PRECISELY CONTROLLED GRADIENTS FOR AXON GUIDANCE
PRECISELY CONTROLLED GRADIENTS FOR AXON GUIDANCE
批准号:
6351894
负责人:
GEOFFREY J GOODHILL
金额:
$11.14万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-02-01 至 2002-01-31
中文摘要
描述(摘自申请人摘要):随着大脑的发育,
英文摘要
DESCRIPTION (Verbatim from the Applicant's Abstract): As the brain develops,
axons often grow over long distances to find appropriate targets. Some of the
most important guidance cues they use to achieve this feat are concentration
gradients of chemotropic molecules. These gradients are detected by the growth
cone, a complex and sensitive structure at the tip of the developing axon.
Understanding how growth cones detect gradients is crucial for understanding
how the brain normally develops, how defects in normal wiring can occur, and
how axons can appropriately regenerate after injury. However, there is
currently very little quantitative information about how growth cones respond
to gradients. This is primarily due to the absence of an experimental technique
for establishing gradients of diffusible axon guidance molecules in vitro, such
that the shape and steepness of the gradient is under the control of the
experimenter. Without such a technique it is impossible to systematically vary
the parameters of the gradient, and thus impossible to determine quantitatively
how each of these parameters regulates axon guidance. The goal of this project
is to develop and implement a novel technique to make these measurements
possible.
We will establish gradients by "printing" chemotropic molecules onto the
surface of a long, thin block of collagen gel in which axons are growing, and
allowing the molecules to diffuse into the collagen. Precisely controlled
volumes of chemotropic factor can be deposited at precisely controlled
locations using a method similar to that employed in inkjet printers. Because
molecular movement by diffusion is fast over short distances but slow over long
distances, initial local unevenness in the concentration smoothes out in a few
hours, leaving behind a gradient that remains smooth and stable for several
weeks. We thus have complete control over the shape of this gradient via
control over the pattern of molecules deposited on the surface. By observing
axon growth and turning behavior in these gradients we will obtain quantitative
data of unprecedented precision on the parameters controlling axon guidance by
diffusible gradients. Our initial model system will be the guidance of rat
dorsal root ganglion axons by gradients of Nerve Growth Factor. However, we
expect this technology to be also widely applicable to other model systems for
axon guidance, and more generally to studies of cell motility and chemotaxis.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Whole-Brain Functional Imaging and Analysis of Zebrafish Sleep
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批准号:10772327
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项目类别:
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资助金额:$16.83万
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财政年份:2022
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负责人:GEOFFREY J GOODHILL
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依托单位:
Whole-Brain Functional Imaging and Analysis of Zebrafish Sleep
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批准号:10430595
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Mechanisms of retinotectal map development
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批准号:6596493
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项目类别:
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资助金额:$11.64万
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财政年份:2003
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负责人:GEOFFREY J GOODHILL
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依托单位:
Mechanisms of retinotectal map development
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批准号:6706971
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项目类别:
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资助金额:$11.64万
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财政年份:2003
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负责人:GEOFFREY J GOODHILL
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依托单位:
CRCNS:Mechanisms of Axonal Gradient Detection
-
批准号:6641501
-
项目类别:
-
资助金额:$23.7万
-
财政年份:2002
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负责人:GEOFFREY J GOODHILL
-
依托单位:
CRCNS:Mechanisms of Axonal Gradient Detection
-
批准号:6796554
-
项目类别:
-
资助金额:$4.15万
-
财政年份:2002
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
CRCNS:Mechanisms of Axonal Gradient Detection
-
批准号:6666807
-
项目类别:
-
资助金额:$23.63万
-
财政年份:2002
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
PRECISELY CONTROLLED GRADIENTS FOR AXON GUIDANCE
-
批准号:6028296
-
项目类别:
-
资助金额:$11.38万
-
财政年份:2000
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
THE DEVELOPMENT & STRUCTURE OF VISUAL CORTICAL MAPS
-
批准号:6524967
-
项目类别:
-
资助金额:$13.55万
-
财政年份:1999
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
THE DEVELOPMENT & STRUCTURE OF VISUAL CORTICAL MAPS
-
批准号:6384808
-
项目类别:
-
资助金额:$13.16万
-
财政年份:1999
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
THE DEVELOPMENT AND STRUCTURE OF VISUAL CORTICAL MAPS
-
批准号:2843600
-
项目类别:
-
资助金额:$13.43万
-
财政年份:1999
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
THE DEVELOPMENT & STRUCTURE OF VISUAL CORTICAL MAPS
-
批准号:6179897
-
项目类别:
-
资助金额:$12.77万
-
财政年份:1999
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
PRECISELY CONTROLLED GRADIENTS FOR AXON GUIDANCE
-
批准号:2875516
-
项目类别:
-
资助金额:$10.0万
-
财政年份:1998
-
负责人:GEOFFREY J GOODHILL
-
依托单位:
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