Rapid In Situ Hybridization of DNA Repeats

DNA 重复序列的快速原位杂交

• 批准号: 6788940
• 负责人: PHILLIP T MOEN
• 金额: $ 17.66万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6788940
• 关键词: biopsy; biotechnology; cell line; diagnosis design /evaluation; fluorescent dye /probe; fluorescent in situ hybridization; high throughput technology; method development; natural gene amplification; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplastic growth; nucleic acid amplification techniques; nucleic acid probes; nucleic acid quantitation /detection; nucleic acid repetitive sequence; oligonucleotides; pathologic process; southern blotting

DESCRIPTION (provided by applicant): Changes to cellular DNA are often deleterious, leading to the development of malignant tumors. Alterations in cellular DNA can accumulate as point mutations, deletions, inversions, translocations or sequence amplification. Sequence amplification is particularly common and has been detected in as much as 34% of breast cancers. It is often associated with advanced disease and poor prognosis. Detection of amplified genes within tumors is vital to determining patient outlook and treatment course. Fluorescence in situ hybridization (FISH) is a powerful tool for detecting nucleic acids in morphologically intact cells, however, assay sensitivity continues to be a significant obstacle to widespread usage. Improved diagnostic methods are needed to understand cancer pathogenesis and determine proper therapeutic intervention. This Small Business Innovation Research Phase I project aims to develop a sensitive, rapid, reproducible and cost effective FISH assay for detecting gene amplification in cancer biopsies.

描述（由申请人提供）：细胞DNA的变化通常是有害的，导致恶性肿瘤的发展。细胞DNA中的改变可以累积为点突变、缺失、倒位、易位或序列扩增。序列扩增特别常见，在多达34%的乳腺癌中检测到。它通常与晚期疾病和预后不良有关。肿瘤内扩增基因的检测对于确定患者的前景和治疗过程至关重要。荧光原位杂交（FISH）是用于检测形态完整细胞中的核酸的有力工具，然而，测定灵敏度仍然是广泛使用的显著障碍。需要改进的诊断方法来了解癌症发病机制并确定适当的治疗干预。该小型企业创新研究I期项目旨在开发一种灵敏、快速、可重复和具有成本效益的FISH检测方法，用于检测癌症活检中的基因扩增。