Untethered high channel count electrophysiology for freely-moving animals

适用于自由活动动物的不受束缚的高通道数电生理学

• 批准号: 10761109
• 负责人: John L Sherwood
• 金额: $ 51.98万
• 依托单位: LEAFLABS, LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10761109
• 关键词: Address; Alzheimer' s Disease; Animal Behavior; Animals; Architecture; BRAIN initiative; Behavior; Behavioral; Bluetooth; Brain; Brain Diseases; Cells; Cognition; Complex; Custom; Data; Development; Electrodes; Electrophysiology (science); Engineering; Ensure; Environment; Epilepsy; Funding; Generations; Goals; Head; Image; Individual; Learning; Mammals; Memory; Mental Depression; Neurobiology; Neurofibrillary Tangles; Neurologic; Neurons; Noise; Parkinson Disease; Performance; Peripheral; Phase; Pilot Projects; Population; Production; Research Personnel; Resolution; Rodent; Sampling; Scientific Inquiry; Services; Silicon; Small Business Innovation Research Grant; Sorting; Stream; Surface; System; Techniques; Technology; Telemetry; Testing; Thinness; Time; Tissues; Weight; Willow; Wireless Technology; Writing; analog; behavioral study; cost; data acquisition; design; digital; driving force; effective therapy; experimental study; improved; in vivo; innovation; light weight; manufacture; millisecond; neural; neural circuit; neural stimulation; neuronal circuitry; novel; power consumption; real time monitoring; research facility; sensor; theories; tool; wireless; wireless fidelity

PROJECT SUMMARY Recording whole-brain activity with single neuron resolution and millisecond timescale precision is crucial to understanding how individual cells and complex neural circuits interact in both time and space. Simultaneous recording and stimulation of large populations of neurons distributed throughout the brain are needed to rigorously evaluate theories of neural computation at the cellular level in mammals, and extended longitudinal recordings are required to establish general principles for neuronal circuits/dynamics and how complex neuronal activity relates to behavior, both to further our fundamental understanding of the brain, but also to surface underlying causes of neurological and psychiatric conditions such as Alzheimer’s, Parkinson’s, TBI, epilepsy, and depression, and to aid development of novel and more effective treatments. These are key goals of the BRAIN initiative, and the driving force behind LeafLabs' Willow, an electrophysiology recording system designed to take advantage of novel, close-packed 1000-channel silicon probes originally developed by the Synthetic Neurobiology Group at MIT. These ultra-high-channel-count probes allow for more neurons to be recorded simultaneously, opening up new lines of scientific inquiry, and the dense packing of the electrodes permits spatial oversampling of the neurons, allowing for automated spike sorting techniques with greatly increased capability for tracking individual units. Additionally, LeafLabs' Catkin, a custom 1000 channel neurosensing IC chip (filter, amplification, multiplexing, and analog-digital conversion) has been developed to integrate with these probes, resulting in a probe/headstage combo suitable for use in freely-moving electrophysiology experiments that reduces size, weight, and cost each by a factor of 10 compared to commercially available headstages. Currently, the Catkin probe/headstage combo is tethered to the Willow DAQ system by an ultra thin and lightweight cable (a single shielded 32 AWG twisted pair), resulting in a system ideal for many prolonged freely moving experiments. However, in certain behavioral setups, even a lightweight, minimalist cable is undesirable. For example, because the high data rates are incompatible with approaches used to manage the cable tether (commutators), researchers must occasionally intervene to de-tangle cables; or, the presence of a tether may result in altered behavior from animals. To address these needs, this application proposes the development of a first-of-its-kind fully untethered 1000-channel-simultaneous 30kHz in-vivo electrophysiological recording module, to be made available as a lightweight add-on to the extant Willow system.

项目摘要 记录单个神经元分辨率和毫秒的时间尺度精度的全长活性对 了解单个细胞和复杂的神经回路如何在时间和空间中相互作用。同时 需要记录和刺激大脑中分布的大量神经元种群的记录和刺激 严格评估哺乳动物细胞水平的神经计算理论，并扩展纵向 需要记录以建立神经元电路/动力学的一般原则以及如何复杂 神经元活动与行为有关，既可以进一步促进我们对大脑的基本理解，又要进一步 神经系统和精神病的根本原因，例如阿尔茨海默氏症，帕金森氏症，TBI， 癫痫，抑郁症，并帮助发展新颖和更有效的治疗方法。 这些是大脑倡议的关键目标，也是Leflabs Willow背后的驱动力 电生理记录系统旨在利用新颖的，紧密包装的1000通道硅 麻省理工学院合成神经生物学组最初发现的问题。这些超高通道计数 问题可以简单记录更多的神经元，开辟了新的科学询问线，并 电极的致密堆积允许神经元的空间过采样，从而可以自动尖峰 对跟踪单个单元的能力的分类技术大大提高。 此外，Leflabs的Catkin，一种自定义的1000通道神经传感IC芯片（过滤器，放大，多路复用， 已经开发了与这些问题集成的模拟数字转换），从而导致 探针/媒体组合适用于降低大小的自由移动电生理实验， 与市售的震级相比，重量和成本为10倍。目前，猫 探针/媒体组合由超薄且轻巧的电缆束缚在柳树DAQ系统上（单个） 屏蔽了32个AWG扭曲的一对），从而产生了一个非常适合许多长时间免费移动实验的系统。 但是，在某些行为设置中，即使是轻巧的极简电缆也是不可取的。例如， 因为高数据速率与用于管理电缆系绳的方法不相容 （换向者），研究人员偶尔必须干预DENANGLE电缆；或者，系绳的存在可能 导致动物行为改变。为了满足这些需求，本申请提出了 首先是完全不受束缚的1000通道 - 含量30kHz体内生理生理学 录制模块，可作为轻巧附加组件在willow系统的范围内提供。