THERMOSTABLE ENZYMES VIA THERMUS GENOME SEQUENCING

通过Thermus基因组测序研究耐热酶

• 批准号: 2423708
• 负责人: VERONIKA VONSTEIN
• 金额: $ 10万
• 依托单位: THERMOGEN, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2423708
• 关键词: bacterial genetics; bacterial proteins; enzymes; microorganism culture; nucleic acid sequence; open reading frames; thermophilic organism; thermostability

DESCRIPTION: (Adapted from the Applicant's Abstract). The investigators propose to determine the complete genome sequence of the extreme thermophile Thermus flavus and assign gene functions to the sequence in order to identify commercially important activities. ThermoGen's chromosomal integration system will be used to verify functional predictions and study phenotypes of ORFs lacking homology with known genes. In Phase I they will optimize sequencing techniques to generate inexpensive and rapid sequence data for this high-GC genome. They expect to sequence 150- 200 kb of the Thermus genome and analyze it by the `Magpie' automated genome investigating environment to find ORFs with potentially important functions. The functions of these ORFs will be verified experimentally. In Phase II they will complete the sequence of the organism and assign functions for most of Thermus genes. Phase III will proceed with commercializing the enzymes useful in human therapeutics and industrial applications.

描述：（改编自申请人摘要）。 调查人员 建议确定极端的完整基因组序列 嗜热菌Thermus flavus，并将基因功能分配给 以确定重要的商业活动。 ThermoGen的 染色体整合系统将用于验证功能 预测和研究与已知的缺乏同源性的ORF的表型 基因. 在第一阶段，他们将优化测序技术， 廉价和快速的测序数据，这个高GC基因组。 他们预计 测序150- 200 kb的栖热菌基因组， “喜鹊”自动化基因组调查环境，以找到ORF， 潜在的重要功能。 这些ORF的功能将是 实验验证。 在第二阶段，他们将完成生物体的序列， 大多数栖热菌基因的功能。 第三阶段将继续进行， 将可用于人类治疗和工业的酶商业化 应用.