Behavior Core

行为核心

• 批准号: 10705994
• 负责人: Adam Christopher Mar
• 金额: $ 57.84万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705994
• 关键词: 3-Dimensional; Affect; Agonistic Behavior; Animal Behavior; Animal Welfare; Animals; BRAIN initiative; Behavior; Behavior assessment; Behavior monitoring; Behavioral; Biological Assay; Breeding; Collaborations; Communication; Complex; Computer Vision Systems; Computer software; Computers; Core Facility; Custom; Darkness; Data; Data Analyses; Data Collection; Data Science Core; Data Set; Decision Making; Detection; Development; Ensure; Environment; Ethology; Experimental Designs; Funding; Genetic; Home; Hour; Housing; Image; Individual; Infrastructure; Investments; Laboratories; Light; Machine Learning; Measures; Methods; Modality; Molecular; Monitor; Mus; Neurosciences; Oxytocin; Parents; Partner in relationship; Postdoctoral Fellow; Posture; Protocols documentation; Quantitative Evaluations; Research; Resources; Rodent; Scientist; Social Development; Social Environment; Social Hierarchy; Social Interaction; Spatial Behavior; Specific qualifier value; Standardization; System; Techniques; Testing; Training; Transgenic Mice; Validation; Vendor; Work; affiliative behavior; analysis pipeline; analytical tool; behavior test; commune; data pipeline; data streams; deep learning; design; experience; facility renovation; flexibility; improved; information processing; innovation; learning network; medical schools; member; neural; neural circuit; neuroregulation; next generation; novel; optogenetics; parallel processing; predictive modeling; social; success; tool; touchscreen; training project

Project Summary: Behavior Core Long-term (days to months) and continuous (24 hours/day, 7 days/week) behavioral monitoring of mouse socio- spatial behavior is fundamental to this BRAIN Initiative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior”. Each Project requires behavioral assessment of mice in home cages or in larger more complex environments, often longitudinally and in conjunction with neural recordings, optogenetics, or other manipulations of cellular and network-level activity. A number of recent technical advances across levels now enable a deeper understanding of neuromodulation on flexible information processing and behavior in mice. Some of these innovations have been pioneered by our laboratories and the Projects/Cores of the Oxytocin U19 in the previous funding cycle. Specifically, it is now possible- if not routine- to apply advanced machine learning and computer vision methods for quantitative behavioral analysis of animal poses and trajectories from video data. This includes video tracking of multiple animals interacting, from different camera angles, across the light/dark cycle, and with simultaneous neural recordings. Over the last five years we have developed hardware, software, and other infrastructure to perform this continuous behavioral monitoring in our facility. Our successes have led to considerable investment in space and computer storage for this facility by NYU Langone. We now capitalize on the development of this facility and behavioral monitoring system to support the next-generation studies of multi-animal behavior and modulation of neural circuit function by the Oxytocin U19 teams. Aim 1 is to improve our capacity for behavioral home cage monitoring, both in terms of number of animals monitored per cage and the pipeline for data collection and analysis. Aim 2 is to develop more complex housing environments for studies of socio-spatial behavior and decision-making in mouse colonies of small to moderate sizes. Aim 3 is to provide a suite of additional social, spatial and other oxytocin-related testing available to Project teams and assisted by Behavior Core staff, including breeding and housing the set of transgenic mouse lines required for this U19 proposal.

项目概要：行为核心 长期（数天至数月）和连续（24小时/天，7天/周）的小鼠社会行为监测， 空间行为是BRAIN Initiative关于“神经回路催产素调节”的提案的基础 功能与行为”。每个项目都要求对饲养在笼子里或更大笼子里的小鼠进行行为评估。 复杂的环境，通常是纵向的，并结合神经记录，光遗传学，或其他 操纵细胞和网络级活动。现在，许多最近的技术进步跨越了各个级别， 使我们能够更深入地了解神经调节对小鼠灵活的信息处理和行为的影响。 其中一些创新是由我们的实验室和催产素U19的项目/核心开创的 在上一个融资周期。具体来说，现在可以-如果不是常规-应用先进的机器学习 和计算机视觉方法，用于从视频中定量分析动物姿势和轨迹 数据这包括视频跟踪多个动物的互动，从不同的相机角度，在整个 光/暗周期，并同时进行神经记录。在过去的五年里，我们开发了硬件， 软件和其他基础设施，以在我们的设施中执行这种持续的行为监测。我们的成功 这使得纽约大学Langone在空间和计算机存储方面进行了大量投资。 我们现在利用这一设施和行为监测系统的发展， 下一代多动物行为研究和催产素U19对神经回路功能的调节 团队目的1是提高我们的能力，行为的家庭笼监测，无论是在数量的动物 监测每个笼子和管道的数据收集和分析。目标二是开发更复杂的住房 环境的社会空间行为和决策的研究在小鼠殖民地的小到中等 尺寸.目标3是为项目提供一套额外的社会、空间和其他催产素相关测试 团队和行为核心工作人员的协助下，包括育种和住房的转基因小鼠品系 这是U19的要求。