Conducting Biomaterials for Nerve Regeneration

用于神经再生的生物材料

• 批准号: 6883550
• 负责人: Silvia D Luebben
• 金额: $ 37.14万
• 依托单位: TDA RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6883550
• 关键词: biodegradable product; biomaterial compatibility; biomaterial development /preparation; electronic stimulator; electrophysiology; laboratory rat; medical implant science; nervous system regeneration; neural conduction; polymers; surface coating; tissue engineering; tissue support frame

DESCRIPTION (provided by applicant): In the U.S. more than one million people suffer serious peripheral nerve injuries every year and 20,000 procedures are carried out annually to repair severed peripherals nerves. Nerve guidance channels (NGCs) are hollow conduits used to bridge large gaps of severed peripheral nerves. Current NGC's materials have many drawbacks that often preclude their use or cause failure of the procedure. Therefore, new biomaterials that actively promote nerve regeneration are needed for the fabrication of improved NGCs. Electrically conducting biomaterials are of interest because they can be used to deliver electrical stimulation to responsive cells like osteoblasts, endothelial cells, and neurons. Polypyrrole (PPy) is an electrically conducting polymer that is not cytotoxic and has been demonstrated to favor the regeneration of damaged peripheral nerves in rats. Because PPy is a semiconductor, it can be used to deliver electric stimuli to cultured cells, accelerating their growth, migration and proliferation. Unfortunately PPy is not biodegradable, and NGCs made of PPy must be removed from the body upon healing, requiring a second surgery and causing additional site morbidity. Moreover, PPy, like most conducting polymers, is insoluble and infusible, and has poor mechanical properties. Therefore, it is very difficult to process it into the necessary form. Together, these properties limit the practical applications of PPy in tissue engineering. The objective of this project is to make a biodegradable form of PPy that can be processed from solution, and to test its properties as a support for the regeneration of severed nerves. During the Phase I project TDA successfully prepared a PPy-based biomaterials that are electrically conducting, can be processed from solution and support well neuron growth in vitro.

描述（由申请人提供）：在美国，每年有超过一百万人遭受严重的周围神经损伤，每年进行 20,000 例手术来修复切断的周围神经。神经引导通道（NGC）是一种空心导管，用于桥接切断的周围神经的大间隙。目前的 NGC 材料有许多缺点，常常妨碍其使用或导致手术失败。因此，需要积极促进神经再生的新生物材料来制造改进的 NGC。 导电生物材料引起人们的兴趣，因为它们可用于向成骨细胞、内皮细胞和神经元等响应细胞传递电刺激。聚吡咯 (PPy) 是一种无细胞毒性的导电聚合物，已被证明有利于大鼠受损周围神经的再生。由于 PPy 是一种半导体，因此可用于向培养细胞传递电刺激，加速其生长、迁移和增殖。不幸的是，PPy 不可生物降解，并且由 PPy 制成的 NGC 必须在愈合后从体内移除，需要进行第二次手术并导致额外的部位发病率。而且，PPy与大多数导电聚合物一样，不溶不熔，机械性能较差。因此，将其加工成所需的形式是非常困难的。这些特性共同限制了 PPy 在组织工程中的实际应用。 该项目的目标是制造一种可从溶液中加工的可生物降解形式的 PPy，并测试其作为切断神经再生支持物的特性。在第一阶段项目中，TDA 成功制备了一种基于 PPy 的生物材料，该材料具有导电性，可以从溶液中加工并支持良好的体外神经元生长。