Cell Preparation and Analysis Core

细胞制备和分析核心

• 批准号: 10677841
• 负责人: Diane S Krause
• 金额: $ 21.99万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10677841
• 关键词: Address; Area; Awareness; Bioinformatics; Budgets; CD34 gene; Categories; Cells; Clinical Trials; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Discipline; Emerging Technologies; Faculty; Funding; Gene Library; Genomics; Goals; Hematology; Hematopoiesis; Human; Individual; Institution; Laboratories; Laboratory Research; National Institute of Diabetes and Digestive and Kidney Diseases; Non-Malignant; Paper; Patients; Persons; Population; Preparation; Price; Protocols documentation; Publications; Reproducibility; Research; Research Personnel; Research Technics; Resources; Sampling; Services; Specimen; Techniques; Technology; Time; Tissue Banks; Training; Universities; Virus; cell preparation; cost; cost effective; design; experience; genome editing; innovation; interest; manufacture; member; prevent; stem; stem cells; success; synergism

Cell Preparation and Analysis Core ABSTRACT Members of the faculty of Yale University have a longstanding interest in non-malignant hematological research. This interest has further intensified in recent years, now encompassing multiple departments and a variety of disciplines. The Cell Preparation and Analysis Core (Core A) was initially established within the framework of Yale Cooperative Center of Excellence in Hematology (YCCEH) to address the needs and challenges of non-malignant hematology research laboratories both at Yale and in other institutions, by providing twelve services. Over the past four years, Core A has been successful in providing services and training, with an average of ~50 Yale and ~10 non-Yale users annually. Core A also has effectively trained and/or serviced 97 junior researchers, and contributed to the publication of 30 papers. With the success of Core A in the past funding cycle, we have also become increasingly aware of additional needs in non-malignant hematology research. We thus propose to continue the success of existing service offerings and to incorporate new services and training that answer to these additional demands from the hematology community. These 16 services and related training are designed to address challenges facing hematology laboratories, including (1) the relative lack of access to primary human cell populations and high commercial prices for the study of human hematopoiesis, (2) the wide spread interest in emerging cell preparation and analysis techniques which are difficult to establish and optimize, (3) the need to access primary patient samples, and (4) the necessity to train junior researchers to perfrom essential hematology research techniques with rigor and high reproducibility. Core A will continue to be directed by Dr. Diane Krause with the assistance of Associate Director Dr. Jun Lu. Additionally, Drs. Gary Kupfer and Manoj Pillai will be Key Core Faculty participating in Core A services. Drs. Krause, Lu, Kupfer and Pillai have extensive hematology research experience, which will be utilized to direct two research staff to provide effective services to YCCEH members and those in other NIDDK CCEH consortium. Junior investigators are particularly encouraged to use the Core services. Through centralization of key hematology techniques and establishment of a common platform for training, Core A will help individual laboratories to considerably increase research efficiency, reduce expense, and save valuable investigator time. The training aspect of the core will allow efficient dissemination of relevant techniques and prevent redundancy of such efforts in member laboratories at Yale and across the nation. By maintaining and providing exciting new emerging technologies, Core A fulfills the overall goal of YCCEH so that innovative technical advances are shared and positively leveraged for the advancement of non-malignant hematology research.

细胞制备和分析核心 抽象的 耶鲁大学学院的成员对非恶性血液学感兴趣 研究。近年来，这种兴趣进一步加剧了，现在包括多个部门和 各种学科。细胞制备和分析核心（核心A）最初是在内部建立的 耶鲁大学血液学卓越中心（YCCEH）的框架，以满足需求和 耶鲁大学和其他机构中非机密血液学研究实验室的挑战，通过提供 十二个服务。在过去的四年中，核心A已成功提供服务和培训，并 平均每年约50耶鲁大学和约10个非YALE用户。核心A还有效地训练和/或维修97 初级研究人员，并为出版30篇论文做出了贡献。在过去的资金中，核心A的成功 循环，我们也越来越意识到非恶性血液学研究的其他需求。我们 因此，建议继续现有服务产品的成功，并纳入新服务和培训 对血液学社区的这些额外要求的答复。这16个服务和相关培训 旨在应对血液学实验室面临的挑战，包括（1）相对缺乏访问权限 人类细胞的原发性细胞种群和人类造血研究的高商业价格，（2） 传播对新兴的细胞制备和分析技术的兴趣，这些技术难以建立和优化， （3）需要访问初级患者样本，（4）需要培训初级研究人员的必要性 基本血液学研究技术具有严格性和高可重现性。核心A将继续被指导 戴安娜·克劳斯（Diane Krause）博士在副主任Jun Lu博士的协助下。另外，博士。 Gary Kupfer和 Manoj Pillai将是参加核心A服务的关键核心教师。博士。 Krause，Lu，Kupfer和Pillai有 广泛的血液学研究经验，将用于指导两名研究人员提供有效 YCCEH成员和其他NIDDK CCEH财团的服务。初级调查人员特别是 鼓励使用核心服务。通过关键血液学技术和机构的集中化 核心A的常见培训平台将帮助个体实验室大大增加研究 效率，降低费用并节省宝贵的调查员时间。核心的训练方面将允许有效 传播相关技术并防止耶鲁大学成员实验室的此类努力的冗余 整个国家。通过维护和提供令人兴奋的新新兴技术，核心A可以实现整体 YCCEH的目标，以便共享创新的技术进步，并积极利用 非恶性血液学研究。