Microfluidic Platform for Cancer Cell Culture and Analysis
用于癌细胞培养和分析的微流控平台
基本信息
- 批准号:7434892
- 负责人:
- 金额:$ 31.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-06-01 至 2011-03-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAreaAutomationBiological AssayBioreactorsBreast Cancer CellCancer BiologyCancer cell lineCell physiologyCellsCollaborationsCompatibleComplexConditionConditioned Culture MediaConsumptionCultured CellsData QualityDiagnosisDoseDrug ExposureEngineeringEnvironmentEpithelial CellsExtracellular MatrixFibroblastsGoalsGray unit of radiation doseHumanIn VitroIndividualInstitutesKineticsLaboratoriesLettersLifeLiquid substanceMEKsMalignant NeoplasmsMethodsMicrofabricationMicrofluidic MicrochipsMicrofluidicsOperative Surgical ProceduresOpticsPathway interactionsPatientsPerfusionPharmaceutical PreparationsPharmacologic SubstancePhasePhenotypePliabilityProcessPropertyPumpPurposeRNA InterferenceReagentResearchRoboticsSamplingScheduleScreening for cancerScreening procedureSmall Business Funding MechanismsSmall Business Innovation Research GrantSolutionsStagingStandards of Weights and MeasuresStromal CellsSystemSystems AnalysisTechnologyTherapeuticTimeToxic effectWorkbasecancer cellcommercial applicationcostdesignimprovedin vivoinnovationinstrumentinstrumentationnanolitrenanolitre scalenovelpressureprogramsprototyperesponsescale upshear stresstool
项目摘要
DESCRIPTION (provided by applicant): This Phase II SBIR proposes to develop a microfluidic cell culture and analysis platform for the in vitro screening of cancer cells. The end result of this research will be to deliver a fully functional system (automated instrument and disposable microfluidics) that can be used in both research and pharmaceutical labs. This will be validated and applied to the research underway at our collaborating institute for the purpose of profiling the Raf-MEK-ERK pathway in a panel of ~60 breast cancer cell lines for improved prediction of therapeutic response.
The microfluidic platform will provide key advantages over the current cell based screening technology (96-well plate based), including: 1) improved handling of small cell samples (micoliters per array), 2) the ability to design more relevant microenvironments for phenotype analysis, 3) enabling multiplexed continuous flow experimentation, and 4) 10X-100X reduction of time and cost for cell culture automation. In addition, the platform is designed such that application specific microfluidic arrays can be utilized with a single system, increasing the flexibility and impact of the technology.
The first major aim of this project will be to engineer an automated microfluidic screening platform. The main tasks are to optimize the design of the Phase I prototype, scale-up to a 384 well format, and refine the control system for high throughput operation. Three key innovations developed in our previous work will be further expanded to complete this aim: 1) the design of microfluidic networks and perfusion barriers to better approximate in vivo culture conditions, 2) the use of a pneumatic pressure driven manifold for multiplexed, non-wetted pumping of nanoliter volumes, and 3) the fabrication process that enables formatting the microfluidic arrays to SBS standards, making it compatible with current 96 and 384 well robotic screening instrumentation.
The second major aim will be to apply this system to the cancer cell screening program at our collaborating institute. This will address areas where microfluidic technology can offer enabling benefits not possible with existing tools. Specific applications include: 1) flow based drug exposure, 2) cell invasion assay, 3) 3D ECM culture, 4) medium conditioning by stromal cells, and 5) integration with RNAi methods.
描述(由申请人提供):本II期SBIR计划开发用于体外筛选癌细胞的微流控细胞培养和分析平台。这项研究的最终结果将是提供一个功能齐全的系统(自动化仪器和一次性微流体),可用于研究和制药实验室。这将被验证并应用于我们合作研究所正在进行的研究,目的是在约60个乳腺癌细胞系中分析Raf-MEK-ERK途径,以改进治疗反应的预测。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Philip J Lee其他文献
Philip J Lee的其他文献
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{{ truncateString('Philip J Lee', 18)}}的其他基金
Microfluidic Liver Array for Long Term In Vitro Hepatocyte Culture and Screening
用于长期体外肝细胞培养和筛选的微流控肝脏阵列
- 批准号:
8211767 - 财政年份:2011
- 资助金额:
$ 31.26万 - 项目类别:
Microfluidic Liver Array for Long Term In Vitro Hepatocyte Culture and Screening
用于长期体外肝细胞培养和筛选的微流控肝脏阵列
- 批准号:
8214518 - 财政年份:2011
- 资助金额:
$ 31.26万 - 项目类别:
Microfluidic Liver Array for Long Term In Vitro Hepatocyte Culture and Screening
用于长期体外肝细胞培养和筛选的微流控肝脏阵列
- 批准号:
7908232 - 财政年份:2010
- 资助金额:
$ 31.26万 - 项目类别:
Microfluidic Platform for Cancer Cell Culture and Analysis
用于癌细胞培养和分析的微流控平台
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7622604 - 财政年份:2006
- 资助金额:
$ 31.26万 - 项目类别:
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7085041 - 财政年份:2006
- 资助金额:
$ 31.26万 - 项目类别:
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