RNA editing of AMPA receptor subunit GluR2 in ischemia

缺血中 AMPA 受体亚基 GluR2 的 RNA 编辑

基本信息

  • 批准号:
    7233658
  • 负责人:
  • 金额:
    $ 31.02万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2006
  • 资助国家:
    美国
  • 起止时间:
    2006-05-15 至 2011-01-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Ischemic stroke is the third leading cause of death in developed countries. A critical feature of the disease is a highly selective pattern of neuronal loss; certain identifiable subsets of neurons, particularly CA1 pyramidal neurons in the hippocampus, are severely damaged while others remain intact. A step in this selective neuronal injury involves Ca2+ entry through Ca2+-permeable AMPA receptor channels. AMPA receptors are a major subtype of glutamate receptors (GluRs) that are assembled from GluR1-4 subunits. Ca2+ permeability of the channels is dominated by GluR2 RNA editing at the Q/R site; edited GluR2(R) subunits form Ca2+-impermeable channels, whereas unedited GluR2(Q) channels allow Ca2+ entry. In most CA1 neurons, AMPA receptor channels contain GluR2(R), and thus are impermeable to Ca2+ flow. Recently, we have identified that transient forebrain ischemia selectively disrupts GluR2 Q/R site editing and hence induces injurious Ca2+ entry through AMPA receptor channels into vulnerable CA1 neurons. We have also shown that impaired GluR2 Q/R site editing is closely correlated with reduced expression of ADAR2 (short for adenosine deaminase acting on RNA) gene, a nuclear enzyme responsible for GluR2 Q/R site editing. We thus hypothesize that reduced expression of ADAR2 gene is responsible for the impaired GluR2 Q/R site editing. To address this hypothesis directly, we will determine if restoration of ADAR2 gene expression rescues GluR2 Q/R site editing and in turn blocks Ca2+ permeability of AMPA receptor channels, leading to the survival of vulnerable neurons in the post-ischemic rats. Overall, this project will have two specific aims: Specific Aim 1: To determine whether restoration of ADAR2 gene expression blocks Ca2+ entry through AMPA receptor channels and rescues vulnerable neurons in the post-ischemic rats. Specific Aim 2: To determine if generation of stable ADAR2 gene silencing induces degeneration of ischemia-insensitive neurons, and if degeneration of ADAR2-deficient neurons results from RNA editing deficits of one or more glutamate receptor subunits. Together, this project will identify that ADAR2-dependent GluR2 Q/R site editing determines vulnerability of neurons to ischemia. Thus, this work will define a promising target for stoke therapy.
描述(申请人提供):在发达国家,缺血性中风是第三大主要死亡原因。这种疾病的一个关键特征是高度选择性的神经元丢失模式;某些可识别的神经元亚群,特别是海马区的CA1锥体神经元,严重受损,而其他神经元保持完好。这种选择性神经元损伤的一个步骤是通过钙离子渗透性的AMPA受体通道进入钙离子。AMPA受体是谷氨酸受体(GluRs)的主要亚型,由GluR1-4亚基组装而成。通道的钙通透性主要由Q/R处的GluR2 RNA编辑决定;编辑后的GluR2(R)亚基形成不透钙通道,而未编辑的GluR2(Q)通道允许钙内流。在大多数CA1神经元中,AMPA受体通道含有GluR2(R),因此对钙离子流动是不通透的。最近,我们发现短暂性前脑缺血选择性地干扰GluR2 Q/R位点的编辑,从而诱导损伤性钙离子通过AMPA受体通道进入脆弱的CA1神经元。我们还发现,GluR2 Q/R位点编辑受损与负责GluR2 Q/R位点编辑的核酶ADAR2(腺苷脱氨酶作用于RNA)基因的表达减少密切相关。因此,我们推测ADAR2基因的表达减少是导致GluR2 Q/R位点编辑受损的原因。为了直接解决这一假说,我们将确定ADAR2基因表达的恢复是否挽救了GluR2 Q/R位点的编辑,进而阻止AMPA受体通道的钙通透性,导致缺血后大鼠易受伤害神经元的存活。总体而言,本项目将有两个具体目标:具体目标1:确定ADAR2基因表达的恢复是否阻止通过AMPA受体通道的钙内流,并拯救缺血后大鼠易受伤害的神经元。具体目的2:确定稳定的ADAR2基因沉默是否导致缺血不敏感神经元的变性,以及ADAR2缺陷神经元的变性是否是由于一个或多个谷氨酸受体亚基的RNA编辑缺陷所致。总之,该项目将确定ADAR2依赖的GluR2 Q/R位点编辑决定了神经元对缺血的脆弱性。因此,这项工作将为卒中治疗定义一个有前景的靶点。

项目成果

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YOUMING LU其他文献

YOUMING LU的其他文献

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{{ truncateString('YOUMING LU', 18)}}的其他基金

DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    7675965
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    7888146
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    7522367
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    8142986
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    7786536
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
DAPK1 regulation of NMDA receptors in ischemic neuronal death
DAPK1 对 NMDA 受体在缺血性神经元死亡中的调节
  • 批准号:
    8117740
  • 财政年份:
    2008
  • 资助金额:
    $ 31.02万
  • 项目类别:
RNA editing of AMPA receptor subunit GluR2 in ischemia
缺血中 AMPA 受体亚基 GluR2 的 RNA 编辑
  • 批准号:
    7760657
  • 财政年份:
    2006
  • 资助金额:
    $ 31.02万
  • 项目类别:
RNA editing of AMPA receptor subunit GluR2 in ischemia
缺血中 AMPA 受体亚基 GluR2 的 RNA 编辑
  • 批准号:
    7766886
  • 财政年份:
    2006
  • 资助金额:
    $ 31.02万
  • 项目类别:
RNA editing of AMPA receptor subunit GluR2 in ischemia
缺血中 AMPA 受体亚基 GluR2 的 RNA 编辑
  • 批准号:
    7140760
  • 财政年份:
    2006
  • 资助金额:
    $ 31.02万
  • 项目类别:
RNA editing of AMPA receptor subunit GluR2 in ischemia
缺血中 AMPA 受体亚基 GluR2 的 RNA 编辑
  • 批准号:
    7354764
  • 财政年份:
    2006
  • 资助金额:
    $ 31.02万
  • 项目类别:

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