High-Throughput Dried Blood Spot (HT-DBS) Technologies in SARS COV-2 Serology and Vaccinology

SARS COV-2 血清学和疫苗学中的高通量干血斑 (HT-DBS) 技术

基本信息

  • 批准号:
    10855042
  • 负责人:
  • 金额:
    $ 82.46万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-09-30 至 2024-11-30
  • 项目状态:
    已结题

项目摘要

Project Summary As the COVID-19 pandemic continues to spread across the United States it is imperative that we implement technologies to screen large swaths of the population for the presence of antibodies to SARS-CoV-2. Serological surveillance not only affords a measure of virus exposure within a community at large but also provides information necessary to predict outbreak dynamics. Furthermore, as our understanding of how humoral factors contribute to controlling (and possibly exacerbating) COVID-19, it will be essential to have methods in place to measure the “quantity” and “quality” of antibodies associated with both natural SARS-CoV- 2 exposure and candidate SARS-CoV-2 vaccines. This U01 proposal seeks to advance the use of dried blood spots (DBS) in conjunction with a Luminex-based microsphere immunoassay (MIA) to enable high-throughput (HT) population-wide serological surveillance for SARS-CoV-2. Specifically, the proposal will expand the HT- DBS assay to capture the breadth and complexity of SARS-CoV-2 antibody responses following natural infection, and develop a high-throughput competitive immunoassay (CIA) as a surrogate measure of SARS- CoV-2 neutralizing antibody titers in DBS. The proposed platform technologies to be developed at the Wadsworth Center will contribute directly to NCI’s mission to “… develop, validate, improve and implement serological testing and associated technologies…” to address the COVID-19 pandemic.
项目摘要 随着新冠肺炎大流行继续在美国蔓延,我们必须实施 对大范围人群进行SARS-CoV-2抗体筛查的技术。 血清学监测不仅提供了一种衡量整个社区内病毒暴露的方法,而且还 提供预测疫情动态所需的信息。此外,由于我们对如何 体液因素有助于控制(并可能加剧)新冠肺炎,因此有必要 方法测量与天然SARS-CoV-CoV相关的抗体的“量”和“质”。 2暴露和SARS-CoV-2候选疫苗。这项U01提案旨在促进干血的使用 斑点(DBS)与基于Luminex的微球免疫分析(MIA)相结合,实现高通量 (H)SARS-CoV-2全人群血清学监测。具体地说,该提案将扩大HT- DBS试验捕捉SARS-CoV-2抗体应答的广度和复杂性 感染,并开发高通量竞争免疫分析(CIA)作为SARS的替代措施- DBS中的CoV-2中和抗体效价。建议的平台技术在 沃兹沃斯中心将直接为美国国家通信公司的“…”使命做出贡献开发、验证、改进和实施 血清学检测及相关技术…“以应对新冠肺炎大流行。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Slaying SARS-CoV-2 One (Single-domain) Antibody at a Time.
一次杀死SARS-COV-2一种(单域)抗体。
  • DOI:
    10.1016/j.tim.2020.12.006
  • 发表时间:
    2021-03
  • 期刊:
  • 影响因子:
    15.9
  • 作者:
    Czajka TF;Vance DJ;Mantis NJ
  • 通讯作者:
    Mantis NJ
Serological analysis reveals an imbalanced IgG subclass composition associated with COVID-19 disease severity.
  • DOI:
    10.1016/j.xcrm.2021.100329
  • 发表时间:
    2021-07-20
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yates JL;Ehrbar DJ;Hunt DT;Girardin RC;Dupuis AP 2nd;Payne AF;Sowizral M;Varney S;Kulas KE;Demarest VL;Howard KM;Carson K;Hales M;Ejemel M;Li Q;Wang Y;Peredo-Wende R;Ramani A;Singh G;Strle K;Mantis NJ;McDonough KA;Lee WT
  • 通讯作者:
    Lee WT
Use of Self-Collected Dried Blood Spots and a Multiplex Microsphere Immunoassay to Measure IgG Antibody Response to COVID-19 Vaccines.
  • DOI:
    10.1128/spectrum.01336-22
  • 发表时间:
    2023-02-14
  • 期刊:
  • 影响因子:
    3.7
  • 作者:
    Nemeth, Katherine L.;Yauney, Erica;Rock, Jean M.;Bievenue, Rachel;Parker, Monica M.;Styer, Linda M.
  • 通讯作者:
    Styer, Linda M.
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Nicholas J. Mantis其他文献

