Core--Eicosanoid Analysis Facility

核心——类二十烷酸分析设备

• 批准号: 6997743
• 负责人: JAMES R. MORROW
• 金额: $ 15.87万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-22 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6997743
• 关键词: analytical chemistry; animal tissue; biomedical facility; cancer prevention; chemoprevention; eicosanoids; human tissue; liquid chromatography mass spectrometry; method development; nonsteroidal antiinflammatory agent; prostaglandin E; prostaglandin endoperoxide synthase

The specific goals of the Eicosanoid Analysis Core are to carry out the analyses of eicosanoids in complex biological fluids and tissues for investigators in this Program Project Grant. The four projects proposed in this PPG involve research examining the role of the cyclooxygenase and eicosanoids in neoplasia and will require quantification of prostaglandins (PGs). Eicosanoids will be measured in cellular incubations and animal and human tissues by methodology employing either capillary gas chromatography (GC)/negative ion chemical ionization (NICI) mass spectrometry (MS) or electrospray ionization (ESI) MS using stable isotope dilution techniques that were developed by the Core Director and which are currently in use. Use of these assays by investigators involved in this PPG has allowed a number of fundamental observations to be made that have helped to delineate the role of eicosanoids in pathophysiological processes related to malignancy. In addition, the Core will also develop a ESl/tandem MS assay to quantify a major urinary metabolite of PGE2, 11alpha-hydroxy-9,15-dioxo-2,3,4,5,20-pentanor-19-carboxyprostanoic acid (PGE-M), in humans and rodents to define further the role of PGE2 in malignancy. The overall objectives of the Eicosanoid Core, therefore, are to provide technical support and implementation of highly sensitive methodology for the quantitative analysis of eicosanoids.

类花生酸分析核心的具体目标是进行以下分析： 在复杂的生物液体和组织中的类花生酸研究。本PPG中提出的四个项目涉及研究环氧合酶和类花生酸在肿瘤形成中的作用，并将需要定量测定前列腺素（PG）。将通过采用毛细管气相色谱（GC）/负离子化学电离（NICI）质谱（MS）或电喷雾电离（ESI）MS（使用核心总监开发的目前正在使用的稳定同位素稀释技术）的方法，在细胞孵育物以及动物和人体组织中测定类二十烷酸。参与本PPG的研究者使用这些测定法可以进行一些基本观察，这些观察有助于描述类二十烷酸在恶性肿瘤相关病理生理过程中的作用。此外，Core还将开发ESl/串联MS检测方法来定量PGE 2的主要尿液代谢物，11 α-羟基-9，15-二氧代-2，3，4，5，20-戊环-19-羧基前列腺烷酸（PGE-M）， 在人类和啮齿类动物中进一步确定PGE 2在恶性肿瘤中的作用。因此，类花生酸核心的总体目标是为类花生酸的定量分析提供技术支持和实施高度灵敏的方法。