Genetic factors controlling the intensity of social behavior

项目摘要 父母奖励（R35GM119844）的此设备补充的目标是扩展 多光子成像显微镜在Asahina实验室，使神经活动在大脑中的一个活苍蝇可以 在实验者施加复杂的感官刺激和药理学试剂的同时进行监测， 光遗传学和热遗传学操纵特定的神经元群体，并记录行为。到 为了实现父母补助金中概述的目标，越来越有必要了解生理学 攻击控制电路的动力学。要计算一个特定社会行为的相对成本和收益， 根据上下文依赖的方式，苍蝇的大脑必须放大或减少相关的感觉信息， 动物的营养和激素状况，以及每个动物独特的个体行为史 单独的.目前在接受者实验室中可用的功能成像装置不足以定量地 检测感官线索的上下文相关表示，因为过时的显微镜设计 限制了物镜透镜附近的可用空间。拟议购买的设备是 带有电动XY载物台的龙门架。框架的简化支撑结构将消除 物镜透镜周围的显微镜不必要的空间填充部分，而载物台提供了 足够的安装空间，用于设置多个刺激输送装置、LED、热探头和其他设备 用于神经元操作。这些特点将极大地促进成功地完成 父母补助金

Project Summary The goal of this equipment supplement for the parental award (R35GM119844) is to expand the capability of multiphoton imaging microscopy in the Asahina lab so that neural activities in the brain of a live fly can be monitored while the experimenter applies complex sensory stimuli and pharmacological reagents, optogenetically and thermogenetically manipulates specific neuronal population, and records behavior. To achieve the goals outlined in the parental grant, it is increasingly necessary to understand physiological dynamics of aggression-controlling circuits. To calculate relative cost and benefit of a given social behavior in a context-dependent manner, the fly brain must amplify or diminish relevant sensory information according to the animal’s nutritional and hormonal conditions, as well as the individual behavioral history unique to each individual. The functional imaging setup currently available in the recipient’s lab is inadequate to quantitatively detect context-dependent representation of sensory cues, because the outdated design of the microscopy constrains the space available near the objective lens. The proposed piece of equipment for purchase is a gantry frame with a motorized XY-stage. The simplified support structure of the frame will eliminate unnecessary space-filling parts of the microscopy that surround the objective lens, while the stage provides a mounting space sufficient for setting up multiple stimulus-delivery setups, LED, heat probe, and other devices for neuronal manipulations. These features will greatly facilitate the successful completion of the goals of the parental grant.