Cardiomyocyte bromodomain protein 4 (BRD4) in physiology and disease

心肌细胞溴结构域蛋白 4 (BRD4) 在生理学和疾病中的作用

• 批准号: 10681357
• 负责人: JOSEPH A HILL
• 金额: $ 61.06万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10681357
• 关键词: Acetylation; Biology; Bromodomain; Bromodomains and extra-terminal domain inhibitor; C-terminal; Cardiac; Cardiac Myocytes; Cardiovascular system; Cells; ChIP-seq; Chromatin; Chromatin Structure; Clinical; Coupled; Cues; Data; Development; Dilated Cardiomyopathy; Disease; Endogenous Factors; Engineering; Enhancers; Epigenetic Process; Event; Exons; Family; Family member; Fostering; Gene Expression; Gene Expression Profile; Genes; Genetic Transcription; Growth; HDAC1 gene; Heart; Heart Hypertrophy; Heart failure; Homeostasis; In Vitro; Ischemia; Knockout Mice; Literature; Mitochondria; Oncology; Outcome; Pathogenesis; Pathologic; Pathology; Pathway interactions; Phase; Phenotype; Physiology; Play; Positive Transcriptional Elongation Factor B; Post-Translational Protein Processing; Process; Protein Family; Protein Isoforms; Proteins; RNA Interference; Reader; Reperfusion Injury; Role; Small Interfering RNA; Stress; Therapeutic; Transcription Alteration; Transcriptional Regulation; Transgenic Mice; Variant; Ventricular; Work; cardioprotection; epigenetic therapy; heart function; histone modification; in vitro Model; in vivo; interest; knock-down; knockout animal; mouse model; pressure; response; small molecule; small molecule inhibitor; tool; transcriptome sequencing

Project Summary Together with the effects of modifier genes, sequence variants, endogenous factors, and environmental cues, strong evidence points to epigenetic events contributing to heart failure pathogenesis. Furthermore, epigenetic therapies have emerged already in oncology. Work from our lab and others has demonstrated robust cardioprotective effects of small molecule inhibitors of class 1 histone deacetylases (acetyl “erasers”) in both pressure-overload stress and ischemia/reperfusion injury. In this proposal, we will turn our focus to the so-called “readers“ of acetylation, the BRD proteins, specifically BRD4. Prior work in the literature has demonstrated that a small molecule inhibitor of BRD proteins blunts pathological cardiac remodeling, raising the tantalizing prospect of targeting this biology for therapeutic gain. Depletion of specific BRD isoforms in those studies implicated BRD4. Importantly, BRD4 encodes at least two isoforms in the heart (BRD-L and BRD-S) via alternative exon usage. The long isoform BRD4-L is unique in the BET family in that it contains an extended C terminus that includes a P-TEFb-interacting domain. BRD4 is known to regulate superenhancers, large clusters of transcriptional enhancers that drive expression of genes that define cell identity. Indeed, BRD4-L is required for super-enhancer function, with the C-terminal domain necessary for the phase-shift occurring with formation of super-enhancer clusters. The short isoform BRD4-S lacks this C terminus. Beyond these structural differences, it is clear in the context of oncology and other domains that these BRD4 isoforms have distinct – indeed antagonistic – functions, a fact which complicates the interpretation of existing data in the cardiovascular space. Presently, nothing is known about the role of these BRD4 isoforms in the heart. Our preliminary data, using a cardiomyocyte-specific BRD4 knockout mouse, show that loss of BRD4 results in a rapid decline in ventricular contractile function and dilated cardiomyopathy. Our preliminary in vitro studies and in vivo studies targeting specific BRD4 isoforms suggests that BRD4-L is not required for cardiac hypertrophy, but rather, the hypertrophic response is dependent on BRD4-S. Here, we propose work based on the over-riding hypothesis that specific isoforms of BRD4 play distinct roles in cardiomyocyte physiology and pathology. Work to unveil isoform-specific functions will be a major step toward precision targeting of this biology in disease therapy. Already, we have developed unique tools to directly assess the roles of these isoforms both in vitro and in vivo.

项目总结

项目成果

Xbp1s-FoxO1 axis governs lipid accumulation and contractile performance in heart failure with preserved ejection fraction.

• DOI: 10.1038/s41467-021-21931-9
• 发表时间: 2021-03-16
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Schiattarella GG;Altamirano F;Kim SY;Tong D;Ferdous A;Piristine H;Dasgupta S;Wang X;French KM;Villalobos E;Spurgin SB;Waldman M;Jiang N;May HI;Hill TM;Luo Y;Yoo H;Zaha VG;Lavandero S;Gillette TG;Hill JA
• 通讯作者: Hill JA

Single-cell transcriptional landscape of long non-coding RNAs orchestrating mouse heart development.

• DOI: 10.1038/s41419-023-06296-9
• 发表时间: 2023-12-18
• 期刊: CELL DEATH & DISEASE
• 影响因子: 9
• 作者: Ramos, Thais A. R.;Urquiza-Zurich, Sebastian;Kim, Soo Young;Gillette, Thomas G.;Hill, Joseph A.;Lavandero, Sergio;do Rego, Thais G.;Maracaja-Coutinho, Vinicius
• 通讯作者: Maracaja-Coutinho, Vinicius

Fli1 Promotes Vascular Morphogenesis by Regulating Endothelial Potential of Multipotent Myogenic Progenitors.

• DOI: 10.1161/circresaha.121.318986
• 发表时间: 2021-10-29
• 期刊: Circulation research
• 影响因子: 20.1
• 作者: Ferdous A;Singh S;Luo Y;Abedin MJ;Jiang N;Perry CE;Evers BM;Gillette TG;Kyba M;Trojanowska M;Hill JA
• 通讯作者: Hill JA