Bitter Taste: Physiology and Behavior

苦味：生理和行为

• 批准号: 6682196
• 负责人: John D Boughter
• 金额: $ 7.15万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6682196
• 关键词: behavior test; behavioral /social science research tag; behavioral genetics; biological models; biological signal transduction; cell population study; chemoreceptors; cues; electrophysiology; epithelium; genetic strain; laboratory mouse; oral behavior; palate; psychophysiology; stimulus /response; taste; taste buds; tongue; voltage /patch clamp

DESCRIPTION (provided by applicant): Considerable variation exists among inbred strains of mice in their behavior toward bitter-tasting compounds. These differences have been demonstrated almost exclusively using long-term (24- or 48-hour) intake tests. Such natural variation in behavior has fostered physiological, biochemical, and molecular research aimed at elucidating taste transduction events and the identity of genes underlying the mechanisms of bitter taste. Two inbred strains exist that differ substantially in their intake of bitter stimuli: SWR/J (SW: bitter sensitive) and C3HeB/FeJ (C3: bitter insensitive). An understanding of how these strains differ physiologically could provide information useful for developing models of bitter taste function. However, very little is known about the responsiveness of taste receptor cells of these strains to bitter stimuli. Further, it is not certain that the long- term intake tests used to contrast these strains provide uncontaminated indicators of gustatory function. The approach taken in this application is to combine refined behavioral procedures with electrophysiological studies of taste receptor cells to elucidate the physiological bases of these behavioral differences. Behavioral measures from mice from both strains will be acquired using a short-term test designed to minimize non-taste (e.g., post-ingestive) factors. Precise concentration-response functions acquired in this way will provide a clear indication of the behavioral differences among bitter stimuli for these strains, based on taste cues. In electrophysiological experiments, taste receptor cells from the tongue and palate of these mice will be investigated using whole-cell-recording methods in an intact epithelial preparation. Cells will be tested with an array of bitter stimuli to test the hypothesis that the behavioral differences between strains are due to: 1) a greater number of cells responding to bitter stimuli in the bitter-sensitive SW strain; or 2) an altered concentration-response function for bitter stimuli in the bitter-insensitive C3 strain. By examining these strain differences in receptor cell function, these studies should help to link ongoing molecular advances to the neural mechanisms underlying bitter taste function.

描述（申请人提供）：近交系之间存在相当大的差异 不同品系的小鼠对苦味化合物的反应。这些 几乎完全使用长期（24或 48小时）摄入试验。这种行为上的自然变异 旨在阐明味觉的生理、生物化学和分子研究 转导事件和基因的身份背后的机制， 苦味。存在两种近交系，它们的摄入量差异很大 苦味刺激物：SWR/J（SW：苦味敏感）和C3 HeB/FeJ（C3：苦味 不敏感）。了解这些菌株在生理上的差异， 为开发苦味功能模型提供了有用的信息。 然而，人们对味觉感受器细胞的反应性知之甚少 对这些痛苦的刺激。此外，不确定长期- 用于对比这些菌株的长期摄入试验提供了未受污染的 味觉功能的指标。本申请中采用的方法是 联合收割机将精细的行为程序与味觉的电生理学研究相结合 受体细胞来阐明这些行为的生理基础 差异将获得两个品系小鼠的行为测量值 使用设计成最小化非味道的短期测试（例如，摄入后） 因素以这种方式获得的精确浓度响应函数将 提供了一个明确的指示行为之间的差异，痛苦的刺激， 根据味觉线索来区分这些菌株。在电生理学实验中， 这些小鼠舌头和上颚的味觉感受器细胞将被 在完整的上皮细胞中使用全细胞记录方法进行研究 准备.将用一系列苦味刺激测试细胞， 假设菌株之间的行为差异是由于：1）a 在对苦味敏感的SW中，有更多的细胞对苦味刺激作出反应 应变;或2）改变的浓度反应功能的苦味刺激， C3菌株。通过检查这些菌株的差异， 受体细胞功能，这些研究应该有助于连接正在进行的分子 苦味功能的神经机制研究进展。