ACTIVITY #1 - PROTEOMICS DISCOVERY FACILITY

活动

• 批准号: 7715296
• 负责人: LOYDA M MELENDEZ
• 金额: $ 39.81万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715296
• 关键词: Affect; Area; Arts; Bioinformatics; Biological; Biological Markers; Biomedical Research; Cardiovascular Diseases; Clinical; Collaborations; Communicable Diseases; Complement; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Diabetes Mellitus; Disease; Disease Progression; Equipment; Faculty; Fostering; Funding; Genetic; Goals; Grant; HIV; High Pressure Liquid Chromatography; Institution; Laboratories; Malignant Neoplasms; Medical; Medical center; Minority; Nebraska; Pilot Projects; Population; Productivity; Proteins; Proteomics; Puerto Rico; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Science; Services; Source; Students; System; Technical Expertise; Technology; Training; United States National Institutes of Health; Universities; Woman; fast protein liquid chromatography; health disparity; instrument; statistics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics is an emerging area of research that has been applied successfully to identify cellular biomarkers of disease progression. Ciphergen's ProteinChip¿ technology is an important component of the proteomics platform for the rapid discovery of multiple biomarkers when used with HPLC, FPLC and LC/MS/MS for identification of proteins. Statistics/Bioinformatics is also necessary to complement the proteomics platform that has been established at University of Puerto Rico Medical Sciences Campus (UPR MSC) in collaboration with experts at University of Nebraska Medical Center (UNMC). The long term goal of this Core Facility is to enhance our institutional biomedical research capability by establishing the necessary platform and expertise in proteomics to basic and clinical projects related to biomarker discovery. The following specific aims are proposed: 1. To provide the infrastructure necessary to characterize, and isolate differentially expressed proteins in populations with health disparities. 2. To increase the capabilities of performing research projects in proteomics among faculty and graduate students at the UPR-MSC. 3.To maintain a state of the art proteomic Core laboratory available for faculty and students and provide advice, service and training of proteomics analyses using diverse approaches in accordance to each biological system. 4.To prepare the institution for protein identification and analysis by fostering collaboration of the Biostatisticians in the mainland. This research infrastructure will be used by the UPR-MSC investigators who have started using the SELDI-TOF in pilot projects that affect our population in the areas of HIV Disease, Cancer, Diabetes, Cardiovascular diseases, Genetics and Infectious diseases. Since Protein Discovery requires sophisticated equipments, we propose to develop a Proteomics Core Facility with the minimum necessary instruments and technical expertise to provide access to investigators at UPR-MSC and increase their productivity. The relevance of this Activity is the reduction in Health Disparities by enhancing our biomedical research capability in the diseases that affect minority populations including women.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 蛋白质组学是一个新兴的研究领域，已成功应用于确定疾病进展的细胞生物标志物。Ciphergen的蛋白质芯片¶该技术是蛋白质组学平台的重要组成部分，当与HPLC、FPLC和LC/MS/MS一起用于鉴定蛋白质时，可快速发现多种生物标志物。统计学/生物信息学也是必要的，以补充蛋白质组学平台，该平台已在波多黎各大学医学科学校区（UPR MSC）与内布拉斯加大学医学中心（UNMC）的专家合作建立。这个核心设施的长期目标是通过建立必要的平台和蛋白质组学的专业知识，以提高我们的机构生物医学研究能力，以基础和临床项目相关的生物标志物发现。提出了以下具体目标：1。提供必要的基础设施来表征和分离健康差异人群中差异表达的蛋白质。2.提高UPR-MSC教师和研究生在蛋白质组学方面进行研究项目的能力。3.保持最先进的蛋白质组核心实验室可供教师和学生，并提供建议，服务和培训的蛋白质组学分析使用不同的方法，根据每个生物系统。4.促进国内生物统计学家的合作，为蛋白质鉴定和分析机构做好准备。该研究基础设施将由UPR-MSC研究人员使用，他们已经开始在影响我们人群的艾滋病，癌症，糖尿病，心血管疾病，遗传学和传染病领域的试点项目中使用SELDI-TOF。由于蛋白质发现需要先进的设备，我们建议开发一个蛋白质组学核心设施，配备最少的必要仪器和技术专长，为UPR-MSC的研究人员提供访问机会，并提高他们的生产力。这项活动的意义在于通过加强我们对影响包括妇女在内的少数民族人口的疾病的生物医学研究能力，减少健康差距。