ELECTRON MICROSCOPY CORE

电子显微镜（EM）核心是NIDA赞助的成瘾中心的关键组成部分， 提供有助于发射机的蜂窝和亚蜂窝定位的资源和服务， 受体和转运蛋白正在研究中。这些对所有的基础科学研究都很重要， 项目1-3，对于项目3中的定量电子显微镜免疫标记至关重要。核心 人员包括两名神经病学和神经科学系的教员，威尔医疗 康奈尔大学学院。Pickel博士开创并建立了预嵌入的许多方面 EM免疫标记中枢神经系统的多种抗原，并且还具有 在脑解剖学和LM检测mRNA和蛋白质方面的大量专业知识正在研究中， 项目1和2。Glass博士在使用定量分析方法方面做出了重大贡献。 成瘾物质影响下受体运输的形态计量学分析。的资源 可在核心包括CM-10和Techni电子显微镜以及所有较小的设备 为LM和EM结构分析植入种子。核心设施将提供与下列方面有关的服务和设施： 抗血清的表征和用于LM和EM免疫标记的组织的处理， （前）或（后）包埋在树脂中以进行显微镜分析，（B）在细胞表面上的mRNA的原位杂交， 冷冻载玻片脑切片，（c）免疫标记的图像采集和定量。核心 教师和资源位于街对面的康奈尔大学威尔医学院 来自洛克菲勒大学，占据了基普湾大楼的一层。进入该设施很容易 洛克菲勒大学的同事通过出示适当的身份证明获得了这一权利。 EM资源的目标是： 1.提供特定脑区组织的最先进超微结构检查，采用标准 和新形式的电子显微镜和其他标准成像技术，为中心，包括 项目3，可能包括项目1和项目2，以及任何需要这种技术的试点项目。 2.根据需要开发超微结构成像的新技术。 3.对科学家和技术支持人员进行电子显微镜和相关技术方面的培训 成像技术。

The Electron Microscopy (EM) Core is a critical component of the NIDA sponsored addiction center, which provides resources and services contributing to the cellular and subcellular localization of transmitters, receptors and transporters under investigation. These are important for all the basic science research in Projects 1-3, and crucial for the quantitative electron microscopic immunolabeling in Project 3. The core personnel include two faculty members of the Department of Neurology and Neuroscience, Weill Medical College of Cornell University. The Director, Dr. Pickel, has pioneered and established many facets of preembedding EM immunolabeling of a variety of antigens in the central nervous system, and also has substantial expertise in brain anatomy and LM detection of mRNA and proteins under investigation in Projects 1 and 2. The co-director, Dr. Glass has made significant contributions to the use of quantitative morphometric analysis of receptor trafficking under the influence of addictive substances. The resources available in the core include a CM-10 and Techni electron microscopes as well as all smaller equipment seeded for LM and EM structural analysis. The core will provide services and facilities related to (a) characterization of antisera and processing of the tissue for both LM and EM immunolabeling either before (pre-) or after (post-) embedding in resin to for microscopic analysis, (b) in situ hybridization of mRNA on frozen slide-mounted brain sections, (c) image acquisition and quantification of immunolabeling. The Core faculty and resources are located at Weill Medical College of Cornell University, which is across the street from Rockefeller University, and occupies one floor of the Kips Bay building. Access to this facility is easily gained by Rockefeller University associates with the presentation of proper identification. The Goals of the EM Resourse are: 1. To offer state of the art ultrastructural examination of tissues from specific brain region, utilizing standard and novel forms of electron microscopy and other standard imaging techniques, for the Center, including Project 3, possibly Projects 1 and 2, and any pilot projects which require this technology. 2. To develop new technologies for ultrastructural imaging as needed. 3. To train scientists and technical support staff in the various techniques of electron microscopy and related imaging techniques.