In Vivo EPR Bioengineering Research Partnership

体内 EPR 生物工程研究合作伙伴

• 批准号: 6620064
• 负责人: Gareth R Eaton
• 金额: $ 30.03万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6620064
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; computer system design /evaluation; electron spin resonance spectroscopy; free radicals; interdisciplinary collaboration; laboratory mouse; magnetic field; mathematical model; model design /development

DESCRIPTION (provided by applicant): In vivo paramagnetic resonance (EPR) requires optimal signal-to-noise (S/N) enhancement in the shortest possible time. To study radicals in deep tissues it is necessary to perform the EPR measurements at low radiofrequency (e.g., 250 MHz), as in MRI. This decreases the S/N relative to the more common 9 GHz EPR. Physiological motions and metabolism occurring within the time of the usual EPR measurements necessitate the development of special techniques for in vivo studies. It is proposed to establish a partnership of engineers, research scientists, clinicians, and industry to fully engineer an EPR system dedicated to in vivo spectroscopy and imaging. As a first step, it is proposed to engineer a CW 250 MHz EPR spectrometer system optimized for the best in vivo free radical sensitivity per unit time. The specific tasks include the design, construction, and testing of an air-core magnet for in vivo EPR optimized for rapid magnetic field scans, and a control system for scanning the magnetic field rapidly. We introduce the innovation that the magnet will be resonated, and magnetic field scans will be sinusoidal. Measurement of the noise spectral densities of the spectrometer system, and of a spectrometer with a mouse in the resonator, will provide the basis for a mathematical model of the spectrometer noise characteristics from which one can predict the S/N per unit time expected for various magnetic field scan rates. The S/N for various scan rates will be compared with the predicted values. Software will be written to linearize and deconvolute the spectral information recorded under rapid-scan conditions. In subsequent effort it is proposed to extend the scope of the bioengineering research partnership to tackle the problems of optimal compensation for physiological motion, acquisition of the full RF spectrum and post-processing to replace analog pre-processing, and design of open magnet structures to achieve better patient acceptance and decrease costs.

描述（由申请人提供）：体内顺磁共振（EPR） 需要在尽可能短的时间内实现最佳信噪比（S/N）增强 时间为了研究深部组织中的自由基，必须进行EPR 在低射频下的测量（例如，250 MHz），如MRI。这降低 相对于更常见的9 GHz EPR的S/N。生理运动和 在通常的EPR测量时间内发生的代谢需要 开发用于体内研究的特殊技术。提出要 建立工程师、研究科学家、临床医生和 工业完全工程EPR系统专用于在体内光谱和 显像作为第一步，建议设计CW 250 MHz EPR 光谱仪系统优化最佳的体内自由基灵敏度， 单位时间具体任务包括设计、建造和测试 一种用于体内EPR的空芯磁体，其优化用于快速磁场扫描， 以及用于快速扫描磁场的控制系统。介绍了 创新，磁铁将共振，磁场扫描将 正弦曲线分光计噪声谱密度的测量 系统，以及谐振器中带有鼠标的光谱仪，将提供 光谱仪噪声特性的数学模型的基础， 可以预测不同磁场下单位时间的信噪比 扫描速率。将各种扫描速率的S/N与预测的 价值观将编写软件以线性化和去卷积光谱 在快速扫描条件下记录的信息。在随后的努力中， 建议扩大生物工程研究伙伴关系的范围， 解决生理运动的最佳补偿问题， 获取完整的射频频谱和后处理，以取代模拟 预处理和开放式磁体结构的设计，以实现更好的患者 接受并降低成本。