Engineering hydrophilic/amphiphilic Vitamin B6-based super antioxidant dendrimers for controlling chronic inflammation

工程设计亲水性/两亲性维生素 B6 超级抗氧化树枝状聚合物，用于控制慢性炎症

• 批准号: 10798901
• 负责人: Choon Young Lee
• 金额: $ 10万
• 依托单位: CENTRAL MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798901
• 关键词: Acceleration; Administrative Supplement; Anti-Inflammatory Agents; Antioxidants; Architecture; Arthritis; Asthma; Atherosclerosis; Cells; Chronic; Communities; Data; Dendrimers; Diabetes Mellitus; Disease; Electrospray Ionization; Employment; Engineering; Free Radicals; Funding; Future; Generations; Grant; Inflammation; Malignant Neoplasms; Measures; Michigan; Molecular; Molecular Weight; Monitor; Neurodegenerative Disorders; Oxidative Stress; Prevention; Reaction; Reader; Research; Science; Students; Techniques; Testing; Thin Layer Chromatography; Time; Training; Universities; Vitamin B6; Work; amphiphilicity; cost; graduate school; hydrophilicity; instrument; ionization technique; mass spectrometer; protective effect; public health relevance; skills; undergraduate student

Project Summary Funds are requested to purchase a new Advion CMS Expression-L Electrospray Ionization (ESI) Mass Spectrometer (MS) with a Thin Layer Chromatography (TLC) reader, which will be installed in the lab 343A of the Dow Science building at Central Michigan University (CMU). Electrospray Ionization (ESI) Mass Spectrometer (MS) measures molecular weights (MW) of molecules. For dendrons/dendrimers synthesis, molecular weight is the most critical information for molecular identification because other analytical techniques, such as NMR, cannot readily distinguish between the target dendrimer and incomplete reaction intermediates and dendrimers in different generations. Electrospray ionization (ESI) is a soft ionization technique, causing little to no fragmentation, which is an important criterion for analysis of dendrons and dendrimers with high molecular weights. Without an ESI instrument, our current grant- funded research will be greatly impacted because MS data is required at each intermediate step to move towards the target molecule synthesis. Currently there is no reliable, well-functioning ESI MS in the entire CMU community. The TLC plate reader measures molecular weight of each spot on the TLC plate and help locate the target dendrons (dendrimers) rapidly, contributing to enhancing work efficiency and saving overall cost and time in purification. In summary, (i) ESI MS is the most important analytical instrument for monitoring synthesis reactions of dendrimers/dendrons. (ii) This supplementary funding will allow us to train undergraduate/graduate research students. We believe that procurement of a state-of-the-art analytical instrument (ESI MS spectrometer) with this Administrative Supplement will help accelerate the pace of the proposed research as well as help students acquire a cornerstone MS technique, which is a desirable skill for future employment and graduate school.

项目摘要 申请资金用于购买新的Advion CMS Expression-L电喷雾电离（ESI）质谱仪 配备薄层色谱（TLC）读取器的光谱仪（MS），将安装在实验室中 343 A的陶氏科学大楼在中央密歇根大学（CMU）。电喷雾电离（ESI） 质谱仪（MS）测量分子的分子量（MW）。对于树枝状分子/树枝状聚合物 分子量是分子鉴定的最关键信息，因为其他 分析技术，如NMR，不能容易地区分目标树枝状聚合物和 不完全反应中间体和树枝状大分子。电喷雾离子化（ESI） 一种软电离技术，几乎不产生碎片，这是分析的一个重要标准 具有高分子量的树枝状分子和树枝状聚合物。如果没有ESI仪器，我们现在的拨款- 资助的研究将受到很大影响，因为在每个中间步骤都需要MS数据 目标分子的合成。目前，在整个国家还没有可靠的、功能良好的ESI MS。 CMU社区TLC酶标仪测量TLC板上每个斑点的分子量， 快速定位目标树枝状分子，有助于提高工作效率和节约成本 总成本和纯化时间。总之，（i）ESI MS是最重要的分析仪器 用于监测树枝状聚合物/树枝状基元的合成反应。(ii)这笔补充资金将使我们 培养本科/研究生研究生。我们认为，采购最先进的 分析仪器（ESI MS光谱仪）与本行政补充将有助于加快 建议的研究的步伐，以及帮助学生获得一个基石MS技术，这是一个 未来就业和研究生院所需的技能。