Rapid Scan Biomedical EPR Spectroscopy and Imaging

将开发一种新的创新电子顺磁共振（EPR）方法用于体内 光谱学、成像和生物医学研究。工程师、研究科学家、临床医生的合作伙伴关系， 业界建议设计和构建运行在 250 MHz 和 L 频段（约 100 MHz）的快速扫描 EPR 系统。 1.1 GHz）。重点是光谱仪系统的精心设计，包括磁铁、扫描 线圈、谐振器和数据采集，以优化体内生理 EPR 信号采集 光谱学和成像。快速扫描 EPR 涵盖了磁场扫描范围 相对于弛豫时间而言较快，这是一种新开发的介于连续波和脉冲之间的中间状态 EPR。直接检测快速扫描EPR信号直接提供吸收线形，揭示电子自旋 无需高入射功率即可获得弛豫时间，并提供准确的峰值相对振幅 快速衰减的信号。 具体目标是： (1) 构建一个具有扫描功能的 L 波段（约 1.1 GHz）专用快速扫描光谱仪 针对生物医学、体内和成像实验优化的速率。 (2) 构建改进的谐振器，快速扫描 线圈和驱动器，以及 250 MHz 的专用快速扫描桥。 (3) 搭建250 MHz快速扫描桥， 丹佛大学的谐振器和磁场扫描线圈单元，并将其安装在丹佛大学 芝加哥生理学体内成像中心，该中心将用于对体内氧气浓度进行成像 动物肿瘤。传统 CW 成像、脉冲 EPR 成像和快速扫描成像的优势 将比较血氧测定法和肿瘤学。 (4) 设计、构建和测试硬件/软件系统 采集快速扫描信号并对将在 250 MHz 下使用的光谱信息进行后处理 和在L波段。除了体内成像之外，未来的应用还包括瞬态顺磁的研究 物种，例如寿命短的自旋捕获自由基、时间依赖性生物过程，以及 基本的自旋弛豫现象。该仪器是一项新的使能技术，将创造一个 测量体内氧气和顺磁性物种科学研究的新时代，包括 体内自由基。自由基与许多疾病有关，体内氧的测量是 对于癌症治疗、周围血管疾病和伤口愈合很重要。

A new and innovative electron paramagnetic resonance (EPR) methodology will be developed for in vivo spectroscopy, imaging and biomedical research. A partnership of engineers, research scientists, clinicians, and industry propose to design and build rapid-scan EPR systems operating at 250 MHz and at L-band (ca. 1.1 GHz). The emphasis is on careful engineering of the spectrometer systems, including magnet, scan coils, resonator, and data acquisition to optimize physiological EPR signal acquisition for in vivo spectroscopy and imaging. Rapid-scan EPR encompasses the regime in which the magnetic field sweep is fast relative to relaxation times, which is a newly developed intermediate regime between CW and pulsed EPR. Direct-detection rapid-scan EPR signals provide the absorption lineshape directly, reveal electron spin relaxation times without requiring high incident power, and provide accurate relative amplitudes of peaks in rapidly decaying signals. The Specific Aims are: (1) Build a dedicated rapid-scan spectrometer at L-band (ca. 1.1 GHz) with scan rates optimized for biomedical, in vivo, and imaging experiments. (2) Build improved resonators, rapid scan coils and drivers, and dedicated rapid-scan bridge at 250 MHz. (3) Build a 250 MHz rapid scan bridge, resonator, and magnetic field scan coil unit at the University of Denver and install it at the University of Chicago Center for In Vivo Imaging of Physiology, where it will be used to image oxygen concentrations in animal tumors. The benefits of traditional CW imaging, pulsed EPR imaging, and rapid-scan imaging for oximetry and oncology will be compared. (4) Design, build and test hardware/software systems for acquisition of the rapid-scan signal and post-processing of spectral information that will be used at 250 MHz and at L-band. In addition to in vivo imaging, future applications include the study of transient paramagnetic species, such as spin-trapped radicals with short lifetimes, time-dependent biological processes, and fundamental spin relaxation phenomena. This instrumentation is a new enabling technology and will create a new era for measuring oxygen in vivo and for scientific investigation of paramagnetic species, including in vivo free radicals. Free radicals are implicated in many diseases, and measurement of oxygen in vivo is important for cancer treatment, peripheral vascular disease, and wound healing.