Beta & Gamma Oral HPV infection in HIV positive individuals

贝塔

• 批准号: 9336891
• 负责人: Robert D Burk
• 金额: $ 13.39万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9336891
• 关键词: Adult; Alcohol consumption; Biopsy Specimen; Chronic; Clinical Data; Collaborations; DNA; DNA analysis; Data; Dental Hygiene; Detection; Development; Diagnosis; Disease; Epidemiology; Etiology; Failure; Funding; HIV; HIV Infections; HIV Seropositivity; Head and Neck Cancer; Head and Neck Neoplasms; Head and Neck Squamous Cell Carcinoma; Head and neck structure; Human Papillomavirus; Human papilloma virus infection; Human papillomavirus 16; Immune system; Immunosuppression; Incidence; Individual; Infection; Malignant Neoplasms; Malignant neoplasm of larynx; Michigan; Mouth Carcinoma; National Institute of Dental and Craniofacial Research; Natural History; Neoplasms; Opportunistic Infections; Oral; Oral Examination; Oral cavity; Participant; Patients; Population; Predisposition; RNA; RNA analysis; Recording of previous events; Research; Risk; Risk Behaviors; Risk Factors; Saliva; Sampling; Skin; Surveys; Testing; Time; Tobacco smoking; Tobacco use; Transcript; Universities; Viral; Virus Activation; Visit; base; cancer risk; carcinogenicity; clinical infrastructure; high risk; high risk population; malignant mouth neoplasm; malignant oropharynx neoplasm; next generation sequencing; oral infection; oral lesion; prospective

SUMMARY:   Infection with HIV increases susceptibility to opportunistic infections and cancer. The risk of oral and oropharyngeal carcinomas is increased in HIV-seropositive patients reflecting a higher rate of oral human papillomavirus (HPV) infection. Evidence from multiple studies indicates that the oral cavity contains a plethora of HPV species including beta and gamma-HPV types, previously associated with skin neoplasias. We recently a l s o found that detection of beta and gamma-HPV in the oral cavity was associated with increased risks of incident oral and laryngeal cancers. This suggests an etiologic association for beta and gamma- HPV types in the oral cavity, especially among HIV-infected populations who are at increased risk of developing a variety of virally induced head and neck tumors. However, to date, very little is known about the natural history of oral infection with beta and gamma-HPV types in either HIV-positive or HIV-negative populations. We will leverage the clinical infrastructure from an existing NIDCR funded study, and a collaboration with the University of Michigan Head and Neck SPORE HIV consortium supplement expanding the NIDCR project, to test repeated oral rinse, saliva and brush (DNA and RNA) samples collected from 660 HIV-positive and HIV-negative patients for multiple beta and gamma-HPV types by next-generation sequencing. Study participants are followed prospectively for a period of up to 3 years, and complete detailed epidemiologic surveys consisting of questions related to tobacco smoking, alcohol consumption and sexual practices. Clinical data related to control of HIV infection, oral lesions and history of other chronic infections are collected. Using this longitudinal data, we propose to: 1) assess and compare HPV type distribution and viral activation (expression) for beta- and gamma-HPV types in the oral cavity among HIV- positive and HIV-negative individuals, and 2) determine and compare the incidence and persistence of, and risk factors for, beta- and gamma-HPV infections in the oral cavity among HIV-positive and HIV-negative individuals. At the completion of this project, we will have precise estimates of the incidence and persistence of oral beta- and gamma-HPV infections, and identified risk factors for incident and persistent infection in HIV- positive and HIV-negative individuals. In addition, this study will be important for planning of larger collaborative studies, such as through the Head and Neck Inter-SPORE consortium, to assess the natural history of beta and gamma-HPV and their association with oral cancer risk in HIV-infected populations.

总结：   艾滋病毒感染增加了对机会性感染和癌症的易感性。口腔和 口咽癌在HIV血清阳性患者中增加，反映了口腔人类癌的高发病率。 乳头瘤病毒（HPV）感染。多项研究的证据表明，口腔中含有过多的 包括β和γ-HPV类型的HPV种类，以前与皮肤瘤形成有关。我们 最近，我们发现口腔中β和γ-HPV的检测与增加的 发生口腔癌和喉癌的风险。这表明β和γ的病因学联系- 口腔中的HPV类型，特别是在HIV感染人群中， 发展成各种病毒诱导的头颈部肿瘤。然而，到目前为止，人们对它知之甚少。 HIV阳性或HIV阴性患者的β和γ-HPV型口腔感染自然史 人口。我们将利用现有NIDCR资助研究的临床基础设施， 与密歇根大学头颈部孢子艾滋病联盟合作， NIDCR项目，测试从660个重复的口腔冲洗，唾液和刷（DNA和RNA）样本收集 按下一代分列的多种β和γ-HPV类型的艾滋病毒阳性和艾滋病毒阴性患者 测序对研究参与者进行长达3年的前瞻性随访，并完成 详细的流行病学调查，包括与吸烟、饮酒和 性行为与HIV感染控制、口腔病变和其他慢性病史相关的临床数据 感染被收集。利用这些纵向数据，我们建议：1）评估和比较HPV类型 HIV感染者口腔中β和γ型HPV的分布和病毒活化（表达）， 阳性和HIV阴性个体，以及2）确定和比较的发病率和持续性，和风险 HIV阳性和HIV阴性患者口腔中β和γ HPV感染的因素 个体在该项目完成时，我们将对以下情况的发生率和持续性作出精确估计： 口腔β-和γ-HPV感染，并确定了艾滋病毒偶发和持续感染的风险因素， 艾滋病毒阳性和阴性个体。此外，这项研究将是重要的规划， 合作研究，如通过头颈部Inter-SPORE联盟，以评估自然 HIV感染人群中β和γ-HPV病史及其与口腔癌风险的相关性