Rapid Scan Biomedical EPR Spectroscopy and Imaging

描述（由申请人提供）：将开发一种新的创新电子顺磁共振（EPR）方法，用于体内光谱学、成像和生物医学研究。工程师，研究科学家，临床医生和行业的合作伙伴关系，建议设计和建立快速扫描EPR系统在250 MHz和L波段（约。1.2 GHz）。重点是对光谱仪系统的精心设计，包括磁体、扫描线圈、谐振器和数据采集，以优化用于体内光谱学的生理EPR信号采集和在低RF频率下的成像，所述低RF频率被认为是体内动物研究的穿透深度和信噪比之间的最佳折衷。快速扫描EPR包括磁场扫描相对于弛豫时间快速的区域，这是CW和脉冲EPR之间的新开发的中间区域。直接检测快速扫描EPR信号直接提供吸收线型，揭示电子自旋弛豫时间，而不需要高入射功率，并提供准确的相对幅度的峰值在快速衰减的信号。具体目标是：（1）建立一个专用的L波段快速扫描光谱仪。1.2 GHz），扫描速率针对生物医学、体内和成像实验进行了优化。(2)在250 MHz下构建改进的谐振器、快速扫描线圈和驱动器，并优化快速扫描电桥。(3)在丹佛大学建立一个250 MHz的快速扫描桥，谐振器和磁场扫描线圈单元，并将其安装在芝加哥大学生理学体内成像中心，在那里它将用于成像动物肿瘤中的氧浓度。将比较传统CW成像、脉冲EPR成像和快速扫描成像在血氧测定和肿瘤学方面的受益。(4)设计、建造和测试硬件/软件系统，用于采集快速扫描信号和对将在250兆赫和L波段使用的频谱信息进行后处理。

DESCRIPTION (provided by applicant): A new and innovative electron paramagnetic resonance (EPR) methodology will be developed for in vivo spectroscopy, imaging and biomedical research. A partnership of engineers, research scientists, clinicians, and industry propose to design and build rapid-scan EPR systems operating at 250 MHz and at L-band (ca. 1.2 GHz). The emphasis is on careful engineering of the spectrometer systems, including magnet, scan coils, resonator, and data acquisition to optimize physiological EPR signal acquisition for in vivo spectroscopy and imaging at the low RF frequencies that are accepted as the best compromises between penetration depth and signal to noise for in vivo animal studies. Rapid-scan EPR encompasses the regime in which the magnetic field sweep is fast relative to relaxation times, which is a newly developed intermediate regime between CW and pulsed EPR. Direct-detection rapid-scan EPR signals provide the absorption lineshape directly, reveal electron spin relaxation times without requiring high incident power, and provide accurate relative amplitudes of peaks in rapidly decaying signals. The Specific Aims are: (1) Build a dedicated rapid-scan spectrometer at L-band (ca. 1.2 GHz) with scan rates optimized for biomedical, in vivo, and imaging experiments. (2) At 250 MHz build improved resonators, rapid-scan coils and drivers, and optimize the rapid-scan bridge. (3) Build a 250 MHz rapid-scan bridge, resonator, and magnetic field scan coil unit at the University of Denver and install it at the University of Chicago Center for In Vivo Imaging of Physiology, where it will be used to image oxygen concentrations in animal tumors. The benefits of traditional CW imaging, pulsed EPR imaging, and rapid-scan imaging for oximetry and oncology will be compared. (4) Design, build and test hardware/software systems for acquisition of the rapid-scan signal and post-processing of spectral information that will be used at 250 MHz and at L-band.