Organ Specific Project

器官特定项目

• 批准号: 10685591
• 负责人: CLAYTON E MATHEWS
• 金额: $ 158.75万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-10 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10685591
• 关键词: 3-Dimensional; Actins; Address; African American; Age; Age Years; Amylases; Anatomy; Antibodies; Antigen-Presenting Cells; Arteries; Atlases; Bioinformatics; Biology; Biomedical Research; Catalogs; Caucasians; Cd68; Cell Nucleus; Cells; Collaborations; Collagen; Collection; Cytometry; Data; Data Analyses; Diabetes Mellitus; Disease; Drug or chemical Tissue Distribution; Duct (organ) structure; Equipment and supply inventories; Ethnic Origin; Female; Fibrosis; Florida; Future; Genetic; Goals; Guidelines; Head of pancreas; Hispanic; Human; Human BioMolecular Atlas Program; Image; Incidence; Information Distribution; Infrastructure; Knowledge; Link; Lipids; Location; Lymphocyte; Malignant neoplasm of pancreas; Maps; Mass Spectrum Analysis; Measurement; Metabolism; Metadata; Molecular; Molecular Profiling; NCAM1 gene; Nerve; Online Systems; Organ; Organ Procurements; Outcome; PECAM1 gene; PTPRC gene; Pancreas; Pancreatic Diseases; Pancreatitis; Peroxidases; Physiology; Process; Production; Proteins; RNA; Race; Regulation; Research; Resolution; Sampling; Sex Bias; Slide; Smooth Muscle; Specimen; Stains; Structure; Synaptophysin; System; Tail; Technology; Texas; Thick; Tissue and Organ Procurement; Tissues; Transplantation; United States National Institutes of Health; Universities; Vascularization; Work; fluorescence imaging; improved; insight; islet; male; multiple omics; nerve supply; neutrophil; preservation; prognostic; racial population; reconstruction; sex; tissue mapping; tissue processing; tumor; tumorigenesis; type I and type II diabetes; whole slide imaging

PROJECT ABSTRACT The overarching goal of the Human BioMolecular Atlas Program (HuBMAP) and specifically of Tissue Mapping Centers is to generate high resolution 3D biomolecular maps for non-diseased human organs. The overall objective of this pancreas organ specific project (OSP) is to identify and collect whole disease-free pancreas from donors across different sexes and ethnicities, to develop a robust system for tissue processing and distribution, and to generate multi-scale, high resolution, 3D multi-omics molecular atlases of functional regions of human pancreas. This OSP integrates unique expertise ranging from pancreas biology, diabetes, organ procurement, tissue processing, deep-tissue fluorescence imaging analysis (University of Florida), advanced multi-omics mass spectrometry-based tissue mapping (PNNL), and sophisticated high-throughput bioinformatics and 3D reconstruction of molecular maps (Texas Advanced Computing Center) of regions of pancreas, such as islets. Specifically, we will: (1) Identify and collect whole disease-free pancreas from donors through collaboration with the Organ Procurement Organizations (OPO) and Research Intermediaries (RI), and develop infrastructure for timely referral and donor collection, as well as a robust, web-based, specimen inventory and request system that accesses a catalogue of representative sample derivative meta-data (i.e. whole slide image or cytometry) of quality and composition; (2) Provide preserved, embedded, and sectioned pancreas tissue using pancreatic processing processes that have carefully mapped the pancreas in 2D; (3) Perform multi-scale and multi-omic imaging or spatially resolved characterization of pancreas and function-guided tissue sections through the characterization pipeline; and (4) Create comprehensive 3-D biomolecular atlas of pancreas that maps protein, metabolite, and lipid expression throughout functional regions of the pancreas through the Data Analysis Core.

项目摘要 人类生物分子图谱计划（HuBMAP），特别是组织图谱的总体目标 中心的目标是为非患病的人体器官生成高分辨率的3D生物分子图谱。整体 本胰腺器官特异性项目（OSP）的目的是识别和收集完整的无病胰腺 来自不同性别和种族的捐赠者，以开发一个强大的组织处理系统， 分布，并生成多尺度，高分辨率，3D多组学分子图谱的功能 人类胰腺的区域。 该OSP整合了独特的专业知识，包括胰腺生物学，糖尿病，器官采购，组织 处理，深层组织荧光成像分析（佛罗里达大学），先进的多组学质量 基于光谱的组织映射（PNNL），以及复杂的高通量生物信息学和3D 胰腺区域（例如胰岛）的分子图谱重建（德克萨斯州高级计算中心）。 具体而言，我们将：（1）通过合作从供体中识别和收集无病胰腺 与器官采购组织（OPO）和研究中介机构（RI）合作， 及时转诊和供体采集的基础设施，以及强大的基于网络的标本库存， 访问代表性样本衍生元数据（即，整个载玻片图像）的目录的请求系统 或细胞术）的质量和组成;（2）提供保存、包埋和切片的胰腺组织 使用已经在2D中仔细映射胰腺的胰腺处理过程;（3）执行多尺度 以及胰腺和功能引导组织切片的多组成像或空间分辨表征 通过表征管道;以及（4）创建胰腺的全面的3-D生物分子图谱， 通过数据绘制了整个胰腺功能区域的蛋白质、代谢物和脂质表达图。 分析核心