BRAIN GLUCOSE METABOLISM BY 13C NMR
通过 13C NMR 测定脑葡萄糖代谢
基本信息
- 批准号:2000837
- 负责人:
- 金额:$ 9.49万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-04-01 至 1999-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
APPLICANT'S ABSTRACT: The effects of ethanol on the glutamate and gamma
amino butyric acid (GABA) neurotransmitter systems, and behavioral responses
involving these systems, are well established. Less is known of the
influence of ethanol on the rates of synthesis of the amino acid
neurotransmitters. The overall Objective of this project is to develop
techniques that will allow us to measure the effects of ethanol
administration on the rates of synthesis of the neurotransmitters,
glutamate, GABA and aspartate, from glucose in the intact, conscious rat.
The rates of neurotransmitter synthesis and patterns of glucose metabolism
will be determined using "C NMR spectroscopy to analyze for the distribution
of isotope into glucose metabolites. This technique allows for a
quantitative evaluation of glucose incorporation into metabolites, including
the neurotransmitters, by identifying the distribution of "C into particular
carbon atoms within a metabolite. A long range goal is to determine the
impact of ethanol on the metabolism of glucose through the glycolytic,
anaplerotic and oxidative pathways in order to relate alterations in glucose
metabolism to changes in the rates of neurotransmitter synthesis. Studies
will be carried out utilizing a dose of ethanol which stimulates motor
activity (0.25 g/kg body weight) and one which has a -sedative effect on the
organism (1.0 g/kg body weight). It is expected that these two doses will
influence the patterns of glucose metabolism and neurotransmitter synthesis
differently since it has been previously demonstrated that they exert
opposite effects on the uptake of glucose in certain brain regions.
Techniques will also be developed to study the effects of ethanol on glucose
and neurotransmitter metabolism in mixtures of isolated astrocytes and
neurons (co-cultures). Once this latter technology is established, it will
be utilized in future studies to differentiate between direct and indirect
effects of ethanol on glucose and neurotransmitter metabolism in the brain,
particularly in the cerebral cortex. 1:3C NMR spectroscopy will also be
utilized in development of the techniques for following glucose and
neurotransmitter metabolism in the co-cultures.
申请人摘要:乙醇对谷氨酸和γ
氨基丁酸(GABA)神经递质系统和行为反应
这些系统都是很完善的。 关于这一点,我们所知甚少。
乙醇对氨基酸合成速率的影响
神经传递素 该项目的总体目标是开发
这些技术可以让我们测量乙醇的影响,
给药对神经递质合成速率的影响,
谷氨酸,GABA和天冬氨酸,从葡萄糖在完整的,清醒的大鼠。
神经递质合成速率和糖代谢模式
将使用13 C NMR光谱法来分析分布
同位素转化为葡萄糖代谢物。 这种技术允许一个
葡萄糖掺入代谢物的定量评价,包括
神经递质,通过确定“C”的分布,
代谢物中的碳原子。 长期目标是确定
乙醇通过糖酵解对葡萄糖代谢的影响,
回补和氧化途径,以便与葡萄糖
神经递质合成速率的变化。 研究
将使用一剂乙醇刺激运动,
活性(0.25 g/kg体重)和一个具有镇静作用的
微生物(1.0 g/kg体重)。 预计这两种剂量将
影响葡萄糖代谢和神经递质合成的模式
不同的是,因为以前已经证明,他们发挥
对某些大脑区域葡萄糖摄取的相反影响。
还将开发技术来研究乙醇对葡萄糖的影响
和神经递质代谢的混合物中分离的星形胶质细胞和
神经元(共培养物)。 一旦后一种技术建立起来,它将
在未来的研究中使用,以区分直接和间接
乙醇对大脑中葡萄糖和神经递质代谢的影响,
尤其是大脑皮层 1:3C NMR光谱也将是
用于开发跟踪葡萄糖的技术,
共培养物中的神经递质代谢。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CAROL C CUNNINGHAM其他文献
CAROL C CUNNINGHAM的其他文献
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{{ truncateString('CAROL C CUNNINGHAM', 18)}}的其他基金
Ethanol effects on liver in self-administering primates
乙醇对自我给药灵长类动物肝脏的影响
- 批准号:
6460463 - 财政年份:2002
- 资助金额:
$ 9.49万 - 项目类别:
Ethanol effects on liver in self-administering primates
乙醇对自我给药灵长类动物肝脏的影响
- 批准号:
6731958 - 财政年份:2002
- 资助金额:
$ 9.49万 - 项目类别:
Ethanol effects on liver in self-administering primates
乙醇对自我给药灵长类动物肝脏的影响
- 批准号:
6623039 - 财政年份:2002
- 资助金额:
$ 9.49万 - 项目类别:
ETHANOL CONSUMPTION AND LIVER AND BRAIN METABOLISM
乙醇消耗与肝脏和大脑的代谢
- 批准号:
6629530 - 财政年份:1999
- 资助金额:
$ 9.49万 - 项目类别:
ETHANOL CONSUMPTION AND LIVER AND BRAIN METABOLISM
乙醇消耗与肝脏和大脑的代谢
- 批准号:
6168161 - 财政年份:1999
- 资助金额:
$ 9.49万 - 项目类别:
ETHANOL CONSUMPTION AND LIVER AND BRAIN METABOLISM
乙醇消耗与肝脏和大脑的代谢
- 批准号:
6371234 - 财政年份:1999
- 资助金额:
$ 9.49万 - 项目类别:
ETHANOL CONSUMPTION AND LIVER AND BRAIN METABOLISM
乙醇消耗与肝脏和大脑的代谢
- 批准号:
6509093 - 财政年份:1999
- 资助金额:
$ 9.49万 - 项目类别:
ETHANOL CONSUMPTION AND LIVER AND BRAIN METABOLISM
乙醇消耗与肝脏和大脑的代谢
- 批准号:
2834615 - 财政年份:1999
- 资助金额:
$ 9.49万 - 项目类别:
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