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Developing Methanosarcina spp. as a model system to study cytochromes c and their role in archaeal methane metabolism

正在开发甲烷八叠球菌属。

基本信息

批准号：
10679362
负责人：
Dinesh Gupta
金额：
$ 6.95万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-30 至 2025-09-29
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10679362
关键词：
Anaerobic Bacteria Archaea Atmosphere Binding Biochemical Biogenesis Biological Models Biotechnology CRISPR/Cas technology Coculture Techniques Complex Consumption Coupled Development Electron Transport Electrons Engineering Environment Escherichia coli Evaluation Ferredoxin Gene Expression Genes Genetic Genetic Models Genome Genomics Goals Growth Health Heme Human In Vitro Knowledge Mediating Membrane Metabolic Methane Methane Metabolism Pathway Methanobacteria Methanosarcina Methanosarcina barkeri Modeling Molecular NADH Nature Nitrogen Fixation Operon Organism Oxidation-Reduction Pathway interactions Physiological Play Postdoctoral Fellow Process Production Proteins Reaction Research Rhodobacter Role Source Symbiosis System Techniques biochemical tools citrate carrier climate change climate crisis cofactor cytochrome c genome editing greenhouse gases improved in vivo insight interest methanophenazine microorganism oxidation reconstitution sodium ion success sulfate reducing bacteria technology development tool

项目摘要

Project Summary/Abstract Cytochromes c are crucial in methane-metabolizing archaea for the production and consumption of methane coupled to growth and energy conservation. Overall, methane-metabolizing archaea mediate the net flux of methane released into the atmosphere and thus, significantly impact the global methane cycle and climate change. Based on genomic studies, Cytochromes c have been hypothesized to play an important role in methane metabolism however the underlying molecular mechanisms remain elusive, primarily due to the absence of a well-developed genetic model system. Even attempts to study archaeal cytochrome c using an alternative approach such as heterologous expression in well-established bacterial systems have not proven successful so far. The main focus of the proposed research is to develop a genetically tractable methanogenic archaeon, Methanosarcina acetivorans, as a platform to functionally characterize archaeal cytochrome c and gain physiological insights into the role of cytochrome c in methane metabolism across different archaeal species. Cytochromes c are ubiquitous electron transfer proteins that require a covalent attachment to its heme co-factor, a process called cytochrome c biogenesis. Coordination between the cytochrome c biogenesis pathway and the cytochrome c of interest is critical for the successful production of a functional cytochrome c in a heterologous host. Using genetic and biochemical tools, I have recently characterized the cytochrome c biogenesis pathway in the model methanogenic archaeon, Methanosarcina acetivorans. Using this knowledge, the project aims to develop M. acetivorans as a genetic chassis to produce and functionally characterize archaeal cytochrome c from diverse archaeal species. Aim1 of the project will functionally characterize the crucial cytochromes c belonging to methane-producing archaea or methanogens, and Aim2 will study these proteins from methane-consuming archaea using both in vitro and in vivo analyses. This research will improve our understanding of methane metabolism and lead to the development of an archaeal host to study cytochrome c proteins from archaea. Ultimately, the knowledge garnered from these studies can be used to develop sustainable solutions for the global climate crisis and mitigate its harmful impacts on human health.

项目总结/文摘