Ultrasonic Cryoconcentration of Human Plasma

人血浆的超声冷冻浓缩

• 批准号: 6645247
• 负责人: HOWARD E PURDUM
• 金额: $ 10万
• 依托单位: CRYOFACETS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2003-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6645247
• 关键词: animal tissue; clinical research; cryopreservation; cryoscience; freezing; human tissue; lyophilization; plasma; protein denaturation; technology /technique development; ultrasound

DESCRIPTION (provided by applicant): Plasma is of vital importance for direct transfusion and the manufacture of pharmaceuticals. Most plasma is frozen before such uses, however, during the freezing process, there is unavoidable, uncontrolled cryoconcentration of the plasma solutes The goal of this project is simply to control this universally accepted process. First, ultrasound is applied to enhance the formation and growth of ice crystals within the chilled plasma. Because the proteins are thus not frozen, they experience no mechanical freeze damage. Next, the salt concentrations are maintained at levels that do not denature the proteins. Compared to conventional systems, this new technology thus causes less damage to the plasma proteins, as shown by preliminary testing with cryoprecipitates. Most of the proposed experimental effort is devoted to determining the optimum operating conditions for this new technology. The parameters of greatest interest are the protein quality and yield versus time and applied ultrasonic intensity, over multiple sterile treatment bag geometries. Compared to conventional products, preliminary tests show that the resulting cryoconcentrates are cheaper to transport and store, they are easier to test for pathogens, and they have nearly twice the cryoprecipitate yield. They are also orders of magnitude faster to freeze, thaw, lyophilize and fractionate.

描述（由申请人提供）：血浆对于直接输血和药品生产至关重要。大多数血浆在这种用途之前被冷冻，然而，在冷冻过程中，存在不可避免的、不受控制的血浆溶质的冷冻浓缩。本项目的目标只是控制这个普遍接受的过程。首先，施加超声波以增强冷冻等离子体内冰晶的形成和生长。因为蛋白质因此不被冷冻，所以它们不经历机械冷冻损伤。接下来，将盐浓度维持在不使蛋白质变性的水平。与传统系统相比，这种新技术对血浆蛋白的损伤更小，正如冷沉淀物的初步测试所示。大多数拟议的实验工作致力于确定这种新技术的最佳操作条件。最感兴趣的参数是在多种无菌处理袋几何形状上的蛋白质质量和产量与时间和施加的超声强度的关系。与传统产品相比，初步测试表明，由此产生的冷冻浓缩物运输和储存成本更低，更容易检测病原体，并且其冷沉淀产量几乎是传统产品的两倍。它们的冷冻、解冻、冻干和破碎速度也快了几个数量级。