMammalian CellMechanicsMediatingMicrofluidicsMicrotubulesNeoplasm MetastasisNuclearPhysiologicalPlayPolymersPositioning AttributeProcessRoleStress FibersSurfaceTissuesVimentincell motilitycellular resiliencemigrationwound healing
项目摘要
ABSTRACT
Cell migration is essential to many fundamental processes, including embryonic development, wound repair,
and cancer metastasis. Central to this process is the cellular cytoskeleton comprised of three polymeric
networks: F-actin, microtubules, and intermediate filaments (IFs). Vimentin is an IF protein that is essential to
the mechanical resilience of cells and regulates cross-talk amongst the cytoskeleton, but its role in how cells
squeeze through small pores in tissues is poorly understood. We have shown that loss of vimentin enhances
cell motility through three-dimensional 3D micro-fluidic channels and protects the nucleus from damage during
migration. Vimentin is thought to play a distinct role in the transfer of forces from cell surface matrix adhesions
to the nuclear surface, but new evidence suggests that vimentin may also play a more active role in persistent
cell motility via its interactions with actin stress fiber formation and microtubule positioning. Still, the specific
mechanisms by which vimentin enables 3D migration through dense tissue remains unclear. This project
addresses the overarching question: how does vimentin influence cytoskeletal functions, adhesions with
the extracellular matrix, and cell-cell interactions to coordinate 3D cell migration? To address this
question, we will pursue three sub-projects. First, we will determine the effects of vimentin in cytoskeletal-
mediated 3D cell motility. Second, we will identify vimentin’s role in integrin expression and focal adhesion
activation, and third, we will identify the mechanisms by which vimentin mediates collective cell migration
through extracellular matrix networks. These studies will be conducted in both 2D and 3D settings to define
how changes in the cellular environment impact the critical determinants of cytoskeletal polymers in cell
motility. We expected that these projects will determine new functional roles of vimentin in cell migration, which
has important implications for understanding healthy tissue maintenance and diseases that progress by the
migration of cells through tissues.
摘要
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Dynamic remodeling of fiber networks with stiff inclusions under compressive loading.
- DOI:10.1016/j.actbio.2022.09.063
- 发表时间:2023-06
- 期刊:
- 影响因子:9.7
- 作者:Carroll B;Thanh MH;Patteson AE
- 通讯作者:Patteson AE
A Torsion-Based Rheometer for Measuring Viscoelastic Material Properties
用于测量粘弹性材料特性的基于扭转的流变仪
- DOI:10.35459/tbp.2020.000172
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Asp, Merrill;Jutzeler, Elise;Kochanowski, Jakub;Kerr, Katherine;Song, Dawei;Gupta, Sarthak;Carroll, Bobby;Patteson, Alison
- 通讯作者:Patteson, Alison
Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures.
- DOI:10.1016/j.jbc.2023.104963
- 发表时间:2023-08
- 期刊:
- 影响因子:4.8
- 作者:Bucki, Robert;Iwamoto, Daniel, V;Shi, Xuechen;Kerr, Katherine E.;Byfield, Fitzroy J.;Suprewicz, Lukasz;Sklodowski, Karol;Sutaria, Julian;Misiak, Pawe l;Wilczewska, Agnieszka Z.;Ramachandran, Sekar;Wolfe, Aaron;Thanh, Minh-Tri Ho;Whalen, Eli;Patteson, Alison E.;Janmey, Paul A.
- 通讯作者:Janmey, Paul A.
The molecular biophysics of extracellular vimentin and its role in pathogen-host interactions.
- DOI:10.1016/j.ceb.2023.102233
- 发表时间:2023-09
- 期刊:
- 影响因子:7.5
- 作者:Sepideh Parvanian;Leila S. Coelho-Rato;John E. Eriksson;Alison E. Patteson
- 通讯作者:Sepideh Parvanian;Leila S. Coelho-Rato;John E. Eriksson;Alison E. Patteson
How cells wrap around virus-like particles using extracellular filamentous protein structures.
细胞如何利用细胞外丝状蛋白结构包裹病毒样颗粒。
- DOI:10.1101/2023.01.30.526272
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Gupta,Sarthak;Santangelo,ChristianD;Patteson,AlisonE;Schwarz,JM
- 通讯作者:Schwarz,JM
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Alison Elise Patteson其他文献
Alison Elise Patteson的其他文献
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{{ truncateString('Alison Elise Patteson', 18)}}的其他基金
Role of vimentin in mammalian cell motility
波形蛋白在哺乳动物细胞运动中的作用
- 批准号:
10468232 - 财政年份:2021
- 资助金额:
$ 37.5万 - 项目类别:
Role of vimentin in mammalian cell motility
波形蛋白在哺乳动物细胞运动中的作用
- 批准号:
10582235 - 财政年份:2021
- 资助金额:
$ 37.5万 - 项目类别:
Role of vimentin in mammalian cell motility
波形蛋白在哺乳动物细胞运动中的作用
- 批准号:
10276323 - 财政年份:2021
- 资助金额:
$ 37.5万 - 项目类别:
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