HORNET Center for Autonomic Nerve Recording and Stimulation Systems (CARSS)

HORNET 自主神经记录和刺激系统中心 (CARSS)

• 批准号: 10706622
• 负责人: Maral Mousavi
• 金额: $ 71.98万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-23 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10706622
• 关键词: Acceleration; Acetylcholine; Adopted; Antibodies; Bladder; Calibration; Catecholamines; Chemicals; Colonic Pouches; Detection; Development; Disease; Electrodes; Elements; Encapsulated; Ensure; Enzymes; Equilibrium; Esophagus; Evaluation; Exocrine Glands; Film; Implant; In Vitro; Individual; Iridium; Label; Liquid substance; Longevity; Lung; Measures; Membrane; Methodology; Modality; Modification; Monitor; Movement; Myocardium; Nervous System; Neuromodulator; Neurons; Norepinephrine; Organ; Organ Temperatures; Parasympathetic Nervous System; Pattern; Performance; Periodicals; Periodicity; Peripheral; Platinum; Polyurethanes; Polyvinyls; Relaxation; Risk; Role; Saline; Scanning; Sense Organs; Serum; Shapes; Smooth Muscle; Sphincter; Stomach; Surface; Surgical sutures; System; Technology; Temperature; Temperature Sense; Testing; Thinness; Work; aptamer; autonomic nerve; cell motility; design; implantation; in vivo; iridium oxide; multiplex detection; neuromuscular; neuroregulation; parylene; parylene C; receptor; sensor

Physical and chemical sensing enables detection of functional states of individual end organs for state- dependent neuromodulation. NEST 5 focuses on developing robust sensors for temperature, motility, pH, acetylcholine, and catecholamines, and performing extensive benchtop testing to ensure reliable in vivo performance for end organ sensing. Proper functioning of end-organs is dependent on the local organ temperature. Therefore, accurate, in vivo temperature sensing of end organs can provide valuable information on their functional states. We will develop micropatterned platinum thin film-based resistive temperature sensors encapsulated in Parylene C for in vivo temperature sensing. Motility of certain end organs is associated with their functional states such as esophagus expansion and sphincter contraction. We will develop resistive and capacitive strain sensors encapsulated in Parylene C or polyurethane for motility sensing of target end organs. We will conformally coat commercial bend sensors with Parylene C as encapsulation and suture them in a pre-bent configuration to convert contraction and expansion to bending deformations. In parallel, we will conformally coat a commercial stretchable capacitive sensor with polyurethane as stretchable encapsulation. Acetylcholine functions both as a neuromuscular transmitter and neuromodulator, and is found in the central and peripheral neurons, in target organs of the parasympathetic nervous systems. NEST 5 develops electrochemical acetylcholine sensor using an enzyme-free sensing membrane doped with an organic and stable receptor. We will integrate the sensing membrane in a Parylene-C based platinum microarray. The end-organ electrochemical sensing of pH can be used for detecting functional states of individual end organs for enabling state-dependent neuromodulation. We will develop a platinum microarray on a Parylene substrate and electrodeposit iridium oxide as the active pH sensing material. The end-organ electrochemical sensing of catecholamines, and norepinephrine (NE) in particular, can be used for detecting the efficacy of autonomic neuromodulation on restoring the sympathetic-parasympathetic balance, as well as functional or disease states in organs and the body. We will develop a platinum microarray on a Parylene substrate and detecting catecholamines using the established methodology of fast scan cyclic voltammetry. Extensive thermally accelerated benchtop testing will be performed in saline for all sensing modules to de-risk the technology for long-term end organ sensing. Electrochemical sensing modules will be tested pooled serum media (in addition to saline solutions) for evaluation of sensor biofouling.

物理和化学感测使得能够检测各个终末器官的功能状态， 依赖性神经调节NEST 5专注于开发用于温度，运动，pH， 乙酰胆碱和儿茶酚胺，并进行广泛的实验室测试，以确保可靠的体内 用于末端器官感测的性能。 终末器官的正常功能取决于局部器官的温度。因此，准确，在体内 末端器官的温度感测可以提供关于其功能状态的有价值的信息。我们将开发 微图案化铂薄膜基电阻温度传感器封装在聚对二甲苯C体内 温度传感 某些终末器官的运动与其功能状态相关，例如食管扩张和 括约肌收缩我们将开发封装在聚对二甲苯C或 用于目标终末器官的运动感测的聚氨酯。我们将在商用弯曲传感器上涂覆 聚对二甲苯C作为封装，并将其缝合在预弯曲配置中，以转换收缩和扩张 弯曲变形。与此同时，我们将共形地涂覆商业可拉伸电容传感器， 聚氨酯作为可拉伸封装。 乙酰胆碱作为神经肌肉递质和神经调质发挥作用，并在中枢神经系统中发现。 和外周神经元，在副交感神经系统的靶器官中。NEST 5开发 电化学乙酰胆碱传感器，其使用掺杂有有机且稳定的 受体的我们将传感膜集成在基于Parylene-C的铂微阵列中。终末器官 pH的电化学感测可用于检测各个终末器官的功能状态 状态依赖性神经调节我们将在聚对二甲苯基底上开发铂微阵列， 电沉积氧化铱作为活性pH传感材料。 儿茶酚胺，特别是去甲肾上腺素（NE）的终末器官电化学传感可以是 用于检测自主神经调节对恢复交感-副交感神经的功效 平衡，以及器官和身体的功能或疾病状态。我们将开发一种铂微阵列 在Parylene基板上，并使用快速扫描循环的既定方法检测儿茶酚胺 伏安法将在生理盐水中进行广泛的热加速台架测试，以进行所有感知 模块，以降低长期终末器官传感技术的风险。电化学传感模块将是 测试了合并的血清培养基（除盐水溶液外），用于评价探头生物污染。