Collaboration of epithelial cells with organized mucosal lymphoid tissues
上皮细胞与有组织的黏膜淋巴样组织的协作
  • DOI:
    10.1038/ni1101-1004
  • 发表时间:
    2001-11-01
  • 期刊:
  • 影响因子:
    27.600
  • 作者:
    Marian R. Neutra;Nicholas J. Mantis;Jean-Pierre Kraehenbuhl
  • 通讯作者:
    Jean-Pierre Kraehenbuhl
Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in contrived dried blood spots and oral fluids
用于在人为干血斑和口腔液中检测 SARS-CoV-2 抗体的微球免疫测定的实验室间协调
  • DOI:
    10.1128/spectrum.02690-24
  • 发表时间:
    2025-03-31
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Kate L. DeRosa;Nora Pisanic;Kate Kruczynski;Christopher D. Heaney;Linda M. Styer;Nicholas J. Mantis
  • 通讯作者:
    Nicholas J. Mantis
A type-specific B-cell epitope at the apex of outer surface protein C (OspC) of the Lyme disease spirochete, emBorreliella burgdorferi/em
莱姆病螺旋体伯氏疏螺旋体外表面蛋白 C(OspC)顶点的一种特定类型 B 细胞表位
  • DOI:
    10.1128/spectrum.02883-24
  • 发表时间:
    2025-02-14
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    David J. Vance;Grace Freeman-Gallant;Kathleen McCarthy;Carol Lyn Piazza;Yang Chen;Clint Vorauer;Beatrice Muriuki;Michael J. Rudolph;Lisa Cavacini;Miklos Guttman;Nicholas J. Mantis
  • 通讯作者:
    Nicholas J. Mantis
Antibody signatures elicited by potent and subpotent whole-cell pertussis vaccines in mice
强效和次强效全细胞百日咳疫苗在小鼠中引发的抗体特征
  • DOI:
    10.1128/spectrum.03253-24
  • 发表时间:
    2025-03-31
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Yetunde Adewunmi;Jennifer Doering;Prashant Kumar;Jozelyn V. Pablo;Andy A. Teng;Vu Huynh;Kathryn Secrist;David B. Volkin;Sangeeta B. Joshi;Joseph J. Campo;Nicholas J. Mantis
  • 通讯作者:
    Nicholas J. Mantis

Nicholas J. Mantis的其他文献

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{{ truncateString('Nicholas J. Mantis', 18)}}的其他基金

Leveraging a transcription regulatory network to understand Salmonella invasion of host epithelial cells
利用转录调控网络了解沙门氏菌对宿主上皮细胞的侵袭
  • 批准号:
    10154895
  • 财政年份:
    2021
  • 资助金额:
    $ 82.46万
  • 项目类别:
Leveraging a transcription regulatory network to understand Salmonella invasion of host epithelial cells
利用转录调控网络了解沙门氏菌对宿主上皮细胞的侵袭
  • 批准号:
    10374120
  • 财政年份:
    2021
  • 资助金额:
    $ 82.46万
  • 项目类别:
Lyme Disease: B cell epitope discovery and mechanisms of antibody protection
莱姆病:B 细胞表位发现和抗体保护机制
  • 批准号:
    10677521
  • 财政年份:
    2020
  • 资助金额:
    $ 82.46万
  • 项目类别:
Lyme Disease: B cell epitope discovery and mechanisms of antibody protection
莱姆病:B 细胞表位发现和抗体保护机制
  • 批准号:
    10246232
  • 财政年份:
    2020
  • 资助金额:
    $ 82.46万
  • 项目类别:
High-Throughput Dried Blood Spot (HT-DBS) Technologies in SARS COV-2 Serology and Vaccinology
SARS COV-2 血清学和疫苗学中的高通量干血斑 (HT-DBS) 技术
  • 批准号:
    10222023
  • 财政年份:
    2020
  • 资助金额:
    $ 82.46万
  • 项目类别:
Tickborne Disease: B cell epitope discovery and mechanisms of antibody Protection
蜱传疾病:B 细胞表位发现和抗体保护机制
  • 批准号:
    10678249
  • 财政年份:
    2020
  • 资助金额:
    $ 82.46万
  • 项目类别:
High-Throughput Dried Blood Spot (HT-DBS) Technologies in SARS COV-2 Serology and Vaccinology
SARS COV-2 血清学和疫苗学中的高通量干血斑 (HT-DBS) 技术
  • 批准号:
    10688352
  • 财政年份:
    2020
  • 资助金额:
    $ 82.46万
  • 项目类别:
Lyme Disease: B cell epitope discovery and mechanisms of antibody protection
莱姆病:B 细胞表位发现和抗体保护机制
  • 批准号:
    10021076
  • 财政年份:
    2019
  • 资助金额:
    $ 82.46万
  • 项目类别:
Lyme Disease: B cell epitope discovery and mechanisms of antibody protection
莱姆病:B 细胞表位发现和抗体保护机制
  • 批准号:
    10912412
  • 财政年份:
    2019
  • 资助金额:
    $ 82.46万
  • 项目类别:
Mechanisms of IgA - mediated immunity to Vibrio cholerae
IgA 介导的霍乱弧菌免疫机制
  • 批准号:
    9438996
  • 财政年份:
    2017
  • 资助金额:
    $ 82.46万
  • 项目类别:

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