HORNET Center for Autonomic Nerve Recording and Stimulation Systems (CARSS)

HORNET 自主神经记录和刺激系统中心 (CARSS)

• 批准号: 10557006
• 负责人: Maral Mousavi
• 金额: $ 76.71万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-23 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10557006
• 关键词: Acetylcholine; Adopted; Antibodies; Bladder; Calibration; Catecholamines; Chemicals; Colonic Pouches; Detection; Development; Disease; Electrodes; Elements; Encapsulated; Ensure; Enzymes; Equilibrium; Esophagus; Evaluation; Exocrine Glands; Film; Implant; In Vitro; Individual; Iridium; Label; Liquid substance; Longevity; Lung; Measures; Membrane; Methodology; Modality; Modification; Monitor; Movement; Myocardium; Nervous system structure; Neuromodulator; Neurons; Norepinephrine; Organ; Organ Temperatures; Parasympathetic Nervous System; Performance; Periodicity; Peripheral; Platinum; Polyurethanes; Polyvinyls; Relaxation; Risk; Role; Saline; Scanning; Sense Organs; Serum; Shapes; Smooth Muscle; Sphincter; Stomach; Stretching; Surface; Surgical sutures; System; Technology; Temperature; Temperature Sense; Testing; Thinness; Work; aptamer; autonomic nerve; base; cell motility; design; implantation; in vivo; iridium oxide; multiplex detection; neuromuscular; neuroregulation; parylene; parylene C; receptor; sensor

Physical and chemical sensing enables detection of functional states of individual end organs for state- dependent neuromodulation. NEST 5 focuses on developing robust sensors for temperature, motility, pH, acetylcholine, and catecholamines, and performing extensive benchtop testing to ensure reliable in vivo performance for end organ sensing. Proper functioning of end-organs is dependent on the local organ temperature. Therefore, accurate, in vivo temperature sensing of end organs can provide valuable information on their functional states. We will develop micropatterned platinum thin film-based resistive temperature sensors encapsulated in Parylene C for in vivo temperature sensing. Motility of certain end organs is associated with their functional states such as esophagus expansion and sphincter contraction. We will develop resistive and capacitive strain sensors encapsulated in Parylene C or polyurethane for motility sensing of target end organs. We will conformally coat commercial bend sensors with Parylene C as encapsulation and suture them in a pre-bent configuration to convert contraction and expansion to bending deformations. In parallel, we will conformally coat a commercial stretchable capacitive sensor with polyurethane as stretchable encapsulation. Acetylcholine functions both as a neuromuscular transmitter and neuromodulator, and is found in the central and peripheral neurons, in target organs of the parasympathetic nervous systems. NEST 5 develops electrochemical acetylcholine sensor using an enzyme-free sensing membrane doped with an organic and stable receptor. We will integrate the sensing membrane in a Parylene-C based platinum microarray. The end-organ electrochemical sensing of pH can be used for detecting functional states of individual end organs for enabling state-dependent neuromodulation. We will develop a platinum microarray on a Parylene substrate and electrodeposit iridium oxide as the active pH sensing material. The end-organ electrochemical sensing of catecholamines, and norepinephrine (NE) in particular, can be used for detecting the efficacy of autonomic neuromodulation on restoring the sympathetic-parasympathetic balance, as well as functional or disease states in organs and the body. We will develop a platinum microarray on a Parylene substrate and detecting catecholamines using the established methodology of fast scan cyclic voltammetry. Extensive thermally accelerated benchtop testing will be performed in saline for all sensing modules to de-risk the technology for long-term end organ sensing. Electrochemical sensing modules will be tested pooled serum media (in addition to saline solutions) for evaluation of sensor biofouling.

物理和化学传感使检测单个末端器官的功能状态成为可能 依赖神经调节。Nest 5专注于开发坚固的温度、运动、pH、 乙酰胆碱和儿茶酚胺，并进行广泛的台式测试，以确保可靠的体内 用于末端器官检测的性能。 末端器官的正常功能取决于局部器官的温度。因此，准确的，活体的 末端器官的温度传感可以提供有关其功能状态的有价值的信息。我们将发展 聚对二甲苯C封装的微图案化铂薄膜电阻温度传感器的体内应用 温度感应。 某些末端器官的运动与它们的功能状态有关，如食道扩张和 括约肌收缩。我们将开发封装在对二甲苯或C中的阻性和电容式应变传感器 用于目标末端器官运动感测的聚亚安酯。我们将在商用弯曲传感器上涂上 并将其缝合在预弯曲配置中，以转换收缩和膨胀 到弯曲变形。同时，我们将对商用可伸缩电容传感器进行共形涂层 可伸缩封装的聚亚安酯。 乙酰胆碱既是一种神经肌肉递质，也是一种神经调节剂，在中枢 和周围神经元，在副交感神经系统的靶器官中。Nest 5开发 电化学乙酰胆碱传感器采用无酶敏感膜掺杂有机且稳定 受体。我们将把传感膜集成到基于对二甲苯-C的铂微阵列中。末日风琴 PH的电化学传感可用于检测单个末端器官的功能状态，以使 状态依赖的神经调节。我们将在对二甲苯衬底上开发铂微阵列，并 电沉积氧化Ir作为活性pH敏感材料。 儿茶酚胺，特别是去甲肾上腺素(NE)的末端器官电化学传感可以是 用于检测自主神经调节恢复交感-副交感神经的效果 平衡，以及器官和身体的功能或疾病状态。我们将开发一种铂金微阵列 快速扫描循环检测儿茶酚胺的研究 伏安法。对于所有感测，将在生理盐水中执行广泛的热加速台式测试 模块，以降低长期末端器官检测技术的风险。电化学传感模块将被 用于评估传感器生物污垢的测试混合血清介质(除生理盐水外)。