Next Generation Sequencing Core

下一代测序核心

• 批准号: 10714179
• 负责人: ERLE S. ROBERTSON
• 金额: $ 45.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10714179
• 关键词: ATAC-seq; Address; Award; Bioinformatics; Biological Assay; Biometry; Cancer Center; Center Core Grants; ChIP-seq; Complement; Complex; Core Facility; DNA Sequencing Facility; DNA sequencing; Data; Data Analyses; Genetic Transcription; Genomics; Goals; Herpesviridae; High-Throughput Nucleotide Sequencing; Human Genome; Human Herpesvirus 4; Human Herpesvirus 8; Hypoxia; Leadership; Libraries; Maps; Methodology; Methods; Oncogenic; Oncogenic Viruses; Online Systems; Preparation; Procedures; Protocols documentation; Research Personnel; Sampling; Services; Standardization; Technical Expertise; Technology; Work; chromatin immunoprecipitation; data management; experience; experimental study; follow-up; medical schools; member; next generation sequencing; programs; success; transcriptome sequencing

Summary Core C: Next Generation Sequencing core (NGSC) The next generation sequencing core (NGSC) will be responsible for all next generation sequencing activities by the four projects. All sequencing activities will be performed in Core C. RNA-Seq, ChIP-Seq, ATAC-Seq, Core C will be responsible for 1) performing NGS-assays; 2) generating library for Illumina next-generation sequencing, 3) mapping sequence data to EBV, KSHV, MPyV and human genomes, and 4) providing all follow-up bioinformatics and biostatistics analyses for the overall program. These goals will be accomplished in conjunction with the Penn Genomics, Next-Gen-Sequencing and Bioinformatics Cores, which provides Illumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a password protected shared web-based browser for program investigators. Expertise in all aspects of Next-Gen-Sequencing (NGS), Chromatin immunoprecipitation, and Bioinformatics support will be provided to the projects for the lifetime of the award. The NGS core will be led by experience leaders in administration and technical expertise to ensure that the core will be successful. Dr. Lieberman, is an expert in NGS and is familiar with all aspects of NGS technologies. Dr. Robertson has led many large complex programs, and is familiar with management and coordination of the technical expertise needed for success. Thus, the leadership is familiar with different NGS methodologies and bioinformatics analyses as applied to oncogenic herpesviruses and the human genome. Core C leaders have both provide unique expertise that complement each other. The NGS Core C will facilitate the ability of all members of the Program to standardize methods for sample preparation and for post-sequence bioinformatics analyses. The work will be performed at the Perelman School of Medicine in space adjacent to the Robertson lab, and in conjunction with the Penn Genomics NGS Core, and Bioinformatics core supported in part by the Abramson Cancer Center.

摘要 核心C：下一代测序核心(NGSC) 下一代测序核心(NGSC)将负责所有下一代测序活动 这四个项目。所有测序活动将在Core C.RNA-Seq、ChIP-Seq、ATAC-Seq、 核心C将负责1)执行NGS分析；2)为Illumina下一代生成文库 测序，3)将序列数据映射到EBV、KSHV、MPyV和人类基因组，以及4)提供所有 对整个项目进行后续生物信息学和生物统计分析。这些目标将在#年实现。 与宾夕法尼亚大学基因组学、下一代测序和生物信息学核心合作，提供Illumina 将下一代测序作为核心服务。最终数据管理和分析将可供访问 通过为项目调查员提供的受密码保护的共享网络浏览器发送到每个项目调查员。 精通下一代测序(NGS)、染色质免疫沉淀和生物信息学的方方面面 在奖项的有效期内，将向项目提供支持。NGS核心将由经验引领 行政领导和技术专长，以确保核心将是成功的。利伯曼博士，是 NGS专家，熟悉NGS技术的方方面面。罗伯逊博士领导了许多大型 复杂的计划，并熟悉管理和协调所需的技术专业知识 成功。因此，领导层熟悉不同的NGS方法和生物信息学分析，如 应用于致癌疱疹病毒和人类基因组。核心C领导者都提供了独特的 专业知识相辅相成。NGS核心C将促进计划所有成员的能力 标准化样品制备和序列后生物信息学分析的方法。这项工作将 在毗邻罗伯逊实验室的佩雷尔曼医学院进行，并与 宾夕法尼亚大学基因组学NGS核心，以及部分由艾布拉姆森癌症支持的生物信息学核心 中心。