Next Generation Sequencing Core

总结 核心C：下一代测序核心（NGSC） 下一代测序核心（NGSC）将负责所有下一代测序活动 四个项目。所有测序活动将在核心C中进行。RNA-Seq，ChIP-Seq，ATAC-Seq， 核心C将负责1）执行NGS测定; 2）为Illumina下一代产生文库 测序，3）将序列数据映射到EBV、KSHV、MPyV和人基因组，以及4）提供所有的 对整个项目进行后续生物信息学和生物统计学分析。这些目标将在 与Penn Genomics，Next-Gen-Sequencing和Bioinformatics Cores合作，提供Illumina 下一代测序作为核心服务。将提供最终数据管理和分析 通过受密码保护的共享网络浏览器向每个项目调查员提供。 在下一代测序（NGS）、染色质免疫沉淀和生物信息学的各个方面都有专业知识 资助计划将在奖项有效期内持续提供资助。NGS核心将由经验主导 领导人在行政和技术专长，以确保核心将是成功的。利伯曼医生 NGS专家，熟悉NGS技术的各个方面。罗伯逊博士领导了许多大型 复杂的程序，并熟悉管理和协调所需的技术专长， 成功因此，领导层熟悉不同的NGS方法和生物信息学分析， 应用于致癌疱疹病毒和人类基因组。核心C领导者都提供了独特的 专业知识相辅相成。NGS核心C将促进该计划的所有成员的能力 标准化样品制备和测序后生物信息学分析的方法。这项工作将 在佩雷尔曼医学院与罗伯逊实验室相邻的太空中进行， 与Penn Genomics NGS核心，以及部分由Abramson Cancer支持的生物信息学核心 中心

Summary Core C: Next Generation Sequencing core (NGSC) The next generation sequencing core (NGSC) will be responsible for all next generation sequencing activities by the four projects. All sequencing activities will be performed in Core C. RNA-Seq, ChIP-Seq, ATAC-Seq, Core C will be responsible for 1) performing NGS-assays; 2) generating library for Illumina next-generation sequencing, 3) mapping sequence data to EBV, KSHV, MPyV and human genomes, and 4) providing all follow-up bioinformatics and biostatistics analyses for the overall program. These goals will be accomplished in conjunction with the Penn Genomics, Next-Gen-Sequencing and Bioinformatics Cores, which provides Illumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a password protected shared web-based browser for program investigators. Expertise in all aspects of Next-Gen-Sequencing (NGS), Chromatin immunoprecipitation, and Bioinformatics support will be provided to the projects for the lifetime of the award. The NGS core will be led by experience leaders in administration and technical expertise to ensure that the core will be successful. Dr. Lieberman, is an expert in NGS and is familiar with all aspects of NGS technologies. Dr. Robertson has led many large complex programs, and is familiar with management and coordination of the technical expertise needed for success. Thus, the leadership is familiar with different NGS methodologies and bioinformatics analyses as applied to oncogenic herpesviruses and the human genome. Core C leaders have both provide unique expertise that complement each other. The NGS Core C will facilitate the ability of all members of the Program to standardize methods for sample preparation and for post-sequence bioinformatics analyses. The work will be performed at the Perelman School of Medicine in space adjacent to the Robertson lab, and in conjunction with the Penn Genomics NGS Core, and Bioinformatics core supported in part by the Abramson Cancer Center.