Expansion of Macaque Breeding runs at the New Iberia Research Center

路易斯安那大学拉斐特分校C 06 新伊比利亚研究中心猕猴繁殖能力的扩大 摘要： 作为其艾滋病毒/艾滋病研究计划的扩展的一部分，新的伊比利亚研究中心（NIRC）， 路易斯安那大学拉斐特分校（UL Lafayette）建议通过 建造2栋多人住房，配有屋顶、水电、暖气和空气流通设施 通过Ragin Cajun Facilities，Inc.基金会 NIRC在过去6年中经历了指数级增长，在SARS-CoV 2大流行中发挥了关键作用 同时保持一个积极的艾滋病研究项目，以支持那些实验室被关闭的合作者 下来作为这一增长的一部分，NIRC已经扩大了其非人灵长类动物（NHP）的繁殖和队列，从6400 到超过10，000个NHP，现在是美国最大的灵长类动物中心。最近中国暂停灵长类动物出口 以及NHP在货物运输中的局限性，造成了研究的严重和巨大短缺 NHPs因大量用于测试COVID疫苗和疗法而显著放大。这已经增加了一倍和三倍 动物获取成本，这将在很大程度上限制学术研究人员对灵长类动物的研究， 十年及以后。在努力缓解这一供应缺口，NIRC已经并将继续扩大NHP育种 除了正在进行的实验室空间翻新和BSL-3的建设外， 重新分组啮齿类动物国家卫生和计划署和实验室育种和研究空间的不断增加是 将原来用于黑猩猩的空间转换为猴子的社交和研究（四笼）住房， 以及在2021年获得的C 06奖项的支持下建造2栋新的多功能住宅楼（预计 完成和入住日期为2023年底）。NIRC目前有~1400只2-4岁的恒河猴未来 饲养者预定占据这个空间，并考虑到每年超过1000只恒河猴的活产率，NIRC已经 进一步扩大这种住房选择，通过本提案建造另外2个相同的单元。的 请注意，在同一地区将有空间增加3个这样的单位，以便将来扩展。这种类型的建筑有 在德克萨斯州的一个设施中专门设计和广泛测试猕猴住房/繁殖，证明 在南方气候中，这种结构的适当性、易于维护性和寿命。每个建筑物 一个单元将包括20个室内/室外跑道，每个跑道能够容纳20只种畜，总容量为 约800个繁殖者和他们的后代这种扩展对于适应快速增长的研究至关重要 国家艾滋病研究中心的艾滋病毒/艾滋病方案以及国家艾滋病研究所的艾滋病毒/艾滋病研究中心和SVEU的艾滋病毒/艾滋病重点方案。NIRC支持 许多关于慢病毒发病机制的现场和合作项目都得到了最先进成像技术的支持 能力包括全身信号细胞分析方法，旨在治愈艾滋病毒的治疗方法， 以及通过保护性实验性疫苗、阴道疫苗和 结直肠杀微生物剂。由于NHP和空间短缺，这些项目中的许多项目都出现了延误， 这阻碍了NIRC的科学扩展和对NIH资助的HIV/AIDS研究的支持。

University of Louisiana at Lafayette C06 Expansion of Macaque Breeding capacity at the New Iberia Research Center Abstract: As part of its expansion of its HIV/AIDS research program, the New Iberia Research Center (NIRC) at the University of Louisiana at Lafayette (UL Lafayette) proposes to add macaque breeding capacity by the construction of 2 multi-run housing buildings complete with roofing, electricity, water, heating and air circulation for an added capacity of 1600 macaque breeders + offsprings via the Ragin Cajun Facilities, Inc. Foundation. NIRC has experienced exponential growth in the past 6 years, playing a critical role in the SARS-CoV2 pandemic while maintaining a vigorous HIV research program in support of collaborators whose laboratories were shut down. As part of this growth, NIRC has expanded its nonhuman primate (NHP) breeding and cohorts, from 6,400 to >10,000 NHPs, now the largest primate center in the US. The recent suspension of primate export from China and the limitations on NHP transport in cargo, have created an acute and monumental shortage of research NHPs, markedly amplified by their heavy use to test COVID vaccines and therapies. This has doubled and tripled animal acquisition costs, which will largely limit primate based research for academic investigators for the next decade and beyond. In efforts to palliate this gap in supply, NIRC has, and continues to expand NHP breeding and research housing, in addition to ongoing renovation of laboratory space and the construction of a BSL-3 facility regrouping rodents, NHPs and labs. The ongoing increase in breeder and research space has been the conversion of space originally used for chimpanzees into monkey social and research (quad cages) housing, and the construction of 2 new multi-run housing buildings supported by a C06 award obtained in 2021 (projected completion and occupancy date in late 2023). NIRC currently has ~1400 2-4 year old rhesus macaques future breeders slated to occupy this space, and given an annual live birth rate of >1000 rhesus macaques, NIRC has to expand such housing option further, via the construction of 2 additional identical units via this proposal. Of note, there will be space for 3 more such units for future expansion in the same area. This type of building has been specifically designed and extensively tested for macaque housing/breeding at a facility in Texas, attesting to the adequacy, ease of maintenance and longevity of such construction in southern climates. Each building unit will comprise 20 indoor/outdoor runs able to accommodate up to 20 breeder animals each for a total capacity of ~800 breeders and their offsprings. This expansion is critical to accommodate the rapidly increasing research programs at NIRC as well as the support of NIAID VRC and SVEU HIV/AIDS focused programs. NIRC supports many both onsite and collaborative programs on lentiviral pathogenesis supported by state of the art imaging capabilities including a whole body to signal cell analysis approach, therapeutic approaches aimed at curing HIV, and an extensive program to prevent mucosal viral acquisition via protective experimental vaccines, vaginal and colorectal microbicides. Many of these programs are experiencing delays due to NHP and space shortages, which are holding back the scientific expansion of NIRC and the support of HIV/AIDS NIH funded research.