Aging and rejuvenation: An ant model to study the regulation of longevity

衰老与返老还童：研究长寿调控的蚂蚁模型

• 批准号: 9925717
• 负责人: Claude Desplan
• 金额: $ 64.16万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9925717
• 关键词: Address; Adult; Age; Aging; Ants; Binding; Biological Models; Brain; Castes; Cells; Chromatin Structure; Clustered Regularly Interspaced Short Palindromic Repeats; Drosophila genus; Epigenetic Process; Exhibits; Fat Body; Female; Fostering; Gene Expression; Gene Expression Profile; Genes; Genetic; Genome; Growth; Individual; Insecta; Insulin; Insulin Antagonists; Insulin Receptor; Knock-out; Length; Longevity; MAP Kinase Gene; Mediating; Modeling; Molecular; Nurses; Organism; Ovary; Pheromone; Physiological; Physiology; Process; Proto-Oncogene Proteins c-akt; Regulation; Rejuvenation; Reproduction; Reproductive Behavior; Role; Signal Pathway; Signal Transduction; Site; Somatic Cell; System; Techniques; Telomerase; Testing; Tissues; cell type; differential expression; epigenetic regulation; histone modification; insulin signaling; insulin-like peptide; longevity gene; mRNA Expression; molecular phenotype; mutant; programs; reproductive; social; telomere; tool; transcriptome

Project summary: Ants are social insects that can be developed as experimentally tractable organisms for probing the dynamic changes in the epigenetic programs that control an array of processes, including aging. Aging is a process of progressive decline in intrinsic physiological functions. There is a well-established trade-off between lifespan and reproduction as higher reproductive activity in females is associated with shorter lifespan. However, in social insects, the reproductive queen has up to 10X longer lifespan than non-reproductive workers. In the ant Harpegnathos saltator, adult individuals that are not exposed to queen pheromones can undergo a reversible switch from non-reproductive workers to reproductive pseudo-queens (gamergates) that exhibit fully developed ovary and, importantly, a 5X increase in lifespan, showing that aging is reversible. Lifespan is shortened again when gamergates are reverted to workers (revertants). Thus, Harpegnathos provides an effective system to study epigenetic regulation of aging and rejuvenation given the adult plasticity that allows switching between castes. We have performed transcriptome analysis of the longevity-regulatory tissues: the fat body, ovary and brain, in workers vs. gamergates vs. revertants. We have identified a group of differentially expressed genes (DEGs), some of which have been implicated in the regulation of longevity, e.g. IIS (insulin and IGF signaling) pathway components. To further analyze the genetic and epigenetic regulation of longevity in ants, we will first compare physiology, lifespan, transcriptome and histone modifications in gamergates derived from young vs. old workers to ascertain the epigenetic regulation of aging and, most intriguingly, rejuvenation. The cellular localization and functions of important DEGs will be further analyzed. Second, we will utilize our newly established genetic tool – CRISPR in ants – to generate knockout (KO) ants in the two DEGs in the ovary, Hs- IMPL2 and Hs-ALS, which likely act as inhibitors of IIS in ants. These KO ants will be used to characterize the role of IIS in the dramatically extended lifespan in gamergates. Third, Hs-ILP1 (insulin-like peptide 1) is differentially expressed in the brain and Hs-ILP2 in the ovary. While both Hs-ILP1 and Hs-ILP2 are strongly increased in gamergates compared to workers, IIS is decreased in the fat body and ovary. To address this paradox, we will analyze (a) the role of Hs-ILP1 and -ILP2 in regulating the two branches of IIS: AKT and MAPK; (b) the role of Hs-ImpL2 and Hs-ALS in regulating activity of Hs-ILPs; and (c) how two insulin receptors (InRs) mediate the role of Hs-ILPs in differentially regulating IIS.

项目总结: