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Diversity Supplement for Mechanisms of Phthalate Toxicity in the Ovary

卵巢邻苯二甲酸盐毒性机制的多样性补充

基本信息

批准号：
10628940
负责人：
Genoa R Warner
金额：
$ 5.57万
依托单位：
NEW JERSEY INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10628940
关键词：
Affinity Applications Grants Behavior Biochemical Biological Biology Cell Culture Techniques Cell membrane Cells Cessation of life Chemical Agents Chemical Structure Chemicals Chemistry Child Complex Culture Media DNA-Binding Proteins Data Development Dioxins Disease Doctor of Philosophy Endocrine Disruptors Endocrine disruption Environmental Health Environmental Science Enzymes Equipment Esters Exposure to Family Female Female infertility Funding Gene Expression Gene Proteins General Population Genes Genetic Transcription Genomics Hormones Human Hydrocarbons Illinois Image In Vitro Industrialization Infertility Institutes Label Laboratories Length Mass Spectrum Analysis Massive Parallel Sequencing Mediating Medical Mentors Mentorship Metabolism Methods Modernization Molecular Nature New Jersey Oocytes Ovarian Ovarian Follicle Ovarian Tissue Ovarian aging Ovary Phthalic Acids Plasticizers Population Premature Ovarian Failure Prevalence Prevention Property Public Health Reproduction Research Research Personnel Research Training Sampling Side Solvents Structure Talents Techniques Technology Testing Tissues Toxic effect Toxicology Toy Training Universities Urine Visualization Woman building materials career career development cell type chromatin immunoprecipitation consumer product doctoral student environmental chemical exposure exposed human population hands on research imaging modality innovation insight lipophilicity men mouse model next generation sequencing novel ovotoxicity parent grant parent project personal care products phthalates post-doctoral training professor programs receptor reproductive reproductive organ reproductive system disorder screening skills small molecule toxicant transcription factor

项目摘要

PROJECT SUMMARY/ABSTRACT This is a proposal for a Diversity Supplement to fund hands-on research training and career development for a talented doctoral student in the Environmental Science PhD program at the New Jersey Institute of Technology. The parent grant for this proposal is R00ES031150-30 to facilitate Dr. Genoa Warner’s transition to an independent research career as an Assistant Professor in the Department of Chemistry and Environmental Science at the New Jersey Institute of Technology, where she draws from her diverse scientific background in chemistry and biology to investigate important research questions in environmental health. Dr. Warner has extensive training in endocrine disruption and sustainable chemistry and has completed postdoctoral training in reproductive toxicology at the University of Illinois under the mentorship of Dr. Jodi A. Flaws, where she has learned technical and profession skills necessary for her to become a successful independent investigator and leader in the field of reproductive toxicology. In her new laboratory, Dr. Warner investigates the mechanisms by which phthalates, a group of endocrine disrupting chemicals with ubiquitous human exposure, cause ovarian toxicity. Further, she investigates the metabolism of phthalates by ovarian tissue because phthalate metabolites mediate phthalate-inducted toxic effects, contributing to infertility and premature ovarian failure. In the prosed studies, Dr. Warner will mentor the Diversity Supplement candidate, Courtney Potts, in pursuing research within the scope of the original project aims to craft his own research niche. Specific Aim 1 of the parent project aims to identify how phthalates partition into ovarian tissue during culture via mass spectrometry and imaging methods using chemically modified phthalates. Specific Aim 2 will determine how phthalate toxicity varies between cell types of the ovary using next generation sequencing techniques. Finally, Specific Aim 3 will identify the genomic localization of phthalates in the ovary by adapting the biochemical technique of chemical affinity capture paired with massively parallel DNA sequencing (Chem-seq) to provide information on the genes and proteins targeted by phthalates. Overall, the proposed studies will provide novel chemical and mechanistic insight into causes of phthalate-induced toxicity in the ovary that contribute to female infertility and premature ovarian failure. The methods combine chemical and biological techniques to develop a new, widely applicable technology for investigating the mechanisms of endocrine disrupting chemicals.

项目总结/摘要这是一项多样性补充提案，旨在资助实践研究培训和职业发展，她是新泽西理工学院环境科学博士项目的天才博士生。本提案的母公司补助金为R 00 ES 031150 -30，以促进Genoa Warner博士过渡到作为化学与环境系助理教授的独立研究生涯科学在新泽西理工学院，在那里她利用她的多元化的科学背景，化学和生物学研究环境健康的重要研究问题。华纳博士在内分泌干扰和可持续化学方面接受了广泛的培训，并在以下领域完成了博士后培训：生殖毒理学在伊利诺伊大学的指导下，博士Jodi A。她的缺点学习了成为一名成功的独立调查员所需的技术和专业技能，生殖毒理学领域的领导者在她的新实验室里，华纳博士通过以下方式研究了这种机制：邻苯二甲酸酯是一种普遍存在于人体中的内分泌干扰化学物质，毒性此外，她研究了邻苯二甲酸酯通过卵巢组织的代谢，介导邻苯二甲酸酯诱导的毒性作用，导致不孕症和卵巢早衰。在散文中研究，华纳博士将指导多样性补充候选人，考特尼波茨，在追求内部研究最初的项目范围旨在打造自己的研究利基。母项目目标的具体目标1 通过质谱和成像方法确定邻苯二甲酸酯在培养过程中如何分配到卵巢组织中使用化学改性的邻苯二甲酸盐。具体目标2将确定邻苯二甲酸酯毒性如何在细胞间变化卵巢的类型使用下一代测序技术。最后，具体目标3将确定基因组应用化学亲和捕获配对的生物化学技术定位卵巢中的邻苯二甲酸酯大规模并行DNA测序（Chem-seq），以提供靶基因和蛋白质的信息邻苯二甲酸盐总的来说，拟议的研究将提供新的化学和机制的见解的原因，邻苯二甲酸酯诱导的卵巢毒性，导致女性不孕和卵巢早衰。的方法联合收割机结合化学和生物技术，开发一种新的、广泛适用的技术，研究内分泌干扰物的作用机制。