PATTERNING THE RETINA FOR COLOR VISION

为色觉设计视网膜图案

• 批准号: 6525143
• 负责人: Claude Desplan
• 金额: $ 37.8万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6525143
• 关键词: Drosophilidae; arthropod genetics; color visions; developmental genetics; developmental neurobiology; gene expression; genetic library; genetic screening; protein isoforms; retina; rhodopsin; sensory signal detection; visual pathways; visual perception; visual photoreceptor

DESCRIPTION (adapted from investigator's abstract): The Drosophila compound eye consists of 800 ommatidia, each of which contains two visual systems that perform different functions. The specific aims of the proposed experiments are: (1) Investigate the direction and timing of the signal between R7 and R8 that determines which pair of rh is expressed in a given ommatidium (p vs. y); (2) Test the model that the Rhodopsins themselves are able to influence the fate of a photoreceptor and its brain projection, and are also involved in the communication between R7 and R8; (3) characterize and identify genes specific to each subtype of photoreceptors, using p- and y-specific cDNA libraries and differential display; (4) Design a dominant genetic screen to identify factors responsible for the precise p/y ratio, using a technique to identify p or y ommatidia in live flies with GFP; and pursue a recessive mosaic screen to identify functions necessary for proper rh expression and for the communication between R7 and R8. This work may lead to an understanding of how communication among sensory receptor cells, as well as between these cells and the brain structures, allows establishment of the correct connections during development for processing sensory information.

描述（改编自研究者的摘要）：果蝇复眼 由 800 个小眼组成，每个小眼包含两个视觉系统 执行不同的功能。拟议实验的具体目标是： (1) 研究 R7 和 R8 之间信号的方向和时序 确定在给定小眼中表达哪对 rh（p 与 y）； (2) 测试视紫红质本身能够影响命运的模型 光感受器及其大脑投射，也参与 R7和R8之间的通信； (3) 表征和鉴定基因特异性 使用 p 和 y 特异性 cDNA 文库和 差分显示； (4) 设计显性遗传筛选来识别因素 负责精确的 p/y 比率，使用一种技术来识别 p 或 y 带有 GFP 的活果蝇小眼；并追求隐性马赛克屏幕 识别正确的 rh 表达和通讯所必需的功能 在R7和R8之间。这项工作可能会导致人们了解如何沟通 感觉受体细胞之间以及这些细胞和大脑之间 结构，允许在开发过程中建立正确的连接 用于处理感官信息。