Mechanisms of Phthalate Toxicity in the Ovary
邻苯二甲酸盐对卵巢的毒性机制
基本信息
- 批准号:10577981
- 负责人:
- 金额:$ 24.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-05 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:AffinityAwardBehaviorBiochemicalBiologicalBiologyCell Culture TechniquesCell membraneCellsCessation of lifeChemical AgentsChemical StructureChemicalsChemistryChildComplexCulture MediaDNA-Binding ProteinsDataDevelopmentDevelopment PlansDioxinsDiseaseEndocrine DisruptorsEndocrine disruptionEnvironmental HealthEnzymesEquipmentEstersExposure toFamilyFemaleFemale infertilityGene ExpressionGene ProteinsGeneral PopulationGenesGenetic TranscriptionGenomicsGoalsHormonesHumanHydrocarbonsIllinoisImageIn VitroIndividualIndustrializationInfertilityLabelLengthMass Spectrum AnalysisMassive Parallel SequencingMediatingMedicalMentorsMentorshipMetabolismMethodsModernizationMolecularNatureOocytesOvarianOvarian FollicleOvarian TissueOvarian agingOvaryPhasePhthalic AcidsPlasticizersPopulationPostdoctoral FellowPremature Ovarian FailurePrevalencePreventionPropertyPublic HealthReproductionReproductive BiologyResearchResearch PersonnelResearch ProposalsResearch TrainingSamplingSideSolventsStructureTechniquesTechnologyTestingTissuesToxic effectToxicologyToyTrainingUniversitiesUrineVisualizationWomanbuilding materialscareercareer developmentcell typechromatin immunoprecipitationconsumer productenvironmental chemical exposureexposed human populationimaging modalityinnovationinsightlipophilicitymenmouse modelnovelovotoxicitypersonal care productsphthalatesprogramsreceptorreproductivereproductive organreproductive system disorderscreeningskillssmall moleculetoxicanttranscription factor
项目摘要
PROJECT SUMMARY/ABSTRACT
This K99/R00 award will prepare Dr. Genoa Warner for an independent research career, with the main goal of
providing Dr. Warner with all the technical and profession skills necessary for her to become a successful
independent investigator and leader in the field of reproductive toxicology. Dr. Warner has extensive training in
endocrine disruption and sustainable chemistry and is currently a postdoctoral fellow in reproductive toxicology
at the University of Illinois under the mentorship of Dr. Jodi A. Flaws. This award will enable Dr. Warner to expand
her research training in reproductive biology and allow her to draw from her diverse scientific background in
chemistry and biology to investigate important research questions in environmental health. These goals will be
accomplished with the support of Dr. Warner's exceptional mentoring team, composed of Dr. Flaws, Dr. Susan
Schantz, Dr. Heather Stapleton, and Dr. John Katzenellenbogen. The research proposal and career
development plan created by Dr. Warner with the guidance of her mentoring team will provide Dr. Warner with
new experimental techniques and skills needed to establish an independent research program. In the research
training component of this proposal, Dr. Warner will investigate the mechanisms by which phthalates, a group of
endocrine disrupting chemicals with ubiquitous human exposure, cause ovarian follicle toxicity. Further, she will
investigate the metabolism of phthalates by ovarian tissue because phthalate metabolites mediate phthalate-
inducted toxic effects, contributing to infertility and premature ovarian failure. In the prosed studies, Dr. Warner
will use environmentally relevant concentrations of six phthalates as a mixture and individually. Specific Aim 1
(mentored phase) will identify how phthalates partition into ovarian tissue during culture via mass spectrometry
and imaging methods using chemically modified phthalates. Specific Aim 2 (mentored and independent phases)
will determine how phthalate toxicity varies between cell types of the ovary by culturing populations of each cell
type found in the ovarian follicle with phthalates. Finally, Specific Aim 3 (Independent Phase) will identify the
genomic localization of phthalates in the ovary by adapting the biochemical technique of chemical affinity capture
paired with massively parallel DNA sequencing (Chem-seq) to provide information on the genes and proteins
targeted by phthalates. Overall, the proposed studies will provide novel chemical and mechanistic insight into
causes of phthalate-induced toxicity in the ovary that contribute to female infertility and premature ovarian failure.
The methods combine chemical and biological techniques to develop a new, widely applicable technology for
investigating the mechanisms of endocrine disrupting chemicals that will provide the basis for Dr. Warner's
independent career.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Genoa R Warner其他文献
Genoa R Warner的其他文献
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{{ truncateString('Genoa R Warner', 18)}}的其他基金
Diversity Supplement for Updating the Mixture in Mechanisms of Phthalate Toxicity in the Ovary
用于更新卵巢邻苯二甲酸盐毒性机制混合物的多样性补充
- 批准号:
10818882 - 财政年份:2022
- 资助金额:
$ 24.9万 - 项目类别:
Diversity Supplement for Mechanisms of Phthalate Toxicity in the Ovary
卵巢邻苯二甲酸盐毒性机制的多样性补充
- 批准号:
10628940 - 财政年份:2022
- 资助金额:
$ 24.9万 - 项目类别:
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