Diversity Supplement for Updating the Mixture in Mechanisms of Phthalate Toxicity in the Ovary

用于更新卵巢邻苯二甲酸盐毒性机制混合物的多样性补充

基本信息

批准号：
10818882
负责人：
Genoa R Warner
金额：
$ 7.42万
依托单位：
NEW JERSEY INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-05 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10818882
关键词：
Acceleration Affinity Applications Grants Behavior Biochemical Biological Biology Cell Culture Techniques Cell membrane Cells Cessation of life Chemical Structure Chemicals Chemistry Child Complex Culture Media DNA-Binding Proteins Data Development Dioxins Disease Endocrine Disruptors Endocrine disruption Environment Environmental Health Environmental Science Enzymes Equipment Esters Exposure to Family Female Female infertility Funding Gene Expression General Population Genes Genetic Transcription Genomics Hormones Human Hydrocarbons Illinois Image In Vitro Industrialization Infertility Label Laboratories Learning Length Mass Spectrum Analysis Massive Parallel Sequencing Mediating Medical Mentors Mentorship Metabolism Methods Modernization Molecular Nature New Jersey Oocytes Ovarian Ovarian Follicle Ovarian Tissue Ovarian aging Ovary Phthalic Acids Plasticizers Population Postbaccalaureate Premature Ovarian Failure Prevalence Prevention Property Proteins Public Health Reproduction Research Research Personnel Research Training Sampling Side Solvents Structure Techniques Technology Testing Tissues Toxic effect Toxicology Toy Training Universities Update Urine Visualization Woman building materials career career development cell type chromatin immunoprecipitation consumer product environmental chemical exposure exposed human population hands on research imaging modality innovation insight lipophilicity men mouse model next generation sequencing novel ovotoxicity parent grant parent project personal care products phthalates post-doctoral training professor receptor reproductive reproductive organ reproductive system disorder screening skills small molecule toxicant transcription factor

项目摘要

PROJECT SUMMARY/ABSTRACT This is a proposal for a Diversity Supplement to fund hands-on research training and career development for a promising research technician receiving post-baccalaureate training at the New Jersey Institute of Technology. The parent grant for this proposal is R00ES031150 to facilitate Dr. Genoa Warner’s transition to an independent research career as an Assistant Professor in the Department of Chemistry and Environmental Science at the New Jersey Institute of Technology, where she draws from her diverse scientific background in chemistry and biology to investigate important research questions in environmental health. Dr. Warner has extensive training in endocrine disruption and sustainable chemistry and has completed postdoctoral training in reproductive toxicology at the University of Illinois under the mentorship of Dr. Jodi A. Flaws, where she has learned technical and profession skills necessary for her to become a successful independent investigator and leader in the field of reproductive toxicology. In her new laboratory, Dr. Warner investigates the mechanisms by which phthalates, a group of endocrine disrupting chemicals with ubiquitous human exposure, cause ovarian toxicity. Further, she investigates the metabolism of phthalates by ovarian tissue because phthalate metabolites mediate phthalate- inducted toxic effects, contributing to infertility and premature ovarian failure. In the prosed studies, Dr. Warner will mentor the Diversity Supplement candidate, Stephanie Martinez, in pursuing research within the scope of the original project aims to craft her own research niche. Specific Aim 1 of the parent project aims to identify how phthalates partition into ovarian tissue during culture via mass spectrometry and imaging methods using chemically modified phthalates. Specific Aim 2 will determine how phthalate toxicity varies between cell types of the ovary using next generation sequencing techniques. Finally, Specific Aim 3 will identify the genomic localization of phthalates in the ovary by adapting the biochemical technique of chemical affinity capture paired with massively parallel DNA sequencing (Chem-seq) to provide information on the genes and proteins targeted by phthalates. Overall, the proposed studies will provide novel chemical and mechanistic insight into causes of phthalate-induced toxicity in the ovary that contribute to female infertility and premature ovarian failure. The methods combine chemical and biological techniques to develop a new, widely applicable technology for investigating the mechanisms of endocrine disrupting chemicals.

项目摘要/摘要这是对多样性补充的一项建议，以资助动手研究培训和职业发展有前途的研究技术接受新泽西理工学院的学院后培训。该提案的父母赠款为R00ES031150，以促进Genoa Warner博士向独立研究职业是化学与环境科学系的助理教授新泽西理工学院，她从农业潜水员的化学科学背景和生物学研究环境健康中重要的研究问题。华纳博士接受了广泛的培训在内分泌中断和可持续化学中，已经完成了生殖的博士后培训在Jodi A. Flaws博士的心态下，伊利诺伊大学的毒理学学会了技术她成为成功的独立调查员和领导者所必需的职业技能生殖毒理学。华纳博士在她的新实验室中调查了邻苯二甲酸盐的机制一组无处不在的人类暴露的内分泌破坏化学物质，引起卵巢毒性。此外，她研究卵巢组织邻苯二甲酸盐的代谢，因为邻苯二甲酸酯代谢物介导邻苯二甲酸酯 - 诱导的有毒作用，导致不育症和卵巢过早衰竭。在经过证明的研究中，华纳博士将指导多样性补充候选人斯蒂芬妮·马丁内斯（Stephanie Martinez）最初的项目旨在制作自己的研究利基市场。母公司项目的特定目的1旨在确定如何通过质谱和成像方法，使用邻苯二甲酸酯分配到卵巢组织中化学修饰的邻苯二甲酸盐。特定的目标2将决定邻苯二甲酸盐的毒性如何在细胞类型之间变化使用下一代测序技术的卵巢。最后，特定目标3将识别基因组通过调整化学亲和力捕获的生化技术配对的生化技术，将邻苯二甲酸盐定位具有大量平行的DNA测序（Chem-Seq），以提供有关靶向基因和蛋白质的信息通过邻苯二甲酸盐。总体而言，拟议的研究将提供新颖的化学和机械洞察力，以了解邻苯二甲酸酯引起的卵巢毒性导致女性不育症和早产卵巢衰竭。方法结合了化学和生物技术，以开发一种新的，广泛适用的技术研究内分泌破坏化学物质的机制。