Integrated Structural Biology Core

综合结构生物学核心

• 批准号: 10684916
• 负责人: Stephen R Sprang
• 金额: $ 45.36万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-10 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10684916
• 关键词: Biochemistry; Biological Process; Biophysics; Cellular biology; Centers of Research Excellence; Charge; Client; Client satisfaction; Collaborations; Complex; Core Facility; Cryoelectron Microscopy; Crystallography; Ensure; Evaluation; Faculty; Fee-for-Service Plans; Funding; Funding Opportunities; Future; Genes; Goals; Grant; Institution; Laboratories; Ligand Binding; Measurement; Methodology; Mission; Molecular Biology; Molecular Structure; Monitor; Montana; Outcome; Performance; Phase; Pilot Projects; Policies; Process; Productivity; Property; Proteins; Publications; Reporting; Research; Research Personnel; Resources; Self-Examination; Services; Standardization; Structural Biochemistry; Structure; Surface Plasmon Resonance; Surveys; Technical Expertise; Technology; Thermodynamics; Training; Universities; Validation; X-Ray Crystallography; analytical method; analytical tool; design; instrumentation; light scattering; protein expression; protein purification; research faculty; service programs; small molecule; structural biology; student training; tool

The Integrated Structural Biology Core (ISBC) facility of the Center for Biomolecular Structure and Dynamics (CBSD) provides technology and expertise to assist University of Montana (UM) faculty to elucidate biological processes using the analytical methods of mechanistic and structural biochemistry. Services provided by the Core include a full suite of gene-to-structure technologies: protein expression and purification, structure determination by X-ray crystallography and cryo-Electron Microscopy (cryo-EM). The ISBC has the capacity to conduct a variety of biophysical and analytical measurements of protein thermodynamic and ligand-binding properties such as surface plasmon resonance and multi-angle light scattering, and supports NMR facilities at UM for structural analysis of macromolecular structure and dynamics, and structural validation of small organic molecules. The ISBC has also become a facility for small molecule X-ray crystallography for Montana and the surrounding Rocky Mountain region, which include three IDeA States. During Phase III of COBRE support the ISBC seeks: 1) to remain at the methodological forefront of structural biology by, among other activities, developing capacity for cryo-EM services in collaboration with partners at Montana State University; 2) to maintain strong and ongoing interactions with other core facilities within CBSD and other COBRE Core facilities at UM to provide a suite of complementary experimental and analytical tools to solve complex problems in molecular and cell biology; 3) optimize ISBC reporting processes to effectively track services provided to clients, evaluate scientific outcomes and impact, and monitor its financial status. The ISBC will employ evaluation/survey tools to ensure client satisfaction, identify emerging needs and define clear and transparent mechanisms to prioritize projects. The ISBC will continue to pursue its training mission to disseminate technologies to client laboratories. Lastly, the ISBC will implement policies to insure its sustainability beyond Phase III by maintaining a robust fee-for-service program, leveraging institutional support and pursuing grant-funded collaborations with UM investigators and external users.

生物分子结构和动力学中心的综合结构生物学核心（ISBC）设施 （CBSD）提供技术和专业知识，以协助蒙大拿大学（UM）教师阐明生物 使用机械和结构生物化学的分析方法的过程。提供的服务 核心包括一整套基因到结构技术：蛋白质表达和纯化、结构 通过X射线晶体学和低温电子显微镜（cryo-EM）测定。ISBC有能力 进行蛋白质热力学和配体结合的各种生物物理和分析测量 性能，如表面等离子体共振和多角度光散射，并支持NMR设施， UM用于大分子结构和动力学的结构分析，以及小分子有机物的结构验证 分子。ISBC也成为蒙大拿州和加利福尼亚州的小分子X射线晶体学设施。 落基山脉地区，其中包括三个IDEA州。在COBRE第三阶段， ISBC寻求：1）通过其他活动，保持结构生物学方法的前沿， 与蒙大拿州立大学的合作伙伴合作，发展低温EM服务能力; 2） 与CBSD和其他COBRE核心内的其他核心设施保持强有力的持续互动 设施在UM提供一套互补的实验和分析工具，以解决复杂的 分子和细胞生物学问题; 3）优化ISBC报告流程，以有效跟踪服务 它向客户提供科学数据，评估科学成果和影响，并监测其财务状况。ISBC将 采用评价/调查工具，确保客户满意，查明新出现的需求， 建立透明的机制，确定项目的优先次序。ISBC将继续履行其培训使命， 向客户实验室传播技术。最后，ISBC将实施政策， 通过保持强大的按服务收费计划，利用机构支持， 并寻求与UM调查人员和外部用户的赠款资助合作。