Organ Specific Project

器官特定项目

• 批准号: 10259784
• 负责人: CLAYTON E MATHEWS
• 金额: $ 70.21万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-10 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10259784
• 关键词: 3-Dimensional; Address; African American; Age; Age-Years; Amylases; Anatomy; Antibodies; Antigen-Presenting Cells; Arteries; Atlases; Bioinformatics; Biology; Biomedical Research; Catalogs; Caucasians; Cell Nucleus; Cells; Collaborations; Collagen; Collection; Cytometry; Data; Data Analyses; Diabetes Mellitus; Disease; Drug or chemical Tissue Distribution; Duct (organ) structure; Ensure; Equipment and supply inventories; Ethnic Origin; Female; Fibrosis; Florida; Future; Genetic; Goals; Guidelines; Head of pancreas; Hispanics; Human; Human BioMolecular Atlas Program; Image; Incidence; Information Distribution; Infrastructure; Insulin-Dependent Diabetes Mellitus; Knowledge; Link; Lipids; Location; Lymphocyte; Malignant neoplasm of pancreas; Maps; Mass Spectrum Analysis; Measurement; Metabolism; Metadata; Molecular; Molecular Profiling; NCAM1 gene; Nerve; Non-Insulin-Dependent Diabetes Mellitus; Online Systems; Organ; Organ Procurements; Outcome; PECAM1 gene; PTPRC gene; Pancreas; Pancreatic Diseases; Pancreatitis; Peroxidases; Physiology; Process; Production; Proteins; RNA; Race; Regulation; Research; Resolution; Sampling; Sex Bias; Slide; Smooth Muscle Actin Staining Method; Specimen; Stains; Structure; Synaptophysin; System; Tail; Technology; Texas; Thick; Time; Tissues; Transplantation; United States National Institutes of Health; Universities; Vascularization; Work; base; fluorescence imaging; insight; islet; male; multiple omics; nerve supply; neutrophil; preservation; prognostic; protein metabolite; reconstruction; sex; tissue processing; tumor; tumorigenesis; whole slide imaging

PROJECT ABSTRACT The overarching goal of the Human BioMolecular Atlas Program (HuBMAP) and specifically of Tissue Mapping Centers is to generate high resolution 3D biomolecular maps for non-diseased human organs. The overall objective of this pancreas organ specific project (OSP) is to identify and collect whole disease-free pancreas from donors across different sexes and ethnicities, to develop a robust system for tissue processing and distribution, and to generate multi-scale, high resolution, 3D multi-omics molecular atlases of functional regions of human pancreas. This OSP integrates unique expertise ranging from pancreas biology, diabetes, organ procurement, tissue processing, deep-tissue fluorescence imaging analysis (University of Florida), advanced multi-omics mass spectrometry-based tissue mapping (PNNL), and sophisticated high-throughput bioinformatics and 3D reconstruction of molecular maps (Texas Advanced Computing Center) of regions of pancreas, such as islets. Specifically, we will: (1) Identify and collect whole disease-free pancreas from donors through collaboration with the Organ Procurement Organizations (OPO) and Research Intermediaries (RI), and develop infrastructure for timely referral and donor collection, as well as a robust, web-based, specimen inventory and request system that accesses a catalogue of representative sample derivative meta-data (i.e. whole slide image or cytometry) of quality and composition; (2) Provide preserved, embedded, and sectioned pancreas tissue using pancreatic processing processes that have carefully mapped the pancreas in 2D; (3) Perform multi-scale and multi-omic imaging or spatially resolved characterization of pancreas and function-guided tissue sections through the characterization pipeline; and (4) Create comprehensive 3-D biomolecular atlas of pancreas that maps protein, metabolite, and lipid expression throughout functional regions of the pancreas through the Data Analysis Core.

项目摘要