Rapid Scan Biomedical EPR Spectroscopy and Imaging

快速扫描生物医学 EPR 光谱和成像

• 批准号: 7434674
• 负责人: Gareth R Eaton
• 金额: $ 49.43万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7434674
• 关键词: Affect; Air; Animals; Biological Process; Biomedical Research; Chicago; Computer software; Computers; Detection; Digital Signal Processing; Disease; Electron Spin Resonance Spectroscopy; Electrons; Engineering; Free Radicals; Frequencies; Future; Image; Industry; Investigation; Measurement; Measures; Methodology; Methods; Mus; Noise; Operating System; Oxygen; Oxygen saturation measurement; Peripheral Vascular Diseases; Physiologic pulse; Physiological; Physiology; Process; Pulse taking; Rate; Relative (related person); Relaxation; Research; Research Personnel; Sampling; Scanning; Scientist; Signal Transduction; Source; Spectrum Analysis; Spin Trapping; System; Technology; Testing; Time; Universities; Wound Healing; absorption; cancer therapy; data acquisition; design; digital; experience; improved; in vivo; innovation; instrumentation; magnetic field; oncology; rapid detection; research study; response; software systems; tumor

A new and innovative electron paramagnetic resonance (EPR) methodology will be developed for in vivo spectroscopy, imaging and biomedical research. A partnership of engineers, research scientists, clinicians, and industry propose to design and build rapid-scan EPR systems operating at 250 MHz and at L-band (ca. 1.1 GHz). The emphasis is on careful engineering of the spectrometer systems, including magnet, scan coils, resonator, and data acquisition to optimize physiological EPR signal acquisition for in vivo spectroscopy and imaging. Rapid-scan EPR encompasses the regime in which the magnetic field sweep is fast relative to relaxation times, which is a newly developed intermediate regime between CW and pulsed EPR. Direct-detection rapid-scan EPR signals provide the absorption lineshape directly, reveal electron spin relaxation times without requiring high incident power, and provide accurate relative amplitudes of peaks in rapidly decaying signals. The Specific Aims are: (1) Build a dedicated rapid-scan spectrometer at L-band (ca. 1.1 GHz) with scan rates optimized for biomedical, in vivo, and imaging experiments. (2) Build improved resonators, rapid scan coils and drivers, and dedicated rapid-scan bridge at 250 MHz. (3) Build a 250 MHz rapid scan bridge, resonator, and magnetic field scan coil unit at the University of Denver and install it at the University of Chicago Center for In Vivo Imaging of Physiology, where it will be used to image oxygen concentrations in animal tumors. The benefits of traditional CW imaging, pulsed EPR imaging, and rapid-scan imaging for oximetry and oncology will be compared. (4) Design, build and test hardware/software systems for acquisition of the rapid-scan signal and post-processing of spectral information that will be used at 250 MHz and at L-band. In addition to in vivo imaging, future applications include the study of transient paramagnetic species, such as spin-trapped radicals with short lifetimes, time-dependent biological processes, and fundamental spin relaxation phenomena. This instrumentation is a new enabling technology and will create a new era for measuring oxygen in vivo and for scientific investigation of paramagnetic species, including in vivo free radicals. Free radicals are implicated in many diseases, and measurement of oxygen in vivo is important for cancer treatment, peripheral vascular disease, and wound healing.

一个新的和创新的电子顺磁共振（EPR）的方法将开发在体内 光谱学、成像和生物医学研究。工程师、研究科学家、临床医生， 和工业界提出设计和建造工作在250 MHz和L波段（约100 MHz）的快速扫描EPR系统。 1.1 GHz）。重点是对光谱仪系统的精心设计，包括磁铁，扫描 线圈、谐振器和数据采集，以优化体内生理EPR信号采集 光谱学和成像。快速扫描EPR涵盖了磁场扫描的区域， 快相对于弛豫时间，这是一个新开发的中间制度CW和脉冲 EPR。直接探测快速扫描EPR信号直接提供吸收线型，揭示电子自旋 弛豫时间，而不需要高入射功率，并提供精确的相对振幅的峰值， 快速衰减的信号 具体目标是：（1）建立一个专用的L波段快速扫描光谱仪。1.1 GHz），带扫描 针对生物医学、体内和成像实验优化的速率。(2)构建改进的谐振器，快速扫描 线圈和驱动器，以及250 MHz的专用快速扫描电桥。(3)搭建250 MHz快速扫描桥， 谐振器和磁场扫描线圈单元，并将其安装在丹佛大学， 芝加哥生理学体内成像中心，在那里它将被用来成像氧气浓度， 动物肿瘤传统CW成像、脉冲EPR成像和快速扫描成像的优点 将比较血氧测定法和肿瘤学。(4)设计、构建和测试硬件/软件系统， 采集快速扫描信号并对将在250 MHz下使用的频谱信息进行后处理 在L波段。除了在体成像，未来的应用还包括瞬态顺磁共振成像的研究。 物种，如短寿命的自旋捕获自由基，时间依赖性生物过程，以及 基本的自旋弛豫现象。这种仪器是一种新的使能技术， 测量体内氧和顺磁物质科学研究的新时代，包括 体内自由基自由基与许多疾病有关，体内氧的测量是重要的。 对于癌症治疗、外周血管疾病和伤口愈合很重要。