ACTIVITY #1 - PROTEOMICS DISCOVERY FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质组学是一个新兴的研究领域，已经成功地应用于识别疾病进展的细胞生物标记物。密码蛋白质芯片技术是蛋白质组学平台的重要组成部分，可在与高效液相、FPLC和LC/MS/MS一起用于蛋白质鉴定时，快速发现多个生物标记物。统计/生物信息学也是与内布拉斯加州大学医学中心(UNMC)专家合作在波多黎各大学医学校区(UPR MSC)建立的蛋白质组学平台的补充。这一核心基金的长期目标是通过在蛋白质组学方面建立必要的平台和专业知识，以及与生物标记物发现相关的基础和临床项目，来增强我们的机构生物医学研究能力。提出了以下具体目标：1.为鉴定和分离健康差异人群中差异表达的蛋白质提供必要的基础设施。2.提高UPR-MSC教职员工和研究生开展蛋白质组学研究项目的能力。3.为教师和学生提供最先进的蛋白质组核心实验室，并根据每个生物系统使用不同的方法提供蛋白质组分析的建议、服务和培训。4.通过促进内地生物统计学家的合作，为蛋白质鉴定和分析机构做好准备。这一研究基础设施将由UPR-MSC研究人员使用，他们已经开始在试点项目中使用SELDI-TOF，这些项目在艾滋病毒疾病、癌症、糖尿病、心血管疾病、遗传学和传染病领域影响我们的人口。由于蛋白质发现需要复杂的设备，我们建议开发一个拥有最少必要仪器和技术专长的蛋白质组学核心设施，为UPR-MSC的研究人员提供途径并提高他们的生产率。这项活动的意义在于，通过加强我们在影响少数群体人口、包括妇女的疾病方面的生物医学研究能力，缩小健康差距。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics is an emerging area of research that has been applied successfully to identify cellular biomarkers of disease progression. Ciphergens ProteinChip¿ technology is an important component of the proteomics platform for the rapid discovery of multiple biomarkers when used with HPLC, FPLC and LC/MS/MS for identification of proteins. Statistics/Bioinformatics is also necessary to complement the proteomics platform that has been established at University of Puerto Rico Medical Sciences Campus (UPR MSC) in collaboration with experts at University of Nebraska Medical Center (UNMC). The long term goal of this Core Facility is to enhance our institutional biomedical research capability by establishing the necessary platform and expertise in proteomics to basic and clinical projects related to biomarker discovery. The following specific aims are proposed: 1. To provide the infrastructure necessary to characterize, and isolate differentially expressed proteins in populations with health disparities. 2. To increase the capabilities of performing research projects in proteomics among faculty and graduate students at the UPR-MSC. 3.To maintain a state of the art proteomic Core laboratory available for faculty and students and provide advice, service and training of proteomics analyses using diverse approaches in accordance to each biological system. 4.To prepare the institution for protein identification and analysis by fostering collaboration of the Biostatisticians in the mainland. This research infrastructure will be used by the UPR-MSC investigators who have started using the SELDI-TOF in pilot projects that affect our population in the areas of HIV Disease, Cancer, Diabetes, Cardiovascular diseases, Genetics and Infectious diseases. Since Protein Discovery requires sophisticated equipments, we propose to develop a Proteomics Core Facility with the minimum necessary instruments and technical expertise to provide access to investigators at UPR-MSC and increase their productivity. The relevance of this Activity is the reduction in Health Disparities by enhancing our biomedical research capability in the diseases that affect minority populations including women.