DISPERSION IN MICELLER ELECTROKINETIC CHROMATOGRAPHY

胶束电动色谱中的分散

• 批准号: 2024443
• 负责人: JOE M DAVIS
• 金额: $ 10.14万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2024443
• 关键词: acidity /alkalinity; buffers; chemical kinetics; chromatography; diffusion; electric field; electrochemistry; method development; micelles; temperature

DESCRIPTION: The objective of this study is to determine quantitative theory that explains the dependence of plate number N in micellar electrokinetic chromatography (MEKC) on the parameters, 1) organized-media concentration, 2) organic-modifier concentration, 3) buffer type, 4) buffer concentration, and 5) buffer pH. MEKC is a high-resolution electrokinetic chromatography first reported in 1984 that has been used widely in the analysis of compounds of interest to health-related professions, including porphyrins, haematoporphyrins, derivatized amino acids, motilins, insulins, angiotensins, peptides, xanthines, purins, purines, nucleosides, nucleotides, oligonucleotides, benzo(a)pyrene-adducted DNAs, catechols, catecholamines, vitamins, barbiturates, antibiotics, steroids, and drugs of abuse. The current optimization of MEKC separations is restricted to empirical approaches, however, because theories for N are incomplete. This study will extend and verify theories for N, by which optimization can be achieved. A family of optimizations with respect to different constraints, e.g., resolution, time, cost, etc., can be envisioned. The research method is based on an analytical model for N that has been verified at low electric field strengths. This theory accounts for contributions to N from longitudinal diffusion and plug size. The theory is fairly sophisticated in its ability to account for variations of mobility and diffusion coefficient with temperature. As the above five parameters are varied, deviations between the model s prediction of N and experimentally determined N s can be related quantitatively to measures of dispersion caused by sources other than longitudinal diffusion and plug size. These measures then can be compared to theoretical estimates of dispersion, as calculated by standard means, to conform or refute the validity of the postulated dispersion mechanism. By implementing a systematic experimental program in conjunction with this model, one should make clear the dependence of N on these parameters.

描述：本研究的目的是确定定量

项目成果

Quantitative study of concentration overload, peak asymmetry, and efficiency loss in micellar electrokinetic chromatography.

胶束电动色谱中浓度超载、峰不对称和效率损失的定量研究。

• DOI: 10.1021/ac020376q
• 发表时间: 2002
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Smith,KeithW;Davis,JoeM
• 通讯作者: Davis,JoeM

Dispersion in micellar electrokinetic chromatography.

胶束电动色谱中的分散。

• 发表时间: 2000
• 期刊: Advances in chromatography
• 作者: Davis,JM

Influence of organic modifier concentration on plate number in micellar electrokinetic chromatography. 1. 2-propanol.

有机改性剂浓度对胶束电动色谱中塔板数的影响。

• DOI: 10.1021/ac980417b
• 发表时间: 1998
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Seals,TH;Davis,JM;Murphy,MR;Smith,KW;Stevens,WC
• 通讯作者: Stevens,WC