Computational studies of membrane transport proteins

膜转运蛋白的计算研究

基本信息

项目摘要

Secondary active transporters are a class of membrane proteins that use pre-existing molecular concentration gradients as an energy source for translocating another substrate, such as a nutrient or a neurotransmitter, against its concentration gradient. This process requires the protein to change conformations so as to expose a pathway to the substrate binding site(s) on one or other side of the membrane, in a cycle known as alternating access. Every organism expresses dozens of different secondary transporter proteins, and these exhibit a diverse set of architectures, albeit always with some form of internal structural symmetry. Unprecedented, ground-breaking insights have been garnered from three-dimensional structures obtained in the last decade. Nevertheless, a detailed understanding of the mechanism of each membrane transport protein, and how it is regulated by factors in its environment, requires knowledge of its structure in many more conformational states, including identification of the binding regions for the substrate or substrates. In addition, highly-dynamic segments at either end of the protein, which are often responsible for regulatory roles, require additional effort to characterize. Ongoing studies from our group over the last year have continued to investigate these questions for several different membrane proteins, including serotonin transporter SERT, the betaine symporter BetP, and mitochondrial pyruvate carrier MPC, using advanced structure prediction techniques and molecular dynamics simulations. These efforts are being carried out in collaboration with experimental laboratories, to drive understanding of the mechanism of membrane transport and regulation.
二级活性转运蛋白是一类膜蛋白,它们利用预先存在的分子浓度梯度作为能量来源,使另一种底物(如营养物质或神经递质)逆其浓度梯度进行易位。这个过程需要蛋白质改变构象,以便在膜的一侧或另一侧暴露一条通往底物结合位点的途径,这是一个被称为交替进入的循环。每个生物体都表达几十种不同的二级转运蛋白,这些转运蛋白表现出不同的结构,尽管总是具有某种形式的内部结构对称。在过去的十年里,人们从三维结构中获得了前所未有的、突破性的见解。然而,要详细了解每种膜转运蛋白的机制,以及它如何受到环境因素的调节,需要了解其在许多构象状态下的结构,包括鉴定底物或底物的结合区域。此外,蛋白质两端的高动态片段通常负责调节作用,需要额外的努力来表征。在过去的一年里,我们小组正在进行的研究继续研究几种不同的膜蛋白,包括血清素转运体SERT,甜菜碱同调体BetP和线粒体丙酮酸载体MPC,使用先进的结构预测技术和分子动力学模拟。这些努力正在与实验实验室合作进行,以推动对膜运输和调节机制的理解。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Lucy Forrest其他文献

Lucy Forrest的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Lucy Forrest', 18)}}的其他基金

Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    9563174
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    10708625
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    10018696
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    10915991
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    10263051
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    8940130
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Computational studies of membrane transport proteins
膜转运蛋白的计算研究
  • 批准号:
    9358608
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Computational studies of membrane transport proteins
膜转运蛋白的计算研究
  • 批准号:
    10263049
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Development and assessment of methods for membrane protein structure prediction
膜蛋白结构预测方法的开发和评估
  • 批准号:
    9358610
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:
Computational studies of membrane transport proteins
膜转运蛋白的计算研究
  • 批准号:
    10915989
  • 财政年份:
  • 资助金额:
    $ 116.2万
  • 项目类别:

相似海外基金

Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
  • 批准号:
    MR/S03398X/2
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
  • 批准号:
    EP/Y001486/1
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
  • 批准号:
    2338423
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
  • 批准号:
    MR/X03657X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
  • 批准号:
    2348066
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Standard Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
  • 批准号:
    2341402
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
  • 批准号:
    AH/Z505481/1
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10107647
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    EU-Funded
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10106221
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
  • 批准号:
    AH/Z505341/1
  • 财政年份:
    2024
  • 资助金额:
    $ 116.2万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了