ISLET PROCUREMENT AND ANALYSIS CORE

胰岛获取和分析核心是范德比尔特DRTC的新核心。胰岛生物学，发育， 和功能是DRTC的主要研究领域，研究人员研究了各种胰岛相关过程 从细胞内信号传导、1型糖尿病的免疫学、胰岛富集转录因子， 胰岛移植这些DRTC附属研究人员的许多实验范式的核心是 接触啮齿动物、猪或人胰岛。这个核心的目标是促进研究 通过提供高质量、特征良好的胰岛， 使用这些胰岛进行研究的研究人员。范德比尔特的一个小鼠胰岛分离中心已经投入使用 在范德比尔特小鼠代谢表型中心（MMPC）的赞助下， 由于DRTC的数量和活性， 范德比尔特内部的调查人员不断增加。此外，多名范德比尔特研究人员正在研究 参与小鼠胰岛发育和功能的机制，但对人类胰岛的限制减缓了 将他们的观察转化为人类胰岛竞技场。因此，在本申请中，我们提出创建 范德比尔特岛采购和分析核心，这将得到NIDDK资助的 MMPC和DRTC，并将大大增加DRTC附属研究者的访问， 胰岛该核心提供的服务是：1）啮齿动物胰岛的分离; 2）纯化 和人胰岛的处理（从NIH/JDRF人胰岛分布计划接收）;和3） 通过评估细胞灌流中的胰岛素分泌来表征来自所有物种的胰岛制备物 系统该核心将扩大DRTC附属研究人员进行糖尿病相关研究的能力 并将与其他DRTC支持的内核进行交互。例如，分离的胰岛可以在细胞中进行研究。 成像共享资源，分泌的胰岛激素将在激素测定核心中测定，或 评估体内胰岛素分泌的平行研究可在代谢生理学共享 来源和相关的体外研究在孤立的胰岛。该核心将提供基本服务， 在下一个资助周期支持DRTC附属研究人员的研究。

The Islet Procurement and Analysis Core is a new core of the Vanderbilt DRTC. Islet biology, development, and function are major DRTC research areas with investigators studying a variety of islet-related processes ranging from intracellular signaling, the immunology of type 1 diabetes, islet-enriched transcription factors, to islet transplantation. Central to many experimental paradigms of these DRTC-affiliated investigators is access to rodent, porcine, or human pancreatic islets. The objective of this core is to facilitate the research of DRTC-affiliated investigators by providing high quality, well-characterized pancreatic islets and to assist investigators in studies that use these islets. A mouse islet isolation core at Vanderbilt has been operational under the auspices of the Vanderbilt Mouse Metabolic Phenotyping Center (MMPC), but its design is such that it is not well-suited for handling the volume of islets needed as the number and activity of DRTC investigators within Vanderbilt continue to grow. In addition, a number of Vanderbilt researchers are studying the mechanisms involved in mouse islet development and function, but limited access to human islets slows translation of their observations into the human islet arena. Thus, in this application, we propose to create the Vanderbilt Islet Procurement and Analysis Core, which will be jointly supported by the NIDDK-funded MMPC and the DRTC and will substantially increase the access of DRTC-affiliated investigators to pancreatic islets. The services offered by this core are: 1) isolation of rodent pancreatic islets; 2) purification and handling of human islets (received from NIH/JDRF human islet distribution program); and 3) characterization of islet preparations from all species by assessment of insulin secretion in a cell perifusion system. This core will expand the ability of DRTC-affiliated investigators to perform diabetes-related research and will interact with other DRTC-supported cores. For example, isolated islets may be studied in the Cell Imaging Shared Resource, secreted islet hormones would be assayed in the Hormone Assay Core, or parallel studies assessing in vivo insulin secretion can be performed in the Metabolic Physiology Shared Resource and correlated with in vitro studies in isolated islets. This core will provide essential services that support the research of DRTC-affiliated investigators in the next funding cycle.