CENTER FOR BIOMOLECULAR STRUCTURE AND DYNAMICS

生物分子结构和动力学中心

• 批准号: 8359561
• 负责人: Stephen R Sprang
• 金额: $ 186.59万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359561
• 关键词: Arenavirus; Binding Sites; Biochemistry; Biological Sciences; Biology; Biophysics; Catabolism; Cells; Centers of Research Excellence; Chemicals; Chemistry; Collaborations; Faculty; Foundations; Funding; Glycoproteins; Goals; Grant; Heteronuclear NMR; Homology Modeling; Interdisciplinary Study; Junin virus; Ligands; Membrane Fusion; Mentors; Methods; Molecular; Molecular Computations; Molecular Structure; Montana; Multi-Drug Resistance; National Center for Research Resources; Opioid Receptor; Perception; Pharmacologic Substance; Physiology; Plant Roots; Principal Investigator; Recruitment Activity; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resources; Science; Self Assessment; Signal Transduction; Source; Spectrum Analysis; Structure; Theoretical Biology; Tryptophan; United States National Institutes of Health; Universities; Vision; X ray diffraction analysis; X-Ray Diffraction; base; computational chemistry; cost; design; enzyme mechanism; infrastructure development; innovation; interest; member; multidisciplinary; programs; research facility; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): The Center for Biomolecular Structure and Dynamics (CBSD) at the University of Montana (UM) is rooted in structural biology but multidisciplinary in scope. The rationale that underlies this program is based on the perception that effective biomedical innovation is driven from an understanding of macromolecular structure, which in turn, must be grounded on principles of experimental and theoretical chemistry. CBSD faculty are drawn from Department of Chemistry & Biochemistry, the Division of Biological Science and the Department of Biomedical and Pharmaceutical Sciences. Among this group are CBSD faculty with expertise in spectroscopy, structural biology, biochemistry and mechanistic and theoretical chemistry. We build on a strong foundation of sustained institutional support. Associated with the CBSD is a newly established interdisciplinary graduate program in Biochemistry and Biophysics. The goal of the proposed COBRE is to integrate investigators with research interests in cell and molecular physiology, mechanistic biochemistry, chemical biology, and theoretical and computational chemistry, into an interactive and collaborative network. With the creation of a CBSD COBRE, it is our goal to give structure to this vision by implementing the four aims of this proposal: Aim 1. Provide research support for five interdisciplinary research projects within the COBRE theme. These projects use theoretical and physical chemical methods to elucidate the catalytic mechanisms of enzymes involved in signal transduction and tryptophan catabolism. Structure-based homology modeling and chemical biology approaches will be used to design new ligands for the opioid receptor and to characterize the binding sites of the multidrug resistance transporter. Heteronuclear NMR experiments will be used to elucidate the structures of critical domains in the Junin arenavirus membrane fusion glycoprotein. Aim 2 shall be to recruit two new faculty members to CBSD-affiliated departments to build on existing strengths and introduce new expertise within the CBSD. Aim 3 will support collaborative activities involving CBSD faculty by sustaining of critical core research facilities in Macromolecular X-ray diffraction, Macromolecular NMR, BioSpectroscopy and Molecular Computation. Aim 4 will establish a supportive program to sustain an intellectually vibrant and cohesive CBSD that promotes infrastructure development, mentoring, scientific exchange and collaboration, with a robust advisory and self-assessment structure.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 描述（由申请人提供）：蒙大拿大学（UM）的生物分子结构和动力学中心（CBSD）植根于结构生物学，但范围涉及多学科。该计划的基本原理是基于有效的生物医学创新是从对大分子结构的理解驱动的，而这反过来又必须基于实验和理论化学的原则。CBSD的教师来自化学与生物化学系，生物科学系和生物医学与制药科学系。其中包括CBSD教师在光谱学，结构生物学，生物化学和机械和理论化学方面的专业知识。我们建立在持续的机构支持的坚实基础上。与CBSD相关的是一个新成立的生物化学和生物物理学跨学科研究生课程。拟议的COBRE的目标是将研究人员与细胞和分子生理学，机械生物化学，化学生物学，理论和计算化学的研究兴趣整合到一个互动和协作的网络中。随着CBSD COBRE的创建，我们的目标是通过实现本提案的四个目标来实现这一愿景：目标1。为COBRE主题内的五个跨学科研究项目提供研究支持。这些项目使用理论和物理化学方法来阐明参与信号转导和色氨酸催化的酶的催化机制。基于结构的同源性建模和化学生物学方法将用于设计阿片受体的新配体，并表征多药耐药转运蛋白的结合位点。异源NMR实验将用于阐明朱宁沙粒病毒膜融合糖蛋白中关键结构域的结构。目标2是为CBSD附属部门招聘两名新教师，以加强现有优势，并在CBSD内引入新的专业知识。目标3将通过维持高分子X射线衍射、高分子核磁共振、生物光谱学和分子计算领域的关键核心研究设施，支持涉及CBSD教师的合作活动。目标4将建立一个支持性计划，以维持一个充满活力和凝聚力的CBSD，促进基础设施建设，指导，科学交流和合作，并建立一个强大的咨询和自我评估结构。