Quantitative 3D imaging of in situ nanoparticle movement and cellular behavior during neuroinflammation

神经炎症过程中纳米粒子原位运动和细胞行为的定量 3D 成像

• 批准号: 10708728
• 负责人: Elizabeth A Nance
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10708728
• 关键词: 3-Dimensional; Adult; Behavior; Biological Assay; Biological Sciences; Brain; Brain Diseases; Cells; Central Nervous System Diseases; Clinical Sciences; Developmental Process; Disease; Environment; Excision; Foundations; Inflammation; Injury; Lead; Methodology; Modeling; Molecular; Movement; Neurologic Process; Process Assessment; Rattus; Regulation; Research; Severity of illness; Slice; Technology; Therapeutic; Three-Dimensional Imaging; Time; Transgenic Organisms; Translating; Translations; autism spectrum disorder; cell behavior; cellular imaging; fluid flow; glymphatic system; human disease; imaging modality; in situ imaging; in vivo; insight; nanoparticle; neonatal brain; nervous system disorder; neuroinflammation; perinatal brain; quantitative imaging; synaptogenesis; therapeutic target; wasting

PROJECT SUMMARY Currently, the means to gather real-time molecular information from the diseased human brain is limited, and high-throughput platforms that can assay neurological disease severity representative of the in vivo environment are still lacking. This proposal reflects a foundational effort in my lab to generate a high throughout, quantitative, real-time method of imaging cell and nanoparticle behavior, in 3-dimensions, within the neonatal or perinatal brain in the presence of disease. We will specifically focus on neuroinflammation as a common disease hallmark and use a transgenic rat model of autism. This approach will open up new avenues of research in the life sciences and clinical sciences, by providing a platform for assessment of processes that are currently inaccessible to high-throughput study, including developmental processes (i.e. synaptogenesis) and normal regulation of fluid flow (i.e. regulation of waste removal via the glymphatic system). In addition, tracking and modelling nanoparticle co-localization in, or interaction with, cells following injury could also provide new potential therapeutic targets. Developing therapeutic technologies by leveraging their in vivo interactions with common disease hallmarks can lead to more efficient translation of therapies across diseases with shared pathophysiological features. Given that inflammation is a common factor across many central nervous system (CNS) diseases, results are expected to provide insights and translate from the autism model used in this proposal to other models, including adult, of brain disease.

项目总结

项目成果

Nanotherapeutics and the Brain.

• DOI: 10.1146/annurev-chembioeng-092220-030853
• 发表时间: 2022-06-10
• 期刊: Annual review of chemical and biomolecular engineering
• 影响因子: 8.4
• 作者: Joseph A;Nance E
• 通讯作者: Nance E

Nanotherapeutic modulation of excitotoxicity and oxidative stress in acute brain injury.

• DOI: 10.1177/1849543520970819
• 发表时间: 2020-01
• 期刊: Nanobiomedicine
• 作者: Liao R;Wood TR;Nance E
• 通讯作者: Nance E

Multiple Particle Tracking Detects Changes in Brain Extracellular Matrix and Predicts Neurodevelopmental Age.

• DOI: 10.1021/acsnano.1c00394
• 发表时间: 2021-05-25
• 期刊: ACS nano
• 影响因子: 17.1
• 作者: McKenna M;Shackelford D;Ferreira Pontes H;Ball B;Nance E
• 通讯作者: Nance E

diff_classifier: Parallelization of multi-particle tracking video analyses.

• DOI: 10.21105/joss.00989
• 发表时间: 2019-01-01
• 期刊: Journal of open source software
• 作者: Curtis, Chad;Rokem, Ariel;Nance, Elizabeth
• 通讯作者: Nance, Elizabeth