Impact of HIV, oral microbiome and mycobiome on oral HPV persistence
HIV、口腔微生物组和真菌组对口腔 HPV 持久性的影响
基本信息
- 批准号:10683323
- 负责人:
- 金额:$ 82.06万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2027-08-31
- 项目状态:未结题
- 来源:
- 关键词:16S ribosomal RNA sequencingActinomycesActivities of Daily LivingAddressAffectAlcohol consumptionBacteriaBacterial InfectionsBiological AssayBiological MarkersCD4 Lymphocyte CountCandidaCohort StudiesCommunitiesCouplingCutaneousDataDentalDetectionDiseaseEnrollmentEthnic OriginEtiologyEvaluationExhibitsFemaleFrequenciesGenesHIVHIV InfectionsHPV-High RiskHead and Neck CancerHuman Papilloma Virus VaccinationHuman Papilloma Virus-Related Malignant NeoplasmHuman PapillomavirusHuman papillomavirus 16ImmuneImmune responseImmune systemImmunityIncidenceIndividualInfectionInflammationLesionLongitudinal StudiesMalasseziaMalignant NeoplasmsMeasuresMediationMetagenomicsMethodsMorbidity - disease rateMouth DiseasesMycosesNatural HistoryOncogenicOralOral CharactersOral cavityOral healthOrganismOutcomePapillomaParticipantPathway interactionsPatientsPersonsPorphyromonasPredispositionPrevalencePrevotellaProtein AnalysisPublic HealthRNARaceResearch DesignRibosomal RNARiskRisk FactorsSalivaSamplingSex BehaviorShotgunsSmokingSmoking BehaviorSmoking HistorySpecimenStructureTaxonomyTimeTobacco smoking behaviorTooth structureUnited StatesVariantViral Load resultVirulence FactorsWorkXerostomiaantiretroviral therapycancer riskcohortcytokinefungushigh riskinnovationmalemalignant mouth neoplasmmalignant oropharynx neoplasmmarijuana usemetagenomic sequencingmicrobialmicrobiomemycobiomeneoplasticnext generation sequencingnoveloral HPVoral HPV infectionoral lesionoral microbiomeoral wartpopulation basedresponsesaliva sampletherapy adherencetherapy durationvirome
项目摘要
ABSTRACT
Oral human papillomavirus (HPV) infections and HPV-associated papillomas and cancers continue to rise,
especially in people living with HIV (PLWH). Specifically, rates of HPV-associated oral and oropharyngeal
cancer are increasing at an alarming rate. Using large population-based longitudinal studies, our work
indicated an increased risk of oral lesions and head and neck cancers with not only HPV16, but unexpectedly
cutaneous beta and gamma HPVs as well. Studying risk factors, we identified an interaction between smoking
and HIV serostatus with increased prevalence of oral high-risk alpha and beta-HPV detection. In addition to
oral HPV, HIV affects the composition of the oral microbiome, which has also been associated with increased
susceptibility to oral HPV infection, persistence, and progression to HPV-driven cancer. Taken together, these
findings indicate a continuing public health concern of HPV-related oral diseases for PLWH. The proposed
study will leverage repeat oral saliva samples collected from N=2046 male and female PLWH, and N=1334
HIV[-] individuals enrolled in the MACS-WIHS Combined Cohort Study (MWCSS). Specifically, we will
investigate the associations between the oral microbiome and mycobiome and risk of acquisition and
persistence of high-risk alpha, beta, and gamma oral HPV. The scientific premise of the study is that
alterations in the oral microbiome / mycobiome resulting from HIV and smoking influence oral HPV natural
history. We will use novel sequencing methods developed by our team, including next generation sequencing
and metagenomics, to profile the HPV virome (alpha, beta, and gamma HPV), oral bacterial (16S rRNA) and
fungal communities (using a recently developed ITS1 assay with increased sensitivity for Candida developed in
the Burk lab), functional gene capacity (determined from shotgun metagenomic sequencing), and cytokine
profiles (derived from oral saliva protein analyses). Differences in the oral microbiome / mycobiome between
PLWH and HIV[-] individuals will be assessed, as well as markers of HIV disease and smoking, with respect to
oral HPV persistence. Taking advantage of serial samples collected in MWCCS participants, we will
characterize oral microbiome / mycobiome changes over time in response to CD4 count and HIV RNA level,
smoking, and the oral microbiome / mycobiome to address the following specific aims: 1) Determine the
combined effects of HIV and smoking on risk of oral HPV persistence; 2) Study the associations between the
oral microbiome / mycobiome and risk of oral HPV persistence; 3) Characterize the microbial function and oral
inflammation/immune pathways associated with risk of oral HPV persistence. We hypothesize that the oral
microbiome and mycobiome influenced by HIV and smoking exhibit significantly higher proportions of virulence
factors, which in turn dysregulate the oral barrier immunity and integrity, facilitating persistence of oral high-risk
HPV types associated with increased risks for oral and oropharyngeal cancer.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert D Burk其他文献
1296 FETAL ORIGIN OF MATERNAL SERUM ALPHA-FETOPROTEIN (AFP) DEMONSTRATED BY DNA-RNA HYBRIDIZATION
通过 DNA-RNA 杂交技术证明母体血清甲胎蛋白(AFP)的胎儿起源
- DOI:
10.1203/00006450-198504000-01320 - 发表时间:
1985-04-01 - 期刊:
- 影响因子:3.100
- 作者:
R Gordon Huteheon;Harold M Nltowsky;Sachiko Nakagawa;Robert D Burk - 通讯作者:
Robert D Burk
Oral HPV associated with differences in oral microbiota beta diversity and microbiota abundance.
口腔 HPV 与口腔微生物群 β 多样性和微生物群丰度的差异相关。
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:6.4
- 作者:
Yuehan Zhang;G. D’Souza;Carole Fakhry;Elaine O Bigelow;Mykhaylo Usyk;Robert D Burk;Ni Zhao - 通讯作者:
Ni Zhao
Association of meal timing with adiposity measures and gut microbiome characteristics in a cohort study: the Hispanic Community Health Study/Study of Latinos
一项队列研究中进餐时间与肥胖测量指标和肠道微生物组特征的关联:西班牙裔社区健康研究/拉丁裔研究
- DOI:
10.1016/j.ajcnut.2025.04.003 - 发表时间:
2025-06-01 - 期刊:
- 影响因子:6.900
- 作者:
Sarah K Alver;Brandilyn A Peters;Yasmin Mossavar-Rahmani;Qibin Qi;Amanda C McClain;Linda Van Horn;Robert D Burk;Robert C Kaplan - 通讯作者:
Robert C Kaplan
Robert D Burk的其他文献
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{{ truncateString('Robert D Burk', 18)}}的其他基金
Multi-omic signatures of gut dysbiosis and cardiovascular comorbidities associated with HIV infection
与 HIV 感染相关的肠道菌群失调和心血管合并症的多组学特征
- 批准号:
10762411 - 财政年份:2023
- 资助金额:
$ 82.06万 - 项目类别:
Epidemiology of diet, metabolism and non-alcoholic fatty liver disease in Hispanic/Latino adults
西班牙裔/拉丁裔成人饮食、代谢和非酒精性脂肪肝的流行病学
- 批准号:
10735454 - 财政年份:2023
- 资助金额:
$ 82.06万 - 项目类别:
Impact of HIV, oral microbiome and mycobiome on oral HPV persistence
HIV、口腔微生物组和真菌组对口腔 HPV 持久性的影响
- 批准号:
10534011 - 财政年份:2022
- 资助金额:
$ 82.06万 - 项目类别:
Investigations Into The Molecular Pathogenesis Of Cervical Glandular Neoplasias
宫颈腺瘤的分子发病机制研究
- 批准号:
10208823 - 财政年份:2020
- 资助金额:
$ 82.06万 - 项目类别:
Investigations Into The Molecular Pathogenesis Of Cervical Glandular Neoplasias
宫颈腺瘤的分子发病机制研究
- 批准号:
10652422 - 财政年份:2020
- 资助金额:
$ 82.06万 - 项目类别:
Investigations Into The Molecular Pathogenesis Of Cervical Glandular Neoplasias
宫颈腺瘤的分子发病机制研究
- 批准号:
9897227 - 财政年份:2020
- 资助金额:
$ 82.06万 - 项目类别:
Investigations Into The Molecular Pathogenesis Of Cervical Glandular Neoplasias
宫颈腺瘤的分子发病机制研究
- 批准号:
10443811 - 财政年份:2020
- 资助金额:
$ 82.06万 - 项目类别:
Next Generation of HPV and Cervical Cancer Research in HIV+ Women
HIV 女性中的下一代 HPV 和宫颈癌研究
- 批准号:
10203871 - 财政年份:2018
- 资助金额:
$ 82.06万 - 项目类别:
Next Generation of HPV and Cervical Cancer Research in HIV+ Women
HIV 女性中的下一代 HPV 和宫颈癌研究
- 批准号:
10440387 - 财政年份:2018
- 资助金额:
$ 82.06万 - 项目类别:
Epidemiology of the gut microbiome, prediabetes and diabetes in Latinos
拉丁裔肠道微生物组、糖尿病前期和糖尿病的流行病学
- 批准号:
9194787 - 财政年份:2016
- 资助金额:
$ 82.06万 - 项目类别:
